Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS13595 and RPSI07_RS13600 are separated by 90 nucleotides RPSI07_RS13600 and RPSI07_RS13605 are separated by 183 nucleotides RPSI07_RS13605 and RPSI07_RS13610 are separated by 149 nucleotides
RPSI07_RS13595: RPSI07_RS13595 - pirin family protein, at 1,240,975 to 1,241,673
_RS13595
RPSI07_RS13600: RPSI07_RS13600 - flavodoxin family protein, at 1,241,764 to 1,242,336
_RS13600
RPSI07_RS13605: RPSI07_RS13605 - sulfurtransferase, at 1,242,520 to 1,243,368
_RS13605
RPSI07_RS13610: RPSI07_RS13610 - DNA polymerase III subunit alpha, at 1,243,518 to 1,247,042
_RS13610
Position (kb)
1242
1243
1244 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 1241.533 kb on + strand, within RPSI07_RS13595 at 1241.533 kb on + strand, within RPSI07_RS13595 at 1241.533 kb on + strand, within RPSI07_RS13595 at 1241.785 kb on + strand at 1241.785 kb on + strand at 1241.786 kb on - strand at 1241.947 kb on + strand, within RPSI07_RS13600 at 1241.947 kb on + strand, within RPSI07_RS13600 at 1241.947 kb on + strand at 1241.947 kb on + strand, within RPSI07_RS13600 at 1241.947 kb on + strand, within RPSI07_RS13600 at 1241.948 kb on - strand, within RPSI07_RS13600 at 1241.948 kb on - strand, within RPSI07_RS13600 at 1241.948 kb on - strand, within RPSI07_RS13600 at 1241.948 kb on - strand, within RPSI07_RS13600 at 1242.077 kb on - strand, within RPSI07_RS13600 at 1242.092 kb on - strand, within RPSI07_RS13600 at 1242.092 kb on - strand, within RPSI07_RS13600 at 1242.187 kb on + strand, within RPSI07_RS13600 at 1242.187 kb on + strand, within RPSI07_RS13600 at 1242.187 kb on + strand, within RPSI07_RS13600 at 1242.187 kb on + strand, within RPSI07_RS13600 at 1242.187 kb on + strand, within RPSI07_RS13600 at 1242.187 kb on + strand, within RPSI07_RS13600 at 1242.187 kb on + strand, within RPSI07_RS13600 at 1242.188 kb on - strand, within RPSI07_RS13600 at 1242.188 kb on - strand, within RPSI07_RS13600 at 1242.188 kb on - strand, within RPSI07_RS13600 at 1242.188 kb on - strand, within RPSI07_RS13600 at 1242.188 kb on - strand, within RPSI07_RS13600 at 1242.335 kb on - strand at 1242.578 kb on + strand at 1242.578 kb on + strand at 1242.578 kb on + strand at 1242.579 kb on - strand at 1242.579 kb on - strand at 1242.704 kb on + strand, within RPSI07_RS13605 at 1242.705 kb on - strand, within RPSI07_RS13605 at 1242.732 kb on - strand, within RPSI07_RS13605 at 1242.758 kb on + strand, within RPSI07_RS13605 at 1243.428 kb on - strand at 1243.459 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,241,533 + RPSI07_RS13595 0.80 -0.0 1,241,533 + RPSI07_RS13595 0.80 +1.3 1,241,533 + RPSI07_RS13595 0.80 +0.6 1,241,785 + -2.2 1,241,785 + -0.2 1,241,786 - -0.1 1,241,947 + RPSI07_RS13600 0.32 -0.7 1,241,947 + RPSI07_RS13600 0.32 +0.2 1,241,947 + -0.4 1,241,947 + RPSI07_RS13600 0.32 -1.0 1,241,947 + RPSI07_RS13600 0.32 +0.7 1,241,948 - RPSI07_RS13600 0.32 -0.9 1,241,948 - RPSI07_RS13600 0.32 -1.0 1,241,948 - RPSI07_RS13600 0.32 +0.6 1,241,948 - RPSI07_RS13600 0.32 -0.3 1,242,077 - RPSI07_RS13600 0.55 -1.5 1,242,092 - RPSI07_RS13600 0.57 -0.1 1,242,092 - RPSI07_RS13600 0.57 +0.6 1,242,187 + RPSI07_RS13600 0.74 -0.2 1,242,187 + RPSI07_RS13600 0.74 -1.0 1,242,187 + RPSI07_RS13600 0.74 +0.8 1,242,187 + RPSI07_RS13600 0.74 +0.3 1,242,187 + RPSI07_RS13600 0.74 +2.2 1,242,187 + RPSI07_RS13600 0.74 -0.5 1,242,187 + RPSI07_RS13600 0.74 -1.6 1,242,188 - RPSI07_RS13600 0.74 -0.9 1,242,188 - RPSI07_RS13600 0.74 +7.1 1,242,188 - RPSI07_RS13600 0.74 +0.0 1,242,188 - RPSI07_RS13600 0.74 -0.7 1,242,188 - RPSI07_RS13600 0.74 -0.4 1,242,335 - -2.1 1,242,578 + -0.1 1,242,578 + +1.2 1,242,578 + +1.6 1,242,579 - +0.2 1,242,579 - -0.7 1,242,704 + RPSI07_RS13605 0.22 +0.5 1,242,705 - RPSI07_RS13605 0.22 -2.0 1,242,732 - RPSI07_RS13605 0.25 -0.2 1,242,758 + RPSI07_RS13605 0.28 +1.2 1,243,428 - +1.2 1,243,459 + +0.2
Or see this region's nucleotide sequence