Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10615 and RPSI07_RS10620 are separated by 312 nucleotides RPSI07_RS10620 and RPSI07_RS10625 are separated by 19 nucleotides RPSI07_RS10625 and RPSI07_RS10630 are separated by 145 nucleotides
RPSI07_RS10615: RPSI07_RS10615 - hypothetical protein, at 585,468 to 586,196
_RS10615
RPSI07_RS10620: RPSI07_RS10620 - UbiX family flavin prenyltransferase, at 586,509 to 587,165
_RS10620
RPSI07_RS10625: RPSI07_RS10625 - monothiol glutaredoxin, Grx4 family, at 587,185 to 587,496
_RS10625
RPSI07_RS10630: RPSI07_RS10630 - group II intron reverse transcriptase/maturase, at 587,642 to 589,339
_RS10630
Position (kb) 587
588 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 586.201 kb on - strand at 586.217 kb on - strand at 586.227 kb on + strand at 586.228 kb on - strand at 586.228 kb on - strand at 586.236 kb on + strand at 586.237 kb on - strand at 586.272 kb on + strand at 586.273 kb on - strand at 586.273 kb on - strand at 586.275 kb on - strand at 586.372 kb on + strand at 586.408 kb on + strand at 586.409 kb on - strand at 586.429 kb on + strand at 586.432 kb on + strand at 586.433 kb on - strand at 586.461 kb on + strand at 586.461 kb on + strand at 586.461 kb on + strand at 586.462 kb on - strand at 586.465 kb on + strand at 586.469 kb on - strand at 587.223 kb on - strand, within RPSI07_RS10625 at 587.223 kb on - strand, within RPSI07_RS10625 at 587.587 kb on - strand at 587.587 kb on - strand at 587.595 kb on - strand at 587.595 kb on - strand at 587.595 kb on - strand at 587.595 kb on - strand at 587.615 kb on - strand at 587.630 kb on - strand at 587.644 kb on - strand at 587.644 kb on - strand at 587.644 kb on - strand at 587.724 kb on - strand at 587.724 kb on - strand at 587.744 kb on + strand at 587.745 kb on - strand at 587.750 kb on + strand at 587.832 kb on + strand, within RPSI07_RS10630 at 587.833 kb on - strand, within RPSI07_RS10630 at 587.833 kb on - strand, within RPSI07_RS10630 at 587.839 kb on + strand, within RPSI07_RS10630 at 587.839 kb on + strand, within RPSI07_RS10630 at 587.931 kb on + strand, within RPSI07_RS10630 at 587.931 kb on + strand, within RPSI07_RS10630 at 587.931 kb on + strand, within RPSI07_RS10630 at 587.931 kb on + strand, within RPSI07_RS10630 at 587.932 kb on - strand, within RPSI07_RS10630 at 587.982 kb on + strand, within RPSI07_RS10630 at 588.066 kb on + strand, within RPSI07_RS10630 at 588.067 kb on - strand, within RPSI07_RS10630 at 588.140 kb on - strand, within RPSI07_RS10630 at 588.189 kb on + strand, within RPSI07_RS10630 at 588.254 kb on - strand, within RPSI07_RS10630 at 588.336 kb on + strand, within RPSI07_RS10630 at 588.337 kb on - strand, within RPSI07_RS10630
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 586,201 - +0.3 586,217 - -0.5 586,227 + +0.8 586,228 - +0.6 586,228 - +0.1 586,236 + +0.3 586,237 - -0.5 586,272 + +0.3 586,273 - +3.2 586,273 - -0.0 586,275 - -0.2 586,372 + +0.5 586,408 + -0.3 586,409 - -1.0 586,429 + -1.1 586,432 + +1.3 586,433 - +0.4 586,461 + +0.3 586,461 + +1.3 586,461 + +1.7 586,462 - -0.7 586,465 + -0.2 586,469 - +1.6 587,223 - RPSI07_RS10625 0.12 -1.3 587,223 - RPSI07_RS10625 0.12 +2.3 587,587 - +1.3 587,587 - -0.9 587,595 - -1.1 587,595 - -0.4 587,595 - -1.4 587,595 - -0.6 587,615 - +1.3 587,630 - +0.3 587,644 - +0.0 587,644 - -0.3 587,644 - +0.0 587,724 - -0.4 587,724 - +0.1 587,744 + +0.5 587,745 - -0.3 587,750 + +0.2 587,832 + RPSI07_RS10630 0.11 +0.6 587,833 - RPSI07_RS10630 0.11 +0.3 587,833 - RPSI07_RS10630 0.11 -0.5 587,839 + RPSI07_RS10630 0.12 -0.2 587,839 + RPSI07_RS10630 0.12 +1.3 587,931 + RPSI07_RS10630 0.17 -0.5 587,931 + RPSI07_RS10630 0.17 -1.3 587,931 + RPSI07_RS10630 0.17 -0.3 587,931 + RPSI07_RS10630 0.17 -0.1 587,932 - RPSI07_RS10630 0.17 +0.3 587,982 + RPSI07_RS10630 0.20 +0.8 588,066 + RPSI07_RS10630 0.25 -0.3 588,067 - RPSI07_RS10630 0.25 +2.1 588,140 - RPSI07_RS10630 0.29 -0.1 588,189 + RPSI07_RS10630 0.32 +0.3 588,254 - RPSI07_RS10630 0.36 -0.0 588,336 + RPSI07_RS10630 0.41 +1.9 588,337 - RPSI07_RS10630 0.41 -2.1
Or see this region's nucleotide sequence