Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS07810 and RPSI07_RS07815 are separated by 371 nucleotides RPSI07_RS07815 and RPSI07_RS07820 are separated by 112 nucleotides
RPSI07_RS07810: RPSI07_RS07810 - peptide chain release factor 3, at 2,065,805 to 2,067,400
_RS07810
RPSI07_RS07815: RPSI07_RS07815 - hypothetical protein, at 2,067,772 to 2,067,999
_RS07815
RPSI07_RS07820: RPSI07_RS07820 - YihY/virulence factor BrkB family protein, at 2,068,112 to 2,069,083
_RS07820
Position (kb)
2067
2068 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2066.847 kb on + strand, within RPSI07_RS07810 at 2066.847 kb on + strand, within RPSI07_RS07810 at 2066.848 kb on - strand, within RPSI07_RS07810 at 2066.848 kb on - strand, within RPSI07_RS07810 at 2066.848 kb on - strand, within RPSI07_RS07810 at 2066.848 kb on - strand, within RPSI07_RS07810 at 2067.096 kb on + strand, within RPSI07_RS07810 at 2067.150 kb on + strand, within RPSI07_RS07810 at 2067.150 kb on + strand, within RPSI07_RS07810 at 2067.150 kb on + strand, within RPSI07_RS07810 at 2067.150 kb on + strand, within RPSI07_RS07810 at 2067.151 kb on - strand, within RPSI07_RS07810 at 2067.151 kb on - strand, within RPSI07_RS07810 at 2067.151 kb on - strand, within RPSI07_RS07810 at 2067.394 kb on + strand at 2067.395 kb on - strand at 2067.457 kb on - strand at 2067.525 kb on + strand at 2067.525 kb on + strand at 2067.526 kb on - strand at 2067.526 kb on - strand at 2067.617 kb on + strand at 2067.617 kb on + strand at 2067.617 kb on + strand at 2067.617 kb on + strand at 2067.617 kb on + strand at 2067.617 kb on + strand at 2067.618 kb on - strand at 2067.618 kb on - strand at 2067.618 kb on - strand at 2067.619 kb on - strand at 2067.778 kb on - strand at 2068.032 kb on + strand at 2068.032 kb on + strand at 2068.032 kb on + strand at 2068.032 kb on + strand at 2068.032 kb on + strand at 2068.033 kb on - strand at 2068.033 kb on - strand at 2068.033 kb on - strand at 2068.033 kb on - strand at 2068.230 kb on + strand, within RPSI07_RS07820 at 2068.231 kb on - strand, within RPSI07_RS07820 at 2068.231 kb on - strand, within RPSI07_RS07820 at 2068.231 kb on - strand, within RPSI07_RS07820 at 2068.231 kb on - strand, within RPSI07_RS07820 at 2068.231 kb on - strand, within RPSI07_RS07820 at 2068.257 kb on + strand, within RPSI07_RS07820 at 2068.278 kb on + strand, within RPSI07_RS07820 at 2068.278 kb on + strand, within RPSI07_RS07820 at 2068.278 kb on + strand, within RPSI07_RS07820 at 2068.278 kb on + strand, within RPSI07_RS07820 at 2068.278 kb on + strand, within RPSI07_RS07820 at 2068.279 kb on - strand, within RPSI07_RS07820 at 2068.279 kb on - strand, within RPSI07_RS07820 at 2068.279 kb on - strand, within RPSI07_RS07820 at 2068.279 kb on - strand, within RPSI07_RS07820 at 