Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS05150 and RPSI07_RS05155 are separated by 123 nucleotides RPSI07_RS05155 and RPSI07_RS05160 are separated by 97 nucleotides RPSI07_RS05160 and RPSI07_RS05165 are separated by 282 nucleotides
RPSI07_RS05150: RPSI07_RS05150 - alpha/beta hydrolase, at 1,407,220 to 1,408,239
_RS05150
RPSI07_RS05155: RPSI07_RS05155 - hypothetical protein, at 1,408,363 to 1,409,130
_RS05155
RPSI07_RS05160: RPSI07_RS05160 - LysR family transcriptional regulator, at 1,409,228 to 1,410,172
_RS05160
RPSI07_RS05165: RPSI07_RS05165 - alpha/beta hydrolase, at 1,410,455 to 1,411,546
_RS05165
Position (kb)
1409
1410
1411 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1408.484 kb on + strand, within RPSI07_RS05155 at 1408.485 kb on - strand, within RPSI07_RS05155 at 1408.485 kb on - strand, within RPSI07_RS05155 at 1408.498 kb on + strand, within RPSI07_RS05155 at 1408.498 kb on + strand, within RPSI07_RS05155 at 1408.498 kb on + strand, within RPSI07_RS05155 at 1408.498 kb on + strand, within RPSI07_RS05155 at 1408.499 kb on - strand, within RPSI07_RS05155 at 1408.499 kb on - strand, within RPSI07_RS05155 at 1408.499 kb on - strand, within RPSI07_RS05155 at 1408.499 kb on - strand, within RPSI07_RS05155 at 1408.499 kb on - strand, within RPSI07_RS05155 at 1408.499 kb on - strand, within RPSI07_RS05155 at 1408.509 kb on + strand, within RPSI07_RS05155 at 1408.509 kb on + strand, within RPSI07_RS05155 at 1408.793 kb on + strand, within RPSI07_RS05155 at 1408.794 kb on - strand, within RPSI07_RS05155 at 1408.794 kb on - strand, within RPSI07_RS05155 at 1408.806 kb on - strand, within RPSI07_RS05155 at 1409.142 kb on + strand at 1409.355 kb on + strand, within RPSI07_RS05160 at 1409.356 kb on - strand, within RPSI07_RS05160 at 1409.389 kb on - strand, within RPSI07_RS05160 at 1409.389 kb on - strand, within RPSI07_RS05160 at 1409.389 kb on - strand, within RPSI07_RS05160 at 1409.433 kb on + strand, within RPSI07_RS05160 at 1409.433 kb on + strand, within RPSI07_RS05160 at 1409.434 kb on - strand, within RPSI07_RS05160 at 1409.434 kb on - strand, within RPSI07_RS05160 at 1409.434 kb on - strand, within RPSI07_RS05160 at 1409.434 kb on - strand, within RPSI07_RS05160 at 1409.598 kb on + strand, within RPSI07_RS05160 at 1409.598 kb on + strand, within RPSI07_RS05160 at 1409.599 kb on - strand, within RPSI07_RS05160 at 1409.599 kb on - strand, within RPSI07_RS05160 at 1409.662 kb on - strand, within RPSI07_RS05160 at 1409.820 kb on + strand, within RPSI07_RS05160 at 1409.821 kb on - strand, within RPSI07_RS05160 at 1409.976 kb on + strand, within RPSI07_RS05160 at 1409.977 kb on - strand, within RPSI07_RS05160 at 1409.977 kb on - strand, within RPSI07_RS05160 at 1409.977 kb on - strand, within RPSI07_RS05160 at 1409.979 kb on + strand, within RPSI07_RS05160 at 1410.308 kb on - strand at 1410.308 kb on - strand at 1410.350 kb on + strand at 1410.353 kb on + strand at 1410.353 kb on + strand at 1410.353 kb on + strand at 1410.354 kb on - strand at 1410.354 kb on - strand at 1410.354 kb on - strand at 1410.354 kb on - strand at 1410.354 kb on - strand