Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS13910

Experiment: SMSA with Ralstonia_antibiotic_mix

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS13900 and RPSI07_RS13905 are separated by 289 nucleotidesRPSI07_RS13905 and RPSI07_RS13910 are separated by 207 nucleotidesRPSI07_RS13910 and RPSI07_RS13915 overlap by 11 nucleotidesRPSI07_RS13915 and RPSI07_RS13920 overlap by 8 nucleotides RPSI07_RS13900: RPSI07_RS13900 - esterase, at 1,300,717 to 1,301,748 _RS13900 RPSI07_RS13905: RPSI07_RS13905 - peptidoglycan-binding protein LysM, at 1,302,038 to 1,302,520 _RS13905 RPSI07_RS13910: RPSI07_RS13910 - hypothetical protein, at 1,302,728 to 1,303,222 _RS13910 RPSI07_RS13915: RPSI07_RS13915 - hypothetical protein, at 1,303,212 to 1,303,928 _RS13915 RPSI07_RS13920: RPSI07_RS13920 - type II/IV secretion system protein, at 1,303,921 to 1,305,546 _RS13920 Position (kb) 1302 1303 1304Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1301.754 kb on + strandat 1301.755 kb on - strandat 1301.755 kb on - strandat 1301.801 kb on + strandat 1301.801 kb on + strandat 1301.801 kb on + strandat 1301.802 kb on - strandat 1301.966 kb on - strandat 1302.173 kb on + strand, within RPSI07_RS13905at 1302.173 kb on + strand, within RPSI07_RS13905at 1302.173 kb on + strand, within RPSI07_RS13905at 1302.174 kb on - strand, within RPSI07_RS13905at 1302.174 kb on - strand, within RPSI07_RS13905at 1302.174 kb on - strand, within RPSI07_RS13905at 1302.368 kb on - strand, within RPSI07_RS13905at 1302.414 kb on - strand, within RPSI07_RS13905at 1302.414 kb on - strand, within RPSI07_RS13905at 1302.485 kb on + strandat 1302.486 kb on - strandat 1302.518 kb on + strandat 1302.518 kb on + strandat 1302.518 kb on + strandat 1302.518 kb on + strandat 1302.518 kb on + strandat 1302.518 kb on + strandat 1302.518 kb on + strandat 1302.519 kb on - strandat 1302.519 kb on - strandat 1302.519 kb on - strandat 1302.519 kb on - strandat 1302.519 kb on - strandat 1302.649 kb on - strandat 1302.760 kb on - strandat 1302.760 kb on - strandat 1302.760 kb on - strandat 1302.774 kb on + strandat 1303.033 kb on + strand, within RPSI07_RS13910at 1303.459 kb on + strand, within RPSI07_RS13915at 1303.460 kb on - strand, within RPSI07_RS13915at 1303.544 kb on - strand, within RPSI07_RS13915at 1303.544 kb on - strand, within RPSI07_RS13915at 1303.741 kb on + strand, within RPSI07_RS13915at 1303.742 kb on - strand, within RPSI07_RS13915at 1303.918 kb on + strandat 1303.919 kb on - strandat 1303.919 kb on - strandat 1303.919 kb on - strandat 1304.115 kb on + strand, within RPSI07_RS13920at 1304.136 kb on - strand, within RPSI07_RS13920

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix
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1,301,754 + -1.6
1,301,755 - -0.3
1,301,755 - -2.7
1,301,801 + +1.7
1,301,801 + +0.1
1,301,801 + +0.4
1,301,802 - +1.0
1,301,966 - -0.9
1,302,173 + RPSI07_RS13905 0.28 -0.2
1,302,173 + RPSI07_RS13905 0.28 -0.1
1,302,173 + RPSI07_RS13905 0.28 -0.8
1,302,174 - RPSI07_RS13905 0.28 -2.6
1,302,174 - RPSI07_RS13905 0.28 -1.2
1,302,174 - RPSI07_RS13905 0.28 -0.7
1,302,368 - RPSI07_RS13905 0.68 -1.4
1,302,414 - RPSI07_RS13905 0.78 +0.4
1,302,414 - RPSI07_RS13905 0.78 -1.3
1,302,485 + -1.4
1,302,486 - -0.2
1,302,518 + +0.3
1,302,518 + +0.4
1,302,518 + -0.9
1,302,518 + -0.2
1,302,518 + -0.9
1,302,518 + -1.1
1,302,518 + -0.2
1,302,519 - +0.1
1,302,519 - +1.1
1,302,519 - +0.8
1,302,519 - -0.3
1,302,519 - -1.0
1,302,649 - +2.0
1,302,760 - -0.6
1,302,760 - +2.7
1,302,760 - +0.3
1,302,774 + -0.1
1,303,033 + RPSI07_RS13910 0.62 -0.9
1,303,459 + RPSI07_RS13915 0.34 +0.1
1,303,460 - RPSI07_RS13915 0.35 -0.7
1,303,544 - RPSI07_RS13915 0.46 -0.3
1,303,544 - RPSI07_RS13915 0.46 -0.9
1,303,741 + RPSI07_RS13915 0.74 +2.5
1,303,742 - RPSI07_RS13915 0.74 -0.1
1,303,918 + +1.8
1,303,919 - +1.1
1,303,919 - -0.4
1,303,919 - +0.9
1,304,115 + RPSI07_RS13920 0.12 +3.8
1,304,136 - RPSI07_RS13920 0.13 +0.3

Or see this region's nucleotide sequence