Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS13900 and RPSI07_RS13905 are separated by 289 nucleotides RPSI07_RS13905 and RPSI07_RS13910 are separated by 207 nucleotides RPSI07_RS13910 and RPSI07_RS13915 overlap by 11 nucleotides
RPSI07_RS13900: RPSI07_RS13900 - esterase, at 1,300,717 to 1,301,748
_RS13900
RPSI07_RS13905: RPSI07_RS13905 - peptidoglycan-binding protein LysM, at 1,302,038 to 1,302,520
_RS13905
RPSI07_RS13910: RPSI07_RS13910 - hypothetical protein, at 1,302,728 to 1,303,222
_RS13910
RPSI07_RS13915: RPSI07_RS13915 - hypothetical protein, at 1,303,212 to 1,303,928
_RS13915
Position (kb)
1302
1303 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1301.348 kb on + strand, within RPSI07_RS13900 at 1301.348 kb on + strand, within RPSI07_RS13900 at 1301.348 kb on + strand, within RPSI07_RS13900 at 1301.348 kb on + strand, within RPSI07_RS13900 at 1301.349 kb on - strand, within RPSI07_RS13900 at 1301.349 kb on - strand, within RPSI07_RS13900 at 1301.349 kb on - strand, within RPSI07_RS13900 at 1301.349 kb on - strand, within RPSI07_RS13900 at 1301.349 kb on - strand, within RPSI07_RS13900 at 1301.443 kb on - strand, within RPSI07_RS13900 at 1301.443 kb on - strand, within RPSI07_RS13900 at 1301.443 kb on - strand, within RPSI07_RS13900 at 1301.531 kb on + strand, within RPSI07_RS13900 at 1301.531 kb on + strand, within RPSI07_RS13900 at 1301.531 kb on + strand, within RPSI07_RS13900 at 1301.532 kb on - strand, within RPSI07_RS13900 at 1301.532 kb on - strand, within RPSI07_RS13900 at 1301.541 kb on - strand, within RPSI07_RS13900 at 1301.582 kb on + strand, within RPSI07_RS13900 at 1301.583 kb on - strand, within RPSI07_RS13900 at 1301.583 kb on - strand, within RPSI07_RS13900 at 1301.754 kb on + strand at 1301.755 kb on - strand at 1301.755 kb on - strand at 1301.801 kb on + strand at 1301.801 kb on + strand at 1301.801 kb on + strand at 1301.802 kb on - strand at 1301.966 kb on - strand at 1302.173 kb on + strand, within RPSI07_RS13905 at 1302.173 kb on + strand, within RPSI07_RS13905 at 1302.173 kb on + strand, within RPSI07_RS13905 at 1302.174 kb on - strand, within RPSI07_RS13905 at 1302.174 kb on - strand, within RPSI07_RS13905 at 1302.174 kb on - strand, within RPSI07_RS13905 at 1302.368 kb on - strand, within RPSI07_RS13905 at 1302.414 kb on - strand, within RPSI07_RS13905 at 1302.414 kb on - strand, within RPSI07_RS13905 at 1302.485 kb on + strand at 1302.486 kb on - strand at 1302.518 kb on + strand at 1302.518 kb on + strand at 1302.518 kb on + strand at 1302.518 kb on + strand at 1302.518 kb on + strand at 1302.518 kb on + strand at 1302.518 kb on + strand at 1302.519 kb on - strand at 1302.519 kb on - strand at 1302.519 kb on - strand at 1302.519 kb on - strand at 1302.519 kb on - strand at 1302.649 kb on - strand at 1302.760 kb on - strand at 1302.760 kb on - strand at 1302.760 kb on - strand at 1302.774 kb on + strand at 1303.033 kb on + strand, within RPSI07_RS13910 at 1303.459 kb on + strand, within RPSI07_RS13915 at 1303.460 kb on - strand, within RPSI07_RS13915
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,301,348 + RPSI07_RS13900 0.61 -1.1 1,301,348 + RPSI07_RS13900 0.61 -0.3 1,301,348 + RPSI07_RS13900 0.61 +0.0 1,301,348 + RPSI07_RS13900 0.61 -0.1 1,301,349 - RPSI07_RS13900 0.61 -0.4 1,301,349 - RPSI07_RS13900 0.61 -0.4 1,301,349 - RPSI07_RS13900 0.61 -2.4 1,301,349 - RPSI07_RS13900 0.61 -1.7 1,301,349 - RPSI07_RS13900 0.61 -0.6 1,301,443 - RPSI07_RS13900 0.70 -0.7 1,301,443 - RPSI07_RS13900 0.70 +2.5 1,301,443 - RPSI07_RS13900 0.70 -1.9 1,301,531 + RPSI07_RS13900 0.79 -0.2 1,301,531 + RPSI07_RS13900 0.79 +0.1 1,301,531 + RPSI07_RS13900 0.79 +0.5 1,301,532 - RPSI07_RS13900 0.79 -0.4 1,301,532 - RPSI07_RS13900 0.79 -2.0 1,301,541 - RPSI07_RS13900 0.80 +0.4 1,301,582 + RPSI07_RS13900 0.84 -0.1 1,301,583 - RPSI07_RS13900 0.84 +0.5 1,301,583 - RPSI07_RS13900 0.84 +3.1 1,301,754 + -1.6 1,301,755 - -0.3 1,301,755 - -2.7 1,301,801 + +1.7 1,301,801 + +0.1 1,301,801 + +0.4 1,301,802 - +1.0 1,301,966 - -0.9 1,302,173 + RPSI07_RS13905 0.28 -0.2 1,302,173 + RPSI07_RS13905 0.28 -0.1 1,302,173 + RPSI07_RS13905 0.28 -0.8 1,302,174 - RPSI07_RS13905 0.28 -2.6 1,302,174 - RPSI07_RS13905 0.28 -1.2 1,302,174 - RPSI07_RS13905 0.28 -0.7 1,302,368 - RPSI07_RS13905 0.68 -1.4 1,302,414 - RPSI07_RS13905 0.78 +0.4 1,302,414 - RPSI07_RS13905 0.78 -1.3 1,302,485 + -1.4 1,302,486 - -0.2 1,302,518 + +0.3 1,302,518 + +0.4 1,302,518 + -0.9 1,302,518 + -0.2 1,302,518 + -0.9 1,302,518 + -1.1 1,302,518 + -0.2 1,302,519 - +0.1 1,302,519 - +1.1 1,302,519 - +0.8 1,302,519 - -0.3 1,302,519 - -1.0 1,302,649 - +2.0 1,302,760 - -0.6 1,302,760 - +2.7 1,302,760 - +0.3 1,302,774 + -0.1 1,303,033 + RPSI07_RS13910 0.62 -0.9 1,303,459 + RPSI07_RS13915 0.34 +0.1 1,303,460 - RPSI07_RS13915 0.35 -0.7
Or see this region's nucleotide sequence