at 1410.354 kb on - strand at 1410.368 kb on - strand at 1410.369 kb on + strand at 1410.369 kb on + strand at 1410.369 kb on + strand at 1410.370 kb on - strand at 1410.370 kb on - strand at 1410.378 kb on - strand at 1410.378 kb on - strand at 1410.391 kb on + strand at 1410.437 kb on + strand at 1410.680 kb on + strand, within RPSI07_RS05165 at 1410.782 kb on + strand, within RPSI07_RS05165 at 1410.782 kb on + strand, within RPSI07_RS05165 at 1410.783 kb on - strand, within RPSI07_RS05165 at 1410.783 kb on - strand, within RPSI07_RS05165 at 1410.783 kb on - strand, within RPSI07_RS05165 at 1411.087 kb on - strand, within RPSI07_RS05165 at 1411.087 kb on - strand, within RPSI07_RS05165 at 1411.126 kb on - strand, within RPSI07_RS05165
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 1,408,484 + RPSI07_RS05155 0.16 -0.8 1,408,485 - RPSI07_RS05155 0.16 -1.2 1,408,485 - RPSI07_RS05155 0.16 +0.3 1,408,498 + RPSI07_RS05155 0.18 -0.4 1,408,498 + RPSI07_RS05155 0.18 -0.4 1,408,498 + RPSI07_RS05155 0.18 +0.6 1,408,498 + RPSI07_RS05155 0.18 +0.3 1,408,499 - RPSI07_RS05155 0.18 -0.9 1,408,499 - RPSI07_RS05155 0.18 -0.4 1,408,499 - RPSI07_RS05155 0.18 +1.0 1,408,499 - RPSI07_RS05155 0.18 -1.1 1,408,499 - RPSI07_RS05155 0.18 +0.1 1,408,499 - RPSI07_RS05155 0.18 -1.5 1,408,509 + RPSI07_RS05155 0.19 -0.8 1,408,509 + RPSI07_RS05155 0.19 -0.3 1,408,793 + RPSI07_RS05155 0.56 -2.4 1,408,794 - RPSI07_RS05155 0.56 +0.2 1,408,794 - RPSI07_RS05155 0.56 +0.0 1,408,806 - RPSI07_RS05155 0.58 +0.5 1,409,142 + -0.6 1,409,355 + RPSI07_RS05160 0.13 -1.5 1,409,356 - RPSI07_RS05160 0.14 -1.5 1,409,389 - RPSI07_RS05160 0.17 -0.9 1,409,389 - RPSI07_RS05160 0.17 -0.5 1,409,389 - RPSI07_RS05160 0.17 -0.1 1,409,433 + RPSI07_RS05160 0.22 +0.1 1,409,433 + RPSI07_RS05160 0.22 +2.8 1,409,434 - RPSI07_RS05160 0.22 -2.7 1,409,434 - RPSI07_RS05160 0.22 +0.6 1,409,434 - RPSI07_RS05160 0.22 -0.5 1,409,434 - RPSI07_RS05160 0.22 -0.5 1,409,598 + RPSI07_RS05160 0.39 -0.1 1,409,598 + RPSI07_RS05160 0.39 -1.9 1,409,599 - RPSI07_RS05160 0.39 -2.3 1,409,599 - RPSI07_RS05160 0.39 -1.3 1,409,662 - RPSI07_RS05160 0.46 +0.1 1,409,820 + RPSI07_RS05160 0.63 -1.8 1,409,821 - RPSI07_RS05160 0.63 +1.5 1,409,976 + RPSI07_RS05160 0.79 -1.1 1,409,977 - RPSI07_RS05160 0.79 +0.5 1,409,977 - RPSI07_RS05160 0.79 -0.9 1,409,977 - RPSI07_RS05160 0.79 +0.8 1,409,979 + RPSI07_RS05160 0.79 -0.5 1,410,308 - -1.2 1,410,308 - -1.9 1,410,350 + +1.4 1,410,353 + +2.1 1,410,353 + -0.7 1,410,353 + +1.4 1,410,354 - -0.4 1,410,354 - -0.4 1,410,354 - +1.6 1,410,354 - +1.6 1,410,354 - -1.4 1,410,354 - -0.3 1,410,368 - +0.4 1,410,369 + -0.3 1,410,369 + +0.6 1,410,369 + -1.8 1,410,370 - +1.3 1,410,370 - -0.9 1,410,378 - -1.5 1,410,378 - -1.3 1,410,391 + -0.4 1,410,437 + +1.1 1,410,680 + RPSI07_RS05165 0.21 -1.1 1,410,782 + RPSI07_RS05165 0.30 +0.3 1,410,782 + RPSI07_RS05165 0.30 +0.3 1,410,783 - RPSI07_RS05165 0.30 -0.1 1,410,783 - RPSI07_RS05165 0.30 -1.4 1,410,783 - RPSI07_RS05165 0.30 +1.9 1,411,087 - RPSI07_RS05165 0.58 +0.5 1,411,087 - RPSI07_RS05165 0.58 +2.1 1,411,126 - RPSI07_RS05165 0.61 -1.3
Or see this region's nucleotide sequence