Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS12650 and RPSI07_RS12655 overlap by 4 nucleotides RPSI07_RS12655 and RPSI07_RS12660 are separated by 6 nucleotides RPSI07_RS12660 and RPSI07_RS12665 are separated by 162 nucleotides RPSI07_RS12665 and RPSI07_RS12670 are separated by 13 nucleotides RPSI07_RS12670 and RPSI07_RS12675 are separated by 173 nucleotides
RPSI07_RS12650: RPSI07_RS12650 - quinolinate synthase NadA, at 1,029,662 to 1,030,810
_RS12650
RPSI07_RS12655: RPSI07_RS12655 - nicotinate-nucleotide diphosphorylase (carboxylating), at 1,030,807 to 1,031,694
_RS12655
RPSI07_RS12660: RPSI07_RS12660 - L-aspartate oxidase, at 1,031,701 to 1,033,302
_RS12660
RPSI07_RS12665: RPSI07_RS12665 - 50S ribosomal protein L33, at 1,033,465 to 1,033,635
_RS12665
RPSI07_RS12670: RPSI07_RS12670 - 50S ribosomal protein L28, at 1,033,649 to 1,033,882
_RS12670
RPSI07_RS12675: RPSI07_RS12675 - JAB domain-containing protein, at 1,034,056 to 1,034,730
_RS12675
Position (kb)
1031
1032
1033
1034 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1030.708 kb on + strand at 1030.708 kb on + strand at 1030.709 kb on - strand at 1031.290 kb on + strand, within RPSI07_RS12655 at 1031.290 kb on + strand, within RPSI07_RS12655 at 1031.290 kb on + strand, within RPSI07_RS12655 at 1031.291 kb on - strand, within RPSI07_RS12655 at 1031.291 kb on - strand, within RPSI07_RS12655 at 1031.291 kb on - strand, within RPSI07_RS12655 at 1031.336 kb on - strand, within RPSI07_RS12655 at 1031.671 kb on + strand at 1031.672 kb on - strand at 1031.672 kb on - strand at 1031.692 kb on + strand at 1031.692 kb on + strand at 1031.693 kb on - strand at 1031.775 kb on - strand at 1031.897 kb on + strand, within RPSI07_RS12660 at 1031.897 kb on + strand, within RPSI07_RS12660 at 1031.898 kb on - strand, within RPSI07_RS12660 at 1031.964 kb on + strand, within RPSI07_RS12660 at 1031.981 kb on + strand, within RPSI07_RS12660 at 1031.981 kb on + strand, within RPSI07_RS12660 at 1031.982 kb on - strand, within RPSI07_RS12660 at 1031.982 kb on - strand, within RPSI07_RS12660 at 1031.982 kb on - strand, within RPSI07_RS12660 at 1032.197 kb on + strand, within RPSI07_RS12660 at 1032.197 kb on + strand, within RPSI07_RS12660 at 1032.197 kb on + strand, within RPSI07_RS12660 at 1032.218 kb on + strand, within RPSI07_RS12660 at 1032.275 kb on + strand, within RPSI07_RS12660 at 1032.275 kb on + strand, within RPSI07_RS12660 at 1032.275 kb on + strand, within RPSI07_RS12660 at 1032.275 kb on + strand, within RPSI07_RS12660 at 1032.275 kb on + strand, within RPSI07_RS12660 at 1032.276 kb on - strand, within RPSI07_RS12660 at 1032.276 kb on - strand, within RPSI07_RS12660 at 1032.401 kb on + strand, within RPSI07_RS12660 at 1032.401 kb on + strand, within RPSI07_RS12660 at 1032.401 kb on + strand, within RPSI07_RS12660 at 1032.402 kb on - strand, within RPSI07_RS12660 at 1032.402 kb on - strand, within RPSI07_RS12660 at 1032.584 kb on + strand, within RPSI07_RS12660 at 1032.692 kb on + strand, within RPSI07_RS12660 at 1032.692 kb on + strand, within RPSI07_RS12660 at 1032.693 kb on - strand, within