Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS23740 and RPSI07_RS23745 are separated by 214 nucleotides RPSI07_RS23745 and RPSI07_RS23750 are separated by 197 nucleotides RPSI07_RS23750 and RPSI07_RS23755 are separated by 47 nucleotides
RPSI07_RS23740: RPSI07_RS23740 - ATP-dependent protease ATPase subunit HslU, at 3,470,826 to 3,472,160
_RS23740
RPSI07_RS23745: RPSI07_RS23745 - hypothetical protein, at 3,472,375 to 3,473,667
_RS23745
RPSI07_RS23750: RPSI07_RS23750 - DNA-binding response regulator, at 3,473,865 to 3,474,443
_RS23750
RPSI07_RS23755: RPSI07_RS23755 - sensor histidine kinase, at 3,474,491 to 3,475,837
_RS23755
Position (kb)
3472
3473
3474 Strain fitness (log2 ratio)
-2
-1
0
1 at 3471.708 kb on + strand, within RPSI07_RS23740 at 3471.708 kb on + strand, within RPSI07_RS23740 at 3471.708 kb on + strand, within RPSI07_RS23740 at 3471.709 kb on - strand, within RPSI07_RS23740 at 3472.014 kb on + strand, within RPSI07_RS23740 at 3472.014 kb on + strand, within RPSI07_RS23740 at 3472.014 kb on + strand, within RPSI07_RS23740 at 3472.015 kb on - strand, within RPSI07_RS23740 at 3472.015 kb on - strand, within RPSI07_RS23740 at 3472.015 kb on - strand, within RPSI07_RS23740 at 3472.015 kb on - strand, within RPSI07_RS23740 at 3472.098 kb on + strand at 3472.099 kb on - strand at 3472.099 kb on - strand at 3472.099 kb on - strand at 3472.149 kb on + strand at 3472.159 kb on - strand at 3472.226 kb on + strand at 3472.250 kb on - strand at 3472.520 kb on + strand, within RPSI07_RS23745 at 3472.520 kb on + strand, within RPSI07_RS23745 at 3472.520 kb on + strand, within RPSI07_RS23745 at 3472.520 kb on + strand, within RPSI07_RS23745 at 3472.521 kb on - strand, within RPSI07_RS23745 at 3472.521 kb on - strand, within RPSI07_RS23745 at 3472.521 kb on - strand, within RPSI07_RS23745 at 3472.617 kb on - strand, within RPSI07_RS23745 at 3472.617 kb on - strand, within RPSI07_RS23745 at 3472.617 kb on - strand, within RPSI07_RS23745 at 3472.691 kb on + strand, within RPSI07_RS23745 at 3472.692 kb on - strand, within RPSI07_RS23745 at 3472.692 kb on - strand, within RPSI07_RS23745 at 3472.759 kb on - strand, within RPSI07_RS23745 at 3472.823 kb on + strand, within RPSI07_RS23745 at 3472.841 kb on + strand, within RPSI07_RS23745 at 3472.841 kb on + strand, within RPSI07_RS23745 at 3472.842 kb on - strand, within RPSI07_RS23745 at 3472.842 kb on - strand, within RPSI07_RS23745 at 3472.953 kb on + strand, within RPSI07_RS23745 at 3472.955 kb on + strand, within RPSI07_RS23745 at 3472.955 kb on + strand, within RPSI07_RS23745 at 3472.955 kb on + strand, within RPSI07_RS23745 at 3472.955 kb on + strand, within RPSI07_RS23745 at 3472.956 kb on - strand, within RPSI07_RS23745 at 3472.956 kb on - strand, within RPSI07_RS23745 at 3472.956 kb on - strand, within RPSI07_RS23745 at 3473.054 kb on + strand, within RPSI07_RS23745 at 3473.054 kb on + strand, within RPSI07_RS23745 at 3473.055 kb on - strand, within RPSI07_RS23745 at 3473.108 kb on + strand, within RPSI07_RS23745 at 3473.108 kb on + strand, within RPSI07_RS23745 at 3473.108 kb