Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS13790 and RPSI07_RS13795 are separated by 61 nucleotides RPSI07_RS13795 and RPSI07_RS13800 are separated by 5 nucleotides RPSI07_RS13800 and RPSI07_RS13805 are separated by 102 nucleotides
RPSI07_RS13790: RPSI07_RS13790 - LamB/YcsF family protein, at 1,281,983 to 1,282,726
_RS13790
RPSI07_RS13795: RPSI07_RS13795 - DUF969 domain-containing protein, at 1,282,788 to 1,283,510
_RS13795
RPSI07_RS13800: RPSI07_RS13800 - DUF979 domain-containing protein, at 1,283,516 to 1,284,472
_RS13800
RPSI07_RS13805: RPSI07_RS13805 - C4-dicarboxylate ABC transporter, at 1,284,575 to 1,285,594
_RS13805
Position (kb)
1283
1284
1285 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1282.770 kb on + strand at 1282.770 kb on + strand at 1282.771 kb on - strand at 1282.771 kb on - strand at 1282.793 kb on + strand at 1282.794 kb on - strand at 1282.794 kb on - strand at 1282.794 kb on - strand at 1282.807 kb on + strand at 1282.808 kb on - strand at 1283.097 kb on + strand, within RPSI07_RS13795 at 1283.534 kb on + strand at 1283.535 kb on - strand at 1283.535 kb on - strand at 1283.535 kb on - strand at 1283.535 kb on - strand at 1283.541 kb on - strand at 1284.119 kb on + strand, within RPSI07_RS13800 at 1284.119 kb on + strand, within RPSI07_RS13800 at 1284.120 kb on - strand, within RPSI07_RS13800 at 1284.120 kb on - strand, within RPSI07_RS13800 at 1284.120 kb on - strand, within RPSI07_RS13800 at 1284.158 kb on + strand, within RPSI07_RS13800 at 1284.159 kb on - strand, within RPSI07_RS13800 at 1284.260 kb on + strand, within RPSI07_RS13800 at 1284.260 kb on + strand, within RPSI07_RS13800 at 1284.261 kb on - strand, within RPSI07_RS13800 at 1284.440 kb on - strand at 1284.452 kb on + strand at 1284.453 kb on - strand at 1284.453 kb on - strand at 1284.536 kb on + strand at 1284.536 kb on + strand at 1284.537 kb on - strand at 1284.574 kb on + strand at 1284.575 kb on - strand at 1284.702 kb on - strand, within RPSI07_RS13805 at 1284.938 kb on + strand, within RPSI07_RS13805 at 1284.939 kb on - strand, within RPSI07_RS13805 at 1285.020 kb on - strand, within RPSI07_RS13805 at 1285.020 kb on - strand, within RPSI07_RS13805 at 1285.106 kb on + strand, within RPSI07_RS13805 at 1285.106 kb on + strand, within RPSI07_RS13805 at 1285.107 kb on - strand, within RPSI07_RS13805 at 1285.107 kb on - strand, within RPSI07_RS13805 at 1285.107 kb on - strand, within RPSI07_RS13805 at 1285.108 kb on - strand, within RPSI07_RS13805 at 1285.265 kb on + strand, within RPSI07_RS13805 at 1285.269 kb on - strand, within RPSI07_RS13805 at 1285.271 kb on + strand, within RPSI07_RS13805 at 1285.271 kb on + strand, within RPSI07_RS13805 at 1285.271 kb on + strand, within RPSI07_RS13805 at 1285.271 kb on + strand, within RPSI07_RS13805 at 1285.272 kb on - strand, within RPSI07_RS13805 at 1285.272 kb on - strand, within RPSI07_RS13805 at 1285.272 kb on - strand, within RPSI07_RS13805 at 1285.272 kb on - strand, within RPSI07_RS13805 at 1285.415 kb on + strand, within RPSI07_RS13805 at 1285.416 kb on - strand, within RPSI07_RS13805 at 1285.416 kb on - strand, within RPSI07_RS13805 at 1285.416 kb on - strand, within RPSI07_RS13805
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,282,770 + -0.8 1,282,770 + +0.3 1,282,771 - -0.5 1,282,771 - -0.2 1,282,793 + -1.3 1,282,794 - +0.2 1,282,794 - +0.2 1,282,794 - +1.2 1,282,807 + -1.0 1,282,808 - -0.5 1,283,097 + RPSI07_RS13795 0.43 +1.0 1,283,534 + +0.3 1,283,535 - -1.0 1,283,535 - -1.2 1,283,535 - +0.5 1,283,535 - -0.3 1,283,541 - +1.4 1,284,119 + RPSI07_RS13800 0.63 -0.8 1,284,119 + RPSI07_RS13800 0.63 +2.2 1,284,120 - RPSI07_RS13800 0.63 +0.4 1,284,120 - RPSI07_RS13800 0.63 -1.2 1,284,120 - RPSI07_RS13800 0.63 -0.6 1,284,158 + RPSI07_RS13800 0.67 +0.9 1,284,159 - RPSI07_RS13800 0.67 +2.7 1,284,260 + RPSI07_RS13800 0.78 -1.0 1,284,260 + RPSI07_RS13800 0.78 +1.3 1,284,261 - RPSI07_RS13800 0.78 +0.0 1,284,440 - +0.7 1,284,452 + +1.2 1,284,453 - -0.4 1,284,453 - -0.3 1,284,536 + -2.2 1,284,536 + -0.3 1,284,537 - -0.1 1,284,574 + -2.3 1,284,575 - +0.2 1,284,702 - RPSI07_RS13805 0.12 -0.7 1,284,938 + RPSI07_RS13805 0.36 -2.1 1,284,939 - RPSI07_RS13805 0.36 +1.7 1,285,020 - RPSI07_RS13805 0.44 -0.1 1,285,020 - RPSI07_RS13805 0.44 -0.4 1,285,106 + RPSI07_RS13805 0.52 +0.2 1,285,106 + RPSI07_RS13805 0.52 +0.2 1,285,107 - RPSI07_RS13805 0.52 +1.0 1,285,107 - RPSI07_RS13805 0.52 +0.8 1,285,107 - RPSI07_RS13805 0.52 -0.4 1,285,108 - RPSI07_RS13805 0.52 +0.2 1,285,265 + RPSI07_RS13805 0.68 +0.3 1,285,269 - RPSI07_RS13805 0.68 -0.4 1,285,271 + RPSI07_RS13805 0.68 +0.0 1,285,271 + RPSI07_RS13805 0.68 +0.2 1,285,271 + RPSI07_RS13805 0.68 -0.6 1,285,271 + RPSI07_RS13805 0.68 -0.8 1,285,272 - RPSI07_RS13805 0.68 +0.2 1,285,272 - RPSI07_RS13805 0.68 +0.2 1,285,272 - RPSI07_RS13805 0.68 +0.5 1,285,272 - RPSI07_RS13805 0.68 -1.7 1,285,415 + RPSI07_RS13805 0.82 +1.2 1,285,416 - RPSI07_RS13805 0.82 +2.2 1,285,416 - RPSI07_RS13805 0.82 -0.6 1,285,416 - RPSI07_RS13805 0.82 -0.4
Or see this region's nucleotide sequence