Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS17955 and RPSI07_RS17960 are separated by 166 nucleotides RPSI07_RS17960 and RPSI07_RS17965 are separated by 30 nucleotides
RPSI07_RS17955: RPSI07_RS17955 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 2,233,520 to 2,234,809
_RS17955
RPSI07_RS17960: RPSI07_RS17960 - MurR/RpiR family transcriptional regulator, at 2,234,976 to 2,235,893
_RS17960
RPSI07_RS17965: RPSI07_RS17965 - oxidoreductase, at 2,235,924 to 2,236,997
_RS17965
Position (kb)
2234
2235
2236 Strain fitness (log2 ratio)
-2
-1
0
1 at 2234.357 kb on + strand at 2234.358 kb on - strand, within RPSI07_RS17955 at 2234.358 kb on - strand, within RPSI07_RS17955 at 2234.496 kb on + strand, within RPSI07_RS17955 at 2234.573 kb on + strand, within RPSI07_RS17955 at 2234.573 kb on + strand, within RPSI07_RS17955 at 2234.573 kb on + strand, within RPSI07_RS17955 at 2234.574 kb on - strand, within RPSI07_RS17955 at 2234.574 kb on - strand, within RPSI07_RS17955 at 2234.574 kb on - strand, within RPSI07_RS17955 at 2234.574 kb on - strand, within RPSI07_RS17955 at 2234.595 kb on + strand, within RPSI07_RS17955 at 2234.595 kb on + strand, within RPSI07_RS17955 at 2234.595 kb on + strand, within RPSI07_RS17955 at 2234.596 kb on - strand, within RPSI07_RS17955 at 2234.596 kb on - strand, within RPSI07_RS17955 at 2234.596 kb on - strand, within RPSI07_RS17955 at 2234.676 kb on + strand, within RPSI07_RS17955 at 2234.893 kb on - strand at 2234.972 kb on + strand at 2234.972 kb on + strand at 2234.973 kb on - strand at 2234.973 kb on - strand at 2234.973 kb on - strand at 2234.973 kb on - strand at 2234.973 kb on - strand at 2234.973 kb on - strand at 2235.129 kb on + strand, within RPSI07_RS17960 at 2235.130 kb on - strand, within RPSI07_RS17960 at 2235.130 kb on - strand, within RPSI07_RS17960 at 2235.130 kb on - strand, within RPSI07_RS17960 at 2235.310 kb on - strand, within RPSI07_RS17960 at 2235.310 kb on - strand, within RPSI07_RS17960 at 2235.310 kb on - strand, within RPSI07_RS17960 at 2235.652 kb on - strand, within RPSI07_RS17960 at 2235.868 kb on - strand at 2235.891 kb on + strand at 2236.099 kb on - strand, within RPSI07_RS17965 at 2236.099 kb on - strand, within RPSI07_RS17965 at 2236.099 kb on - strand, within RPSI07_RS17965 at 2236.099 kb on - strand, within RPSI07_RS17965 at 2236.099 kb on - strand, within RPSI07_RS17965 at 2236.108 kb on - strand, within RPSI07_RS17965 at 2236.220 kb on - strand, within RPSI07_RS17965 at 2236.333 kb on - strand, within RPSI07_RS17965 at 2236.638 kb on + strand, within RPSI07_RS17965 at 2236.638 kb on + strand, within RPSI07_RS17965 at 2236.638 kb on + strand, within RPSI07_RS17965 at 2236.638 kb on + strand, within RPSI07_RS17965 at 2236.639 kb on - strand, within RPSI07_RS17965
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 2,234,357 + +0.1 2,234,358 - RPSI07_RS17955 0.65 -0.7 2,234,358 - RPSI07_RS17955 0.65 +0.1 2,234,496 + RPSI07_RS17955 0.76 -0.1 2,234,573 + RPSI07_RS17955 0.82 -1.7 2,234,573 + RPSI07_RS17955 0.82 -0.5 2,234,573 + RPSI07_RS17955 0.82 +0.8 2,234,574 - RPSI07_RS17955 0.82 -1.5 2,234,574 - RPSI07_RS17955 0.82 -0.9 2,234,574 - RPSI07_RS17955 0.82 -0.7 2,234,574 - RPSI07_RS17955 0.82 -1.6 2,234,595 + RPSI07_RS17955 0.83 +1.0 2,234,595 + RPSI07_RS17955 0.83 +1.0 2,234,595 + RPSI07_RS17955 0.83 +1.1 2,234,596 - RPSI07_RS17955 0.83 -0.2 2,234,596 - RPSI07_RS17955 0.83 -0.2 2,234,596 - RPSI07_RS17955 0.83 +0.1 2,234,676 + RPSI07_RS17955 0.90 +1.1 2,234,893 - -0.6 2,234,972 + -1.8 2,234,972 + +0.2 2,234,973 - -0.2 2,234,973 - -2.2 2,234,973 - -0.4 2,234,973 - +0.7 2,234,973 - +0.1 2,234,973 - -2.2 2,235,129 + RPSI07_RS17960 0.17 -0.1 2,235,130 - RPSI07_RS17960 0.17 +0.6 2,235,130 - RPSI07_RS17960 0.17 +0.2 2,235,130 - RPSI07_RS17960 0.17 -0.6 2,235,310 - RPSI07_RS17960 0.36 -1.4 2,235,310 - RPSI07_RS17960 0.36 -0.6 2,235,310 - RPSI07_RS17960 0.36 -0.3 2,235,652 - RPSI07_RS17960 0.74 -0.2 2,235,868 - +1.6 2,235,891 + +0.2 2,236,099 - RPSI07_RS17965 0.16 -0.7 2,236,099 - RPSI07_RS17965 0.16 +1.7 2,236,099 - RPSI07_RS17965 0.16 +0.1 2,236,099 - RPSI07_RS17965 0.16 -1.6 2,236,099 - RPSI07_RS17965 0.16 -1.6 2,236,108 - RPSI07_RS17965 0.17 -0.6 2,236,220 - RPSI07_RS17965 0.28 -0.4 2,236,333 - RPSI07_RS17965 0.38 -0.8 2,236,638 + RPSI07_RS17965 0.66 +0.4 2,236,638 + RPSI07_RS17965 0.66 -0.8 2,236,638 + RPSI07_RS17965 0.66 -0.9 2,236,638 + RPSI07_RS17965 0.66 +0.6 2,236,639 - RPSI07_RS17965 0.67 -1.3
Or see this region's nucleotide sequence