on + strand, within RPSI07_RS23745 at 3473.108 kb on + strand at 3473.109 kb on - strand, within RPSI07_RS23745 at 3473.254 kb on + strand, within RPSI07_RS23745 at 3473.255 kb on - strand, within RPSI07_RS23745 at 3473.255 kb on - strand, within RPSI07_RS23745 at 3473.255 kb on - strand, within RPSI07_RS23745 at 3473.462 kb on + strand, within RPSI07_RS23745 at 3473.487 kb on + strand, within RPSI07_RS23745 at 3473.487 kb on + strand, within RPSI07_RS23745 at 3473.488 kb on - strand, within RPSI07_RS23745 at 3473.488 kb on - strand, within RPSI07_RS23745 at 3473.488 kb on - strand, within RPSI07_RS23745 at 3473.605 kb on + strand at 3473.659 kb on - strand at 3473.659 kb on - strand at 3473.659 kb on - strand at 3473.659 kb on - strand at 3473.671 kb on + strand at 3473.671 kb on + strand at 3473.671 kb on + strand at 3473.672 kb on - strand at 3473.672 kb on - strand at 3473.672 kb on - strand at 3473.672 kb on - strand at 3473.672 kb on - strand at 3473.672 kb on - strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.678 kb on - strand at 3473.678 kb on - strand at 3473.678 kb on - strand at 3473.683 kb on + strand at 3473.683 kb on + strand at 3473.683 kb on + strand at 3473.768 kb on + strand at 3473.768 kb on + strand at 3473.768 kb on + strand at 3473.768 kb on + strand at 3473.768 kb on + strand at 3473.769 kb on - strand at 3473.769 kb on - strand at 3473.769 kb on - strand at 3473.769 kb on - strand at 3473.769 kb on - strand at 3473.866 kb on + strand at 3473.866 kb on + strand at 3473.866 kb on + strand at 3473.867 kb on - strand at 3473.867 kb on - strand at 3473.867 kb on - strand at 3474.106 kb on + strand, within RPSI07_RS23750 at 3474.106 kb on + strand, within RPSI07_RS23750 at 3474.106 kb on + strand, within RPSI07_RS23750 at 3474.106 kb on + strand, within RPSI07_RS23750 at 3474.107 kb on - strand, within RPSI07_RS23750 at 3474.107 kb on - strand, within RPSI07_RS23750 at 3474.293 kb on - strand, within RPSI07_RS23750 at 3474.293 kb on - strand, within RPSI07_RS23750 at 3474.496 kb on + strand at 3474.496 kb on + strand at 3474.497 kb on - strand at 3474.497 kb on - strand at 3474.541 kb on + strand at 3474.541 kb on + strand at 3474.542 kb on - strand at 3474.542 kb on - strand at 3474.542 kb on - strand at 3474.611 kb on - strand at 3474.645 kb on + strand, within RPSI07_RS23755 at 3474.645 kb on + strand, within RPSI07_RS23755 at 3474.646 kb on - strand, within RPSI07_RS23755 at 3474.646 kb on - strand, within RPSI07_RS23755
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 3,471,708 + RPSI07_RS23740 0.66 +1.5 3,471,708 + RPSI07_RS23740 0.66 -0.4 3,471,708 + RPSI07_RS23740 0.66 -0.3 3,471,709 - RPSI07_RS23740 0.66 +1.1 3,472,014 + RPSI07_RS23740 0.89 +0.4 3,472,014 + RPSI07_RS23740 0.89 -0.4 3,472,014 + RPSI07_RS23740 0.89 -0.3 3,472,015 - RPSI07_RS23740 0.89 -0.5 3,472,015 - RPSI07_RS23740 0.89 +0.1 3,472,015 - RPSI07_RS23740 0.89 +1.1 3,472,015 - RPSI07_RS23740 0.89 -0.5 3,472,098 + -0.9 3,472,099 - -0.9 3,472,099 - +1.1 3,472,099 - +1.1 3,472,149 + -0.1 3,472,159 - -0.3 3,472,226 + +0.1 3,472,250 - -0.1 3,472,520 + RPSI07_RS23745 0.11 +1.1 3,472,520 + RPSI07_RS23745 0.11 -0.6 3,472,520 + RPSI07_RS23745 0.11 -0.1 3,472,520 + RPSI07_RS23745 0.11 +1.1 3,472,521 - RPSI07_RS23745 0.11 -0.9 3,472,521 - RPSI07_RS23745 0.11 +0.5 3,472,521 - RPSI07_RS23745 0.11 +0.1 3,472,617 - RPSI07_RS23745 0.19 +0.8 3,472,617 - RPSI07_RS23745 0.19 +0.1 3,472,617 - RPSI07_RS23745 0.19 +0.1 3,472,691 + RPSI07_RS23745 0.24 +0.1 3,472,692 - RPSI07_RS23745 0.25 -1.6 3,472,692 - RPSI07_RS23745 0.25 -0.2 3,472,759 - RPSI07_RS23745 0.30 -0.1 3,472,823 + RPSI07_RS23745 0.35 +0.1 3,472,841 + RPSI07_RS23745 0.36 +0.2 3,472,841 + RPSI07_RS23745 0.36 -0.9 3,472,842 - RPSI07_RS23745 0.36 -0.4 3,472,842 - RPSI07_RS23745 0.36 -0.2 3,472,953 + RPSI07_RS23745 0.45 +1.1 3,472,955 + RPSI07_RS23745 0.45 -0.9 3,472,955 + RPSI07_RS23745 0.45 -0.7 3,472,955 + RPSI07_RS23745 0.45 +1.1 3,472,955 + RPSI07_RS23745 0.45 -0.1 3,472,956 - RPSI07_RS23745 0.45 -0.3 3,472,956 - RPSI07_RS23745 0.45 +0.3 3,472,956 - RPSI07_RS23745 0.45 +1.1 3,473,054 + RPSI07_RS23745 0.53 -0.8 3,473,054 + RPSI07_RS23745 0.53 -0.5 3,473,055 - RPSI07_RS23745 0.53 +0.2 3,473,108 + RPSI07_RS23745 0.57 +1.1 3,473,108 + RPSI07_RS23745 0.57 +1.4 3,473,108 + RPSI07_RS23745 0.57 +0.1 3,473,108 + -0.3 3,473,109 - RPSI07_RS23745 0.57 -0.7 3,473,254 + RPSI07_RS23745 0.68 -0.8 3,473,255 - RPSI07_RS23745 0.68 -0.9 3,473,255 - RPSI07_RS23745 0.68 -0.1 3,473,255 - RPSI07_RS23745 0.68 -0.3 3,473,462 + RPSI07_RS23745 0.84 +0.2 3,473,487 + RPSI07_RS23745 0.86 -0.3 3,473,487 + RPSI07_RS23745 0.86 -0.5 3,473,488 - RPSI07_RS23745 0.86 +0.8 3,473,488 - RPSI07_RS23745 0.86 +0.1 3,473,488 - RPSI07_RS23745 0.86 +0.1 3,473,605 + +0.9 3,473,659 - +0.8 3,473,659 - -1.0 3,473,659 - -0.9 3,473,659 - -0.3 3,473,671 + -0.7 3,473,671 + -0.5 3,473,671 + -0.9 3,473,672 - +0.8 3,473,672 - +0.3 3,473,672 - -0.2 3,473,672 - +1.4 3,473,672 - +0.2 3,473,672 - -0.1 3,473,677 + +0.1 3,473,677 + -0.0 3,473,677 + +0.5 3,473,677 + +1.3 3,473,677 + +0.3 3,473,677 + -0.9 3,473,677 + -0.7 3,473,677 + +0.4 3,473,677 + +0.2 3,473,677 + +0.8 3,473,678 - +0.5 3,473,678 - -0.5 3,473,678 - -1.1 3,473,683 + +0.1 3,473,683 + +0.3 3,473,683 + +1.1 3,473,768 + -0.5 3,473,768 + +0.1 3,473,768 + +0.4 3,473,768 + -0.9 3,473,768 + +0.2 3,473,769 - -0.6 3,473,769 - -1.3 3,473,769 - +1.1 3,473,769 - +0.9 3,473,769 - -0.5 3,473,866 + +1.1 3,473,866 + +1.1 3,473,866 + -0.3 3,473,867 - -0.2 3,473,867 - +0.5 3,473,867 - +0.1 3,474,106 + RPSI07_RS23750 0.42 -0.7 3,474,106 + RPSI07_RS23750 0.42 -0.6 3,474,106 + RPSI07_RS23750 0.42 -2.6 3,474,106 + RPSI07_RS23750 0.42 -0.5 3,474,107 - RPSI07_RS23750 0.42 -1.3 3,474,107 - RPSI07_RS23750 0.42 -1.3 3,474,293 - RPSI07_RS23750 0.74 -0.5 3,474,293 - RPSI07_RS23750 0.74 -1.5 3,474,496 + -1.2 3,474,496 + -0.3 3,474,497 - +1.1 3,474,497 - -0.5 3,474,541 + -1.1 3,474,541 + +0.3 3,474,542 - +1.1 3,474,542 - +1.1 3,474,542 - +0.0 3,474,611 - -0.1 3,474,645 + RPSI07_RS23755 0.11 -0.7 3,474,645 + RPSI07_RS23755 0.11 +0.1 3,474,646 - RPSI07_RS23755 0.12 +0.1 3,474,646 - RPSI07_RS23755 0.12 +0.1
Or see this region's nucleotide sequence