Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS22490 and RPSI07_RS22495 are separated by 46 nucleotides RPSI07_RS22495 and RPSI07_RS22500 are separated by 63 nucleotides RPSI07_RS22500 and RPSI07_RS22505 are separated by 81 nucleotides
RPSI07_RS22490: RPSI07_RS22490 - polyphenol oxidase, at 3,201,272 to 3,202,762
_RS22490
RPSI07_RS22495: RPSI07_RS22495 - hypothetical protein, at 3,202,809 to 3,203,282
_RS22495
RPSI07_RS22500: RPSI07_RS22500 - lysine transporter LysE, at 3,203,346 to 3,203,987
_RS22500
RPSI07_RS22505: RPSI07_RS22505 - hypothetical protein, at 3,204,069 to 3,204,638
_RS22505
Position (kb) 3202
3203
3204 Strain fitness (log2 ratio) -1
0
1 at 3202.036 kb on + strand, within RPSI07_RS22490 at 3202.124 kb on + strand, within RPSI07_RS22490 at 3202.124 kb on + strand, within RPSI07_RS22490 at 3202.124 kb on + strand, within RPSI07_RS22490 at 3202.124 kb on + strand, within RPSI07_RS22490 at 3202.124 kb on + strand, within RPSI07_RS22490 at 3202.125 kb on - strand, within RPSI07_RS22490 at 3202.125 kb on - strand, within RPSI07_RS22490 at 3202.125 kb on - strand, within RPSI07_RS22490 at 3202.125 kb on - strand, within RPSI07_RS22490 at 3202.145 kb on + strand, within RPSI07_RS22490 at 3202.145 kb on + strand, within RPSI07_RS22490 at 3202.146 kb on - strand, within RPSI07_RS22490 at 3202.148 kb on + strand, within RPSI07_RS22490 at 3202.148 kb on + strand, within RPSI07_RS22490 at 3202.148 kb on + strand, within RPSI07_RS22490 at 3202.149 kb on - strand, within RPSI07_RS22490 at 3202.432 kb on + strand, within RPSI07_RS22490 at 3202.433 kb on - strand, within RPSI07_RS22490 at 3202.433 kb on - strand, within RPSI07_RS22490 at 3202.433 kb on - strand, within RPSI07_RS22490 at 3202.550 kb on + strand, within RPSI07_RS22490 at 3202.551 kb on - strand, within RPSI07_RS22490 at 3202.551 kb on - strand, within RPSI07_RS22490 at 3202.705 kb on - strand at 3202.808 kb on + strand at 3202.808 kb on + strand at 3202.809 kb on - strand at 3202.809 kb on - strand at 3202.809 kb on - strand at 3203.049 kb on + strand, within RPSI07_RS22495 at 3203.049 kb on + strand, within RPSI07_RS22495 at 3203.106 kb on + strand, within RPSI07_RS22495 at 3203.106 kb on + strand, within RPSI07_RS22495 at 3203.106 kb on + strand, within RPSI07_RS22495 at 3203.107 kb on - strand, within RPSI07_RS22495 at 3203.107 kb on - strand, within RPSI07_RS22495 at 3203.302 kb on + strand at 3203.347 kb on + strand at 3203.734 kb on + strand, within RPSI07_RS22500 at 3203.735 kb on - strand, within RPSI07_RS22500 at 3204.045 kb on - strand at 3204.045 kb on - strand at 3204.074 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 3,202,036 + RPSI07_RS22490 0.51 +0.2 3,202,124 + RPSI07_RS22490 0.57 +0.0 3,202,124 + RPSI07_RS22490 0.57 +1.0 3,202,124 + RPSI07_RS22490 0.57 +1.0 3,202,124 + RPSI07_RS22490 0.57 +1.0 3,202,124 + RPSI07_RS22490 0.57 -0.4 3,202,125 - RPSI07_RS22490 0.57 +1.0 3,202,125 - RPSI07_RS22490 0.57 +1.5 3,202,125 - RPSI07_RS22490 0.57 -0.3 3,202,125 - RPSI07_RS22490 0.57 +0.1 3,202,145 + RPSI07_RS22490 0.59 -0.4 3,202,145 + RPSI07_RS22490 0.59 -0.2 3,202,146 - RPSI07_RS22490 0.59 +1.0 3,202,148 + RPSI07_RS22490 0.59 +0.4 3,202,148 + RPSI07_RS22490 0.59 +0.0 3,202,148 + RPSI07_RS22490 0.59 -0.5 3,202,149 - RPSI07_RS22490 0.59 -0.3 3,202,432 + RPSI07_RS22490 0.78 -1.0 3,202,433 - RPSI07_RS22490 0.78 -0.8 3,202,433 - RPSI07_RS22490 0.78 +1.0 3,202,433 - RPSI07_RS22490 0.78 -0.2 3,202,550 + RPSI07_RS22490 0.86 -0.5 3,202,551 - RPSI07_RS22490 0.86 +1.0 3,202,551 - RPSI07_RS22490 0.86 +0.5 3,202,705 - -0.1 3,202,808 + +0.0 3,202,808 + +1.0 3,202,809 - +0.2 3,202,809 - +1.0 3,202,809 - -1.1 3,203,049 + RPSI07_RS22495 0.51 -0.5 3,203,049 + RPSI07_RS22495 0.51 +0.2 3,203,106 + RPSI07_RS22495 0.63 +0.1 3,203,106 + RPSI07_RS22495 0.63 +1.0 3,203,106 + RPSI07_RS22495 0.63 -0.8 3,203,107 - RPSI07_RS22495 0.63 +1.0 3,203,107 - RPSI07_RS22495 0.63 +0.7 3,203,302 + +0.7 3,203,347 + +0.0 3,203,734 + RPSI07_RS22500 0.60 +0.6 3,203,735 - RPSI07_RS22500 0.61 +1.0 3,204,045 - +1.0 3,204,045 - +0.4 3,204,074 - +1.5
Or see this region's nucleotide sequence