Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS07795 and RPSI07_RS07800 are separated by 96 nucleotides RPSI07_RS07800 and RPSI07_RS07805 are separated by 45 nucleotides
RPSI07_RS07795: RPSI07_RS07795 - acyl-CoA desaturase, at 2,062,855 to 2,063,781
_RS07795
RPSI07_RS07800: RPSI07_RS07800 - patatin, at 2,063,878 to 2,064,741
_RS07800
RPSI07_RS07805: RPSI07_RS07805 - alpha/beta hydrolase, at 2,064,787 to 2,065,680
_RS07805
Position (kb)
2063
2064
2065 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2062.996 kb on + strand, within RPSI07_RS07795 at 2062.996 kb on + strand, within RPSI07_RS07795 at 2062.997 kb on - strand, within RPSI07_RS07795 at 2063.212 kb on + strand, within RPSI07_RS07795 at 2063.212 kb on + strand, within RPSI07_RS07795 at 2063.279 kb on + strand, within RPSI07_RS07795 at 2063.320 kb on + strand, within RPSI07_RS07795 at 2063.321 kb on - strand, within RPSI07_RS07795 at 2063.368 kb on + strand, within RPSI07_RS07795 at 2063.599 kb on + strand, within RPSI07_RS07795 at 2063.599 kb on + strand, within RPSI07_RS07795 at 2063.601 kb on + strand, within RPSI07_RS07795 at 2063.601 kb on + strand, within RPSI07_RS07795 at 2063.601 kb on + strand, within RPSI07_RS07795 at 2063.626 kb on + strand, within RPSI07_RS07795 at 2063.626 kb on + strand, within RPSI07_RS07795 at 2064.979 kb on + strand, within RPSI07_RS07805 at 2064.979 kb on + strand, within RPSI07_RS07805 at 2065.135 kb on + strand, within RPSI07_RS07805 at 2065.205 kb on - strand, within RPSI07_RS07805 at 2065.291 kb on + strand, within RPSI07_RS07805 at 2065.291 kb on + strand, within RPSI07_RS07805 at 2065.291 kb on + strand, within RPSI07_RS07805 at 2065.292 kb on - strand, within RPSI07_RS07805 at 2065.292 kb on - strand, within RPSI07_RS07805 at 2065.292 kb on - strand, within RPSI07_RS07805 at 2065.292 kb on - strand, within RPSI07_RS07805 at 2065.386 kb on - strand, within RPSI07_RS07805 at 2065.386 kb on - strand, within RPSI07_RS07805 at 2065.386 kb on - strand, within RPSI07_RS07805 at 2065.393 kb on - strand, within RPSI07_RS07805
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 2,062,996 + RPSI07_RS07795 0.15 -1.4 2,062,996 + RPSI07_RS07795 0.15 -1.3 2,062,997 - RPSI07_RS07795 0.15 -1.0 2,063,212 + RPSI07_RS07795 0.39 +1.8 2,063,212 + RPSI07_RS07795 0.39 +2.8 2,063,279 + RPSI07_RS07795 0.46 +0.2 2,063,320 + RPSI07_RS07795 0.50 +2.6 2,063,321 - RPSI07_RS07795 0.50 +1.0 2,063,368 + RPSI07_RS07795 0.55 -0.6 2,063,599 + RPSI07_RS07795 0.80 -0.7 2,063,599 + RPSI07_RS07795 0.80 +1.6 2,063,601 + RPSI07_RS07795 0.80 -0.4 2,063,601 + RPSI07_RS07795 0.80 +0.2 2,063,601 + RPSI07_RS07795 0.80 +0.4 2,063,626 + RPSI07_RS07795 0.83 -0.8 2,063,626 + RPSI07_RS07795 0.83 +0.7 2,064,979 + RPSI07_RS07805 0.21 -2.1 2,064,979 + RPSI07_RS07805 0.21 -1.0 2,065,135 + RPSI07_RS07805 0.39 -2.0 2,065,205 - RPSI07_RS07805 0.47 -2.0 2,065,291 + RPSI07_RS07805 0.56 -1.6 2,065,291 + RPSI07_RS07805 0.56 -1.8 2,065,291 + RPSI07_RS07805 0.56 -1.3 2,065,292 - RPSI07_RS07805 0.56 -2.0 2,065,292 - RPSI07_RS07805 0.56 -1.0 2,065,292 - RPSI07_RS07805 0.56 -2.0 2,065,292 - RPSI07_RS07805 0.56 -1.8 2,065,386 - RPSI07_RS07805 0.67 -1.5 2,065,386 - RPSI07_RS07805 0.67 -0.2 2,065,386 - RPSI07_RS07805 0.67 -1.3 2,065,393 - RPSI07_RS07805 0.68 +0.3
Or see this region's nucleotide sequence