Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS11290 and RPSI07_RS11295 are separated by 55 nucleotides RPSI07_RS11295 and RPSI07_RS11300 overlap by 4 nucleotides RPSI07_RS11300 and RPSI07_RS11305 are separated by 19 nucleotides
RPSI07_RS11290: RPSI07_RS11290 - RNB domain-containing ribonuclease, at 730,897 to 732,978
_RS11290
RPSI07_RS11295: RPSI07_RS11295 - energy transducer TonB, at 733,034 to 733,948
_RS11295
RPSI07_RS11300: RPSI07_RS11300 - shikimate dehydrogenase, at 733,945 to 734,865
_RS11300
RPSI07_RS11305: RPSI07_RS11305 - monofunctional biosynthetic peptidoglycan transglycosylase, at 734,885 to 735,589
_RS11305
Position (kb) 733
734 Strain fitness (log2 ratio) -2
-1
0
1
2 at 732.976 kb on + strand at 732.980 kb on + strand at 732.980 kb on + strand at 732.980 kb on + strand at 732.980 kb on + strand at 732.981 kb on - strand at 733.015 kb on + strand at 733.272 kb on - strand, within RPSI07_RS11295 at 733.544 kb on + strand, within RPSI07_RS11295 at 733.613 kb on + strand, within RPSI07_RS11295 at 733.751 kb on + strand, within RPSI07_RS11295 at 734.005 kb on + strand at 734.092 kb on + strand, within RPSI07_RS11300 at 734.092 kb on + strand, within RPSI07_RS11300 at 734.093 kb on - strand, within RPSI07_RS11300 at 734.093 kb on - strand, within RPSI07_RS11300 at 734.104 kb on + strand, within RPSI07_RS11300 at 734.104 kb on + strand, within RPSI07_RS11300 at 734.104 kb on + strand, within RPSI07_RS11300 at 734.555 kb on + strand at 734.556 kb on - strand, within RPSI07_RS11300 at 734.556 kb on - strand, within RPSI07_RS11300 at 734.556 kb on - strand, within RPSI07_RS11300 at 734.659 kb on + strand, within RPSI07_RS11300 at 734.659 kb on + strand, within RPSI07_RS11300 at 734.660 kb on - strand, within RPSI07_RS11300 at 734.660 kb on - strand, within RPSI07_RS11300 at 734.660 kb on - strand, within RPSI07_RS11300 at 734.660 kb on - strand, within RPSI07_RS11300 at 734.660 kb on - strand, within RPSI07_RS11300 at 734.660 kb on - strand, within RPSI07_RS11300 at 734.904 kb on - strand at 734.948 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 732,976 + +0.6 732,980 + -0.2 732,980 + +0.2 732,980 + -1.3 732,980 + -0.1 732,981 - -1.4 733,015 + +0.1 733,272 - RPSI07_RS11295 0.26 -0.9 733,544 + RPSI07_RS11295 0.56 -1.0 733,613 + RPSI07_RS11295 0.63 +1.9 733,751 + RPSI07_RS11295 0.78 -1.5 734,005 + +0.0 734,092 + RPSI07_RS11300 0.16 -0.4 734,092 + RPSI07_RS11300 0.16 -0.3 734,093 - RPSI07_RS11300 0.16 -0.3 734,093 - RPSI07_RS11300 0.16 +1.6 734,104 + RPSI07_RS11300 0.17 -2.2 734,104 + RPSI07_RS11300 0.17 -0.8 734,104 + RPSI07_RS11300 0.17 +0.1 734,555 + +1.9 734,556 - RPSI07_RS11300 0.66 -1.1 734,556 - RPSI07_RS11300 0.66 -1.0 734,556 - RPSI07_RS11300 0.66 -1.4 734,659 + RPSI07_RS11300 0.78 -0.3 734,659 + RPSI07_RS11300 0.78 -0.3 734,660 - RPSI07_RS11300 0.78 +0.6 734,660 - RPSI07_RS11300 0.78 +2.0 734,660 - RPSI07_RS11300 0.78 +1.0 734,660 - RPSI07_RS11300 0.78 -1.3 734,660 - RPSI07_RS11300 0.78 +0.9 734,660 - RPSI07_RS11300 0.78 -0.5 734,904 - -0.8 734,948 + +0.0
Or see this region's nucleotide sequence