Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS07780 and RPSI07_RS07785 overlap by 4 nucleotides RPSI07_RS07785 and RPSI07_RS07790 are separated by 31 nucleotides RPSI07_RS07790 and RPSI07_RS07795 overlap by 4 nucleotides RPSI07_RS07795 and RPSI07_RS07800 are separated by 96 nucleotides
RPSI07_RS07780: RPSI07_RS07780 - class I SAM-dependent methyltransferase, at 2,061,263 to 2,062,135
_RS07780
RPSI07_RS07785: RPSI07_RS07785 - thioesterase, at 2,062,132 to 2,062,563
_RS07785
RPSI07_RS07790: RPSI07_RS07790 - acyl carrier protein, at 2,062,595 to 2,062,858
_RS07790
RPSI07_RS07795: RPSI07_RS07795 - acyl-CoA desaturase, at 2,062,855 to 2,063,781
_RS07795
RPSI07_RS07800: RPSI07_RS07800 - patatin, at 2,063,878 to 2,064,741
_RS07800
Position (kb)
2062
2063
2064 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2061.902 kb on + strand, within RPSI07_RS07780 at 2061.902 kb on + strand, within RPSI07_RS07780 at 2062.019 kb on + strand, within RPSI07_RS07780 at 2062.019 kb on + strand, within RPSI07_RS07780 at 2062.019 kb on + strand, within RPSI07_RS07780 at 2062.028 kb on + strand, within RPSI07_RS07780 at 2062.028 kb on + strand, within RPSI07_RS07780 at 2062.028 kb on + strand, within RPSI07_RS07780 at 2062.028 kb on + strand, within RPSI07_RS07780 at 2062.028 kb on + strand, within RPSI07_RS07780 at 2062.028 kb on + strand, within RPSI07_RS07780 at 2062.037 kb on + strand, within RPSI07_RS07780 at 2062.037 kb on + strand, within RPSI07_RS07780 at 2062.037 kb on + strand, within RPSI07_RS07780 at 2062.037 kb on + strand, within RPSI07_RS07780 at 2062.814 kb on + strand at 2062.996 kb on + strand, within RPSI07_RS07795 at 2062.996 kb on + strand, within RPSI07_RS07795 at 2062.997 kb on - strand, within RPSI07_RS07795 at 2063.212 kb on + strand, within RPSI07_RS07795 at 2063.212 kb on + strand, within RPSI07_RS07795 at 2063.279 kb on + strand, within RPSI07_RS07795 at 2063.320 kb on + strand, within RPSI07_RS07795 at 2063.321 kb on - strand, within RPSI07_RS07795 at 2063.368 kb on + strand, within RPSI07_RS07795 at 2063.599 kb on + strand, within RPSI07_RS07795 at 2063.599 kb on + strand, within RPSI07_RS07795 at 2063.601 kb on + strand, within RPSI07_RS07795 at 2063.601 kb on + strand, within RPSI07_RS07795 at 2063.601 kb on + strand, within RPSI07_RS07795 at 2063.626 kb on + strand, within RPSI07_RS07795 at 2063.626 kb on + strand, within RPSI07_RS07795
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 2,061,902 + RPSI07_RS07780 0.73 -0.6 2,061,902 + RPSI07_RS07780 0.73 +1.6 2,062,019 + RPSI07_RS07780 0.87 +0.4 2,062,019 + RPSI07_RS07780 0.87 -1.0 2,062,019 + RPSI07_RS07780 0.87 +1.5 2,062,028 + RPSI07_RS07780 0.88 -0.8 2,062,028 + RPSI07_RS07780 0.88 -0.6 2,062,028 + RPSI07_RS07780 0.88 -1.9 2,062,028 + RPSI07_RS07780 0.88 -1.2 2,062,028 + RPSI07_RS07780 0.88 -0.9 2,062,028 + RPSI07_RS07780 0.88 -0.1 2,062,037 + RPSI07_RS07780 0.89 -0.1 2,062,037 + RPSI07_RS07780 0.89 -0.6 2,062,037 + RPSI07_RS07780 0.89 +1.4 2,062,037 + RPSI07_RS07780 0.89 +0.7 2,062,814 + +0.0 2,062,996 + RPSI07_RS07795 0.15 -1.4 2,062,996 + RPSI07_RS07795 0.15 -1.3 2,062,997 - RPSI07_RS07795 0.15 -1.0 2,063,212 + RPSI07_RS07795 0.39 +1.8 2,063,212 + RPSI07_RS07795 0.39 +2.8 2,063,279 + RPSI07_RS07795 0.46 +0.2 2,063,320 + RPSI07_RS07795 0.50 +2.6 2,063,321 - RPSI07_RS07795 0.50 +1.0 2,063,368 + RPSI07_RS07795 0.55 -0.6 2,063,599 + RPSI07_RS07795 0.80 -0.7 2,063,599 + RPSI07_RS07795 0.80 +1.6 2,063,601 + RPSI07_RS07795 0.80 -0.4 2,063,601 + RPSI07_RS07795 0.80 +0.2 2,063,601 + RPSI07_RS07795 0.80 +0.4 2,063,626 + RPSI07_RS07795 0.83 -0.8 2,063,626 + RPSI07_RS07795 0.83 +0.7
Or see this region's nucleotide sequence