2068.279 kb on - strand, within RPSI07_RS07820 at 2068.279 kb on - strand, within RPSI07_RS07820 at 2068.279 kb on - strand, within RPSI07_RS07820 at 2068.321 kb on - strand, within RPSI07_RS07820 at 2068.321 kb on - strand, within RPSI07_RS07820 at 2068.350 kb on + strand, within RPSI07_RS07820 at 2068.350 kb on + strand, within RPSI07_RS07820 at 2068.350 kb on + strand, within RPSI07_RS07820 at 2068.351 kb on - strand, within RPSI07_RS07820 at 2068.351 kb on - strand, within RPSI07_RS07820 at 2068.959 kb on + strand, within RPSI07_RS07820 at 2068.960 kb on - strand, within RPSI07_RS07820 at 2068.960 kb on - strand, within RPSI07_RS07820
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 2,066,847 + RPSI07_RS07810 0.65 -0.8 2,066,847 + RPSI07_RS07810 0.65 +1.5 2,066,848 - RPSI07_RS07810 0.65 +2.6 2,066,848 - RPSI07_RS07810 0.65 -0.8 2,066,848 - RPSI07_RS07810 0.65 +0.6 2,066,848 - RPSI07_RS07810 0.65 -0.6 2,067,096 + RPSI07_RS07810 0.81 +0.4 2,067,150 + RPSI07_RS07810 0.84 +0.0 2,067,150 + RPSI07_RS07810 0.84 -0.2 2,067,150 + RPSI07_RS07810 0.84 -1.8 2,067,150 + RPSI07_RS07810 0.84 +1.0 2,067,151 - RPSI07_RS07810 0.84 +1.4 2,067,151 - RPSI07_RS07810 0.84 +3.6 2,067,151 - RPSI07_RS07810 0.84 -0.8 2,067,394 + +1.0 2,067,395 - -0.0 2,067,457 - +4.4 2,067,525 + +2.0 2,067,525 + +2.0 2,067,526 - +0.8 2,067,526 - +0.0 2,067,617 + +0.2 2,067,617 + +0.7 2,067,617 + -1.8 2,067,617 + +0.8 2,067,617 + +0.4 2,067,617 + -1.6 2,067,618 - +0.4 2,067,618 - -0.3 2,067,618 - -1.6 2,067,619 - +1.3 2,067,778 - -1.3 2,068,032 + -0.1 2,068,032 + +2.6 2,068,032 + -0.0 2,068,032 + -2.1 2,068,032 + -2.0 2,068,033 - -0.2 2,068,033 - +1.0 2,068,033 - +0.5 2,068,033 - -3.0 2,068,230 + RPSI07_RS07820 0.12 +0.6 2,068,231 - RPSI07_RS07820 0.12 -0.4 2,068,231 - RPSI07_RS07820 0.12 +0.2 2,068,231 - RPSI07_RS07820 0.12 -0.7 2,068,231 - RPSI07_RS07820 0.12 +1.5 2,068,231 - RPSI07_RS07820 0.12 +0.9 2,068,257 + RPSI07_RS07820 0.15 -0.3 2,068,278 + RPSI07_RS07820 0.17 +0.9 2,068,278 + RPSI07_RS07820 0.17 -1.1 2,068,278 + RPSI07_RS07820 0.17 +0.0 2,068,278 + RPSI07_RS07820 0.17 -0.6 2,068,278 + RPSI07_RS07820 0.17 +0.7 2,068,279 - RPSI07_RS07820 0.17 -1.0 2,068,279 - RPSI07_RS07820 0.17 +0.0 2,068,279 - RPSI07_RS07820 0.17 +0.6 2,068,279 - RPSI07_RS07820 0.17 +0.7 2,068,279 - RPSI07_RS07820 0.17 +1.7 2,068,279 - RPSI07_RS07820 0.17 +1.3 2,068,279 - RPSI07_RS07820 0.17 -0.8 2,068,321 - RPSI07_RS07820 0.22 +0.3 2,068,321 - RPSI07_RS07820 0.22 -1.8 2,068,350 + RPSI07_RS07820 0.24 +1.4 2,068,350 + RPSI07_RS07820 0.24 +0.2 2,068,350 + RPSI07_RS07820 0.24 +0.6 2,068,351 - RPSI07_RS07820 0.25 -1.1 2,068,351 - RPSI07_RS07820 0.25 +1.3 2,068,959 + RPSI07_RS07820 0.87 +0.4 2,068,960 - RPSI07_RS07820 0.87 +0.2 2,068,960 - RPSI07_RS07820 0.87 +1.0
Or see this region's nucleotide sequence