Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS23575 and RPSI07_RS23580 are separated by 94 nucleotides RPSI07_RS23580 and RPSI07_RS23585 are separated by 119 nucleotides RPSI07_RS23585 and RPSI07_RS23590 are separated by 34 nucleotides
RPSI07_RS23575: RPSI07_RS23575 - 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB, at 3,430,994 to 3,432,319
_RS23575
RPSI07_RS23580: RPSI07_RS23580 - zinc metalloprotease HtpX, at 3,432,414 to 3,433,274
_RS23580
RPSI07_RS23585: RPSI07_RS23585 - LysE family translocator, at 3,433,394 to 3,434,041
_RS23585
RPSI07_RS23590: RPSI07_RS23590 - methionyl-tRNA formyltransferase, at 3,434,076 to 3,435,059
_RS23590
Position (kb)
3432
3433
3434 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3431.745 kb on + strand, within RPSI07_RS23575 at 3431.838 kb on + strand, within RPSI07_RS23575 at 3431.874 kb on + strand, within RPSI07_RS23575 at 3431.875 kb on - strand, within RPSI07_RS23575 at 3432.049 kb on + strand, within RPSI07_RS23575 at 3432.165 kb on + strand, within RPSI07_RS23575 at 3432.396 kb on + strand at 3432.397 kb on - strand at 3432.397 kb on - strand at 3432.397 kb on - strand at 3432.397 kb on - strand at 3432.397 kb on - strand at 3432.397 kb on - strand at 3432.398 kb on + strand at 3432.398 kb on + strand at 3432.399 kb on - strand at 3432.399 kb on - strand at 3432.425 kb on - strand at 3432.590 kb on - strand, within RPSI07_RS23580 at 3433.010 kb on - strand, within RPSI07_RS23580 at 3433.063 kb on + strand, within RPSI07_RS23580 at 3433.105 kb on + strand, within RPSI07_RS23580 at 3433.105 kb on + strand, within RPSI07_RS23580 at 3433.105 kb on + strand, within RPSI07_RS23580 at 3433.106 kb on - strand, within RPSI07_RS23580 at 3433.314 kb on + strand at 3433.314 kb on + strand at 3433.314 kb on + strand at 3433.314 kb on + strand at 3433.314 kb on + strand at 3433.315 kb on - strand at 3433.315 kb on - strand at 3433.315 kb on - strand at 3433.315 kb on - strand at 3433.324 kb on + strand at 3433.324 kb on + strand at 3433.324 kb on + strand at 3433.325 kb on - strand at 3433.499 kb on + strand, within RPSI07_RS23585 at 3433.779 kb on + strand, within RPSI07_RS23585 at 3433.780 kb on - strand, within RPSI07_RS23585 at 3433.780 kb on - strand, within RPSI07_RS23585 at 3433.780 kb on - strand, within RPSI07_RS23585 at 3433.780 kb on - strand, within RPSI07_RS23585 at 3433.780 kb on - strand, within RPSI07_RS23585 at 3433.789 kb on - strand, within RPSI07_RS23585 at 3433.954 kb on + strand, within RPSI07_RS23585 at 3433.954 kb on + strand, within RPSI07_RS23585 at 3433.954 kb on + strand, within RPSI07_RS23585 at 3433.955 kb on - strand, within RPSI07_RS23585 at 3433.955 kb on - strand, within RPSI07_RS23585 at 3433.956 kb on + strand, within RPSI07_RS23585 at 3433.956 kb on + strand, within RPSI07_RS23585 at 3433.956 kb on + strand, within RPSI07_RS23585 at 3433.957 kb on - strand, within RPSI07_RS23585 at 3434.035 kb on + strand at 3434.036 kb on - strand at 3434.036 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 3,431,745 + RPSI07_RS23575 0.57 +2.1 3,431,838 + RPSI07_RS23575 0.64 -0.5 3,431,874 + RPSI07_RS23575 0.66 +0.2 3,431,875 - RPSI07_RS23575 0.66 -0.2 3,432,049 + RPSI07_RS23575 0.80 +1.1 3,432,165 + RPSI07_RS23575 0.88 -0.6 3,432,396 + -0.5 3,432,397 - -0.4 3,432,397 - -0.3 3,432,397 - -0.4 3,432,397 - -0.6 3,432,397 - -0.2 3,432,397 - -0.2 3,432,398 + -1.9 3,432,398 + -0.1 3,432,399 - -0.6 3,432,399 - -0.4 3,432,425 - -0.4 3,432,590 - RPSI07_RS23580 0.20 +1.1 3,433,010 - RPSI07_RS23580 0.69 -0.4 3,433,063 + RPSI07_RS23580 0.75 +0.1 3,433,105 + RPSI07_RS23580 0.80 -0.9 3,433,105 + RPSI07_RS23580 0.80 -0.5 3,433,105 + RPSI07_RS23580 0.80 +0.5 3,433,106 - RPSI07_RS23580 0.80 -0.1 3,433,314 + +1.6 3,433,314 + +1.1 3,433,314 + -0.5 3,433,314 + +0.1 3,433,314 + -0.1 3,433,315 - +0.2 3,433,315 - -0.8 3,433,315 - +0.1 3,433,315 - -0.7 3,433,324 + -0.1 3,433,324 + -0.3 3,433,324 + +1.1 3,433,325 - +2.6 3,433,499 + RPSI07_RS23585 0.16 -1.7 3,433,779 + RPSI07_RS23585 0.59 +0.3 3,433,780 - RPSI07_RS23585 0.60 +0.5 3,433,780 - RPSI07_RS23585 0.60 +0.4 3,433,780 - RPSI07_RS23585 0.60 +0.3 3,433,780 - RPSI07_RS23585 0.60 +0.5 3,433,780 - RPSI07_RS23585 0.60 +0.0 3,433,789 - RPSI07_RS23585 0.61 -1.2 3,433,954 + RPSI07_RS23585 0.86 -0.5 3,433,954 + RPSI07_RS23585 0.86 +1.1 3,433,954 + RPSI07_RS23585 0.86 +1.1 3,433,955 - RPSI07_RS23585 0.87 +0.5 3,433,955 - RPSI07_RS23585 0.87 +1.1 3,433,956 + RPSI07_RS23585 0.87 -0.9 3,433,956 + RPSI07_RS23585 0.87 +1.1 3,433,956 + RPSI07_RS23585 0.87 -0.3 3,433,957 - RPSI07_RS23585 0.87 -0.0 3,434,035 + +0.2 3,434,036 - +0.6 3,434,036 - +0.5
Or see this region's nucleotide sequence