Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS14865 and RPSI07_RS14870 are separated by 177 nucleotides RPSI07_RS14870 and RPSI07_RS14875 are separated by 83 nucleotides
RPSI07_RS14865: RPSI07_RS14865 - fumarate hydratase, at 1,504,447 to 1,505,970
_RS14865
RPSI07_RS14870: RPSI07_RS14870 - membrane protein, at 1,506,148 to 1,506,687
_RS14870
RPSI07_RS14875: RPSI07_RS14875 - EamA/RhaT family transporter, at 1,506,771 to 1,507,781
_RS14875
Position (kb)
1506
1507 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 1505.268 kb on - strand, within RPSI07_RS14865 at 1505.864 kb on + strand at 1505.865 kb on - strand at 1505.865 kb on - strand at 1505.888 kb on + strand at 1505.888 kb on + strand at 1505.889 kb on - strand at 1505.889 kb on - strand at 1505.903 kb on + strand at 1505.906 kb on + strand at 1505.906 kb on + strand at 1505.906 kb on + strand at 1505.906 kb on + strand at 1505.906 kb on + strand at 1505.906 kb on + strand at 1505.907 kb on - strand at 1505.907 kb on - strand at 1505.915 kb on + strand at 1505.916 kb on - strand at 1505.916 kb on - strand at 1505.916 kb on - strand at 1505.988 kb on + strand at 1506.438 kb on - strand, within RPSI07_RS14870 at 1506.438 kb on - strand, within RPSI07_RS14870 at 1506.575 kb on + strand, within RPSI07_RS14870 at 1506.575 kb on + strand, within RPSI07_RS14870 at 1506.576 kb on - strand, within RPSI07_RS14870 at 1506.576 kb on - strand, within RPSI07_RS14870 at 1506.581 kb on + strand, within RPSI07_RS14870 at 1506.581 kb on + strand, within RPSI07_RS14870 at 1506.581 kb on + strand, within RPSI07_RS14870 at 1506.581 kb on + strand, within RPSI07_RS14870 at 1506.581 kb on + strand, within RPSI07_RS14870 at 1506.581 kb on + strand, within RPSI07_RS14870 at 1506.581 kb on + strand, within RPSI07_RS14870 at 1506.581 kb on + strand, within RPSI07_RS14870 at 1506.581 kb on + strand, within RPSI07_RS14870 at 1506.581 kb on + strand, within RPSI07_RS14870 at 1506.582 kb on - strand, within RPSI07_RS14870 at 1506.582 kb on - strand, within RPSI07_RS14870 at 1506.611 kb on + strand, within RPSI07_RS14870 at 1506.612 kb on - strand, within RPSI07_RS14870 at 1506.612 kb on - strand, within RPSI07_RS14870 at 1506.612 kb on - strand, within RPSI07_RS14870 at 1506.619 kb on + strand, within RPSI07_RS14870 at 1506.619 kb on + strand, within RPSI07_RS14870 at 1506.620 kb on - strand, within RPSI07_RS14870 at 1506.699 kb on + strand at 1506.699 kb on + strand at 1506.699 kb on + strand at 1506.700 kb on - strand at 1506.700 kb on - strand at 1506.723 kb on + strand at 1506.723 kb on + strand at 1506.724 kb on - strand at 1506.944 kb on - strand, within RPSI07_RS14875 at 1506.944 kb on - strand, within RPSI07_RS14875 at 1507.057 kb on + strand, within RPSI07_RS14875 at 1507.057 kb on + strand, within RPSI07_RS14875 at 1507.058 kb on - strand, within RPSI07_RS14875 at 1507.165 kb on + strand, within RPSI07_RS14875 at 1507.166 kb on - strand, within RPSI07_RS14875 at 1507.166 kb on - strand, within RPSI07_RS14875 at 1507.222 kb on + strand, within RPSI07_RS14875 at 1507.223 kb on - strand, within RPSI07_RS14875 at 1507.223 kb on - strand, within RPSI07_RS14875 at 1507.322 kb on - strand, within RPSI07_RS14875
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,505,268 - RPSI07_RS14865 0.54 -0.5 1,505,864 + -1.3 1,505,865 - +0.1 1,505,865 - +0.1 1,505,888 + -1.3 1,505,888 + +6.7 1,505,889 - +0.5 1,505,889 - +0.4 1,505,903 + +0.1 1,505,906 + -0.6 1,505,906 + +0.7 1,505,906 + +1.6 1,505,906 + +1.1 1,505,906 + +1.1 1,505,906 + +0.1 1,505,907 - +1.3 1,505,907 - -1.7 1,505,915 + +2.7 1,505,916 - -0.6 1,505,916 - +0.1 1,505,916 - -0.5 1,505,988 + -0.3 1,506,438 - RPSI07_RS14870 0.54 +2.7 1,506,438 - RPSI07_RS14870 0.54 -0.7 1,506,575 + RPSI07_RS14870 0.79 -1.7 1,506,575 + RPSI07_RS14870 0.79 -1.1 1,506,576 - RPSI07_RS14870 0.79 +0.1 1,506,576 - RPSI07_RS14870 0.79 +2.7 1,506,581 + RPSI07_RS14870 0.80 -0.9 1,506,581 + RPSI07_RS14870 0.80 -1.7 1,506,581 + RPSI07_RS14870 0.80 +3.4 1,506,581 + RPSI07_RS14870 0.80 +0.6 1,506,581 + RPSI07_RS14870 0.80 +0.4 1,506,581 + RPSI07_RS14870 0.80 -1.9 1,506,581 + RPSI07_RS14870 0.80 +2.0 1,506,581 + RPSI07_RS14870 0.80 -0.7 1,506,581 + RPSI07_RS14870 0.80 -0.1 1,506,581 + RPSI07_RS14870 0.80 +0.8 1,506,582 - RPSI07_RS14870 0.80 -0.3 1,506,582 - RPSI07_RS14870 0.80 -0.5 1,506,611 + RPSI07_RS14870 0.86 -1.0 1,506,612 - RPSI07_RS14870 0.86 -0.6 1,506,612 - RPSI07_RS14870 0.86 -0.5 1,506,612 - RPSI07_RS14870 0.86 -0.1 1,506,619 + RPSI07_RS14870 0.87 +0.8 1,506,619 + RPSI07_RS14870 0.87 -0.5 1,506,620 - RPSI07_RS14870 0.87 +0.2 1,506,699 + +0.1 1,506,699 + -0.8 1,506,699 + -0.7 1,506,700 - -1.4 1,506,700 - -1.5 1,506,723 + +0.7 1,506,723 + -0.5 1,506,724 - -0.5 1,506,944 - RPSI07_RS14875 0.17 +0.9 1,506,944 - RPSI07_RS14875 0.17 +1.1 1,507,057 + RPSI07_RS14875 0.28 -1.1 1,507,057 + RPSI07_RS14875 0.28 -0.6 1,507,058 - RPSI07_RS14875 0.28 -1.1 1,507,165 + RPSI07_RS14875 0.39 -1.8 1,507,166 - RPSI07_RS14875 0.39 +0.4 1,507,166 - RPSI07_RS14875 0.39 -1.2 1,507,222 + RPSI07_RS14875 0.45 +0.1 1,507,223 - RPSI07_RS14875 0.45 -0.5 1,507,223 - RPSI07_RS14875 0.45 +0.4 1,507,322 - RPSI07_RS14875 0.55 -0.7
Or see this region's nucleotide sequence