Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10480 and RPSI07_RS10485 are separated by 336 nucleotides RPSI07_RS10485 and RPSI07_RS10490 are separated by 418 nucleotides
RPSI07_RS10480: RPSI07_RS10480 - iron ABC transporter substrate-binding protein, at 553,254 to 554,396
_RS10480
RPSI07_RS10485: RPSI07_RS10485 - porin, at 554,733 to 555,860
_RS10485
RPSI07_RS10490: RPSI07_RS10490 - PDZ domain-containing protein, at 556,279 to 557,493
_RS10490
Position (kb) 554
555
556 Strain fitness (log2 ratio) -2
-1
0
1 at 553.753 kb on - strand, within RPSI07_RS10480 at 553.857 kb on + strand, within RPSI07_RS10480 at 553.857 kb on + strand, within RPSI07_RS10480 at 553.858 kb on - strand, within RPSI07_RS10480 at 553.858 kb on - strand, within RPSI07_RS10480 at 553.858 kb on - strand, within RPSI07_RS10480 at 553.911 kb on - strand, within RPSI07_RS10480 at 553.911 kb on - strand, within RPSI07_RS10480 at 553.968 kb on + strand, within RPSI07_RS10480 at 553.968 kb on + strand, within RPSI07_RS10480 at 553.968 kb on + strand, within RPSI07_RS10480 at 553.968 kb on + strand, within RPSI07_RS10480 at 553.969 kb on - strand, within RPSI07_RS10480 at 553.970 kb on + strand, within RPSI07_RS10480 at 553.970 kb on + strand, within RPSI07_RS10480 at 553.970 kb on + strand, within RPSI07_RS10480 at 553.971 kb on - strand, within RPSI07_RS10480 at 554.026 kb on - strand, within RPSI07_RS10480 at 554.028 kb on - strand, within RPSI07_RS10480 at 554.028 kb on - strand, within RPSI07_RS10480 at 554.028 kb on - strand, within RPSI07_RS10480 at 554.055 kb on + strand, within RPSI07_RS10480 at 554.055 kb on + strand, within RPSI07_RS10480 at 554.100 kb on + strand, within RPSI07_RS10480 at 554.295 kb on - strand at 554.295 kb on - strand at 554.366 kb on + strand at 554.420 kb on + strand at 554.424 kb on + strand at 555.978 kb on - strand at 555.986 kb on - strand at 556.281 kb on - strand at 556.727 kb on + strand, within RPSI07_RS10490 at 556.727 kb on + strand, within RPSI07_RS10490 at 556.834 kb on - strand, within RPSI07_RS10490 at 556.834 kb on - strand, within RPSI07_RS10490
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 553,753 - RPSI07_RS10480 0.44 -0.5 553,857 + RPSI07_RS10480 0.53 +0.3 553,857 + RPSI07_RS10480 0.53 +1.3 553,858 - RPSI07_RS10480 0.53 -0.3 553,858 - RPSI07_RS10480 0.53 +0.4 553,858 - RPSI07_RS10480 0.53 -0.3 553,911 - RPSI07_RS10480 0.57 +0.3 553,911 - RPSI07_RS10480 0.57 +1.0 553,968 + RPSI07_RS10480 0.62 -0.7 553,968 + RPSI07_RS10480 0.62 +0.9 553,968 + RPSI07_RS10480 0.62 +0.3 553,968 + RPSI07_RS10480 0.62 +1.6 553,969 - RPSI07_RS10480 0.63 -2.0 553,970 + RPSI07_RS10480 0.63 +1.3 553,970 + RPSI07_RS10480 0.63 +0.8 553,970 + RPSI07_RS10480 0.63 +0.6 553,971 - RPSI07_RS10480 0.63 -1.3 554,026 - RPSI07_RS10480 0.68 -1.2 554,028 - RPSI07_RS10480 0.68 -1.2 554,028 - RPSI07_RS10480 0.68 +0.2 554,028 - RPSI07_RS10480 0.68 -0.7 554,055 + RPSI07_RS10480 0.70 +0.0 554,055 + RPSI07_RS10480 0.70 +0.7 554,100 + RPSI07_RS10480 0.74 -1.8 554,295 - -1.5 554,295 - -0.2 554,366 + +0.4 554,420 + +1.0 554,424 + +0.7 555,978 - -0.5 555,986 - +0.4 556,281 - -1.7 556,727 + RPSI07_RS10490 0.37 -1.1 556,727 + RPSI07_RS10490 0.37 -2.4 556,834 - RPSI07_RS10490 0.46 +0.1 556,834 - RPSI07_RS10490 0.46 -0.7
Or see this region's nucleotide sequence