RPSI07_RS12660 at 1032.693 kb on - strand, within RPSI07_RS12660 at 1032.698 kb on + strand, within RPSI07_RS12660 at 1032.699 kb on - strand, within RPSI07_RS12660 at 1032.782 kb on + strand, within RPSI07_RS12660 at 1032.783 kb on - strand, within RPSI07_RS12660 at 1032.783 kb on - strand, within RPSI07_RS12660 at 1032.883 kb on + strand, within RPSI07_RS12660 at 1032.884 kb on - strand, within RPSI07_RS12660 at 1033.376 kb on + strand at 1033.376 kb on + strand at 1033.376 kb on + strand at 1033.376 kb on + strand at 1033.377 kb on - strand at 1033.467 kb on - strand at 1033.487 kb on + strand, within RPSI07_RS12665 at 1033.647 kb on - strand at 1033.650 kb on + strand at 1034.058 kb on - strand at 1034.095 kb on - strand at 1034.095 kb on - strand at 1034.243 kb on + strand, within RPSI07_RS12675 at 1034.243 kb on + strand, within RPSI07_RS12675 at 1034.243 kb on + strand, within RPSI07_RS12675 at 1034.244 kb on - strand, within RPSI07_RS12675
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 1,030,708 + +0.3 1,030,708 + +0.4 1,030,709 - -0.1 1,031,290 + RPSI07_RS12655 0.54 +1.0 1,031,290 + RPSI07_RS12655 0.54 -0.6 1,031,290 + RPSI07_RS12655 0.54 -0.8 1,031,291 - RPSI07_RS12655 0.55 -1.1 1,031,291 - RPSI07_RS12655 0.55 -0.2 1,031,291 - RPSI07_RS12655 0.55 -1.2 1,031,336 - RPSI07_RS12655 0.60 -0.4 1,031,671 + -1.3 1,031,672 - -0.5 1,031,672 - +0.4 1,031,692 + -0.5 1,031,692 + +0.7 1,031,693 - +0.0 1,031,775 - +1.0 1,031,897 + RPSI07_RS12660 0.12 +1.0 1,031,897 + RPSI07_RS12660 0.12 +0.1 1,031,898 - RPSI07_RS12660 0.12 +0.0 1,031,964 + RPSI07_RS12660 0.16 +2.0 1,031,981 + RPSI07_RS12660 0.17 -0.6 1,031,981 + RPSI07_RS12660 0.17 +1.3 1,031,982 - RPSI07_RS12660 0.18 -0.2 1,031,982 - RPSI07_RS12660 0.18 -0.4 1,031,982 - RPSI07_RS12660 0.18 -1.1 1,032,197 + RPSI07_RS12660 0.31 +1.0 1,032,197 + RPSI07_RS12660 0.31 +1.0 1,032,197 + RPSI07_RS12660 0.31 +1.0 1,032,218 + RPSI07_RS12660 0.32 +0.7 1,032,275 + RPSI07_RS12660 0.36 +0.0 1,032,275 + RPSI07_RS12660 0.36 -0.6 1,032,275 + RPSI07_RS12660 0.36 +0.1 1,032,275 + RPSI07_RS12660 0.36 -0.9 1,032,275 + RPSI07_RS12660 0.36 +0.2 1,032,276 - RPSI07_RS12660 0.36 -0.2 1,032,276 - RPSI07_RS12660 0.36 -0.3 1,032,401 + RPSI07_RS12660 0.44 +0.4 1,032,401 + RPSI07_RS12660 0.44 +0.3 1,032,401 + RPSI07_RS12660 0.44 -1.3 1,032,402 - RPSI07_RS12660 0.44 +0.5 1,032,402 - RPSI07_RS12660 0.44 +1.0 1,032,584 + RPSI07_RS12660 0.55 -0.5 1,032,692 + RPSI07_RS12660 0.62 -1.0 1,032,692 + RPSI07_RS12660 0.62 -0.5 1,032,693 - RPSI07_RS12660 0.62 +0.6 1,032,693 - RPSI07_RS12660 0.62 +0.1 1,032,698 + RPSI07_RS12660 0.62 -0.4 1,032,699 - RPSI07_RS12660 0.62 -1.4 1,032,782 + RPSI07_RS12660 0.67 +0.3 1,032,783 - RPSI07_RS12660 0.68 -0.5 1,032,783 - RPSI07_RS12660 0.68 +1.0 1,032,883 + RPSI07_RS12660 0.74 +0.2 1,032,884 - RPSI07_RS12660 0.74 -0.3 1,033,376 + -1.8 1,033,376 + -0.6 1,033,376 + -2.3 1,033,376 + +1.0 1,033,377 - -0.2 1,033,467 - +1.6 1,033,487 + RPSI07_RS12665 0.13 +1.0 1,033,647 - +1.0 1,033,650 + -0.6 1,034,058 - -0.2 1,034,095 - -0.0 1,034,095 - +0.5 1,034,243 + RPSI07_RS12675 0.28 -0.5 1,034,243 + RPSI07_RS12675 0.28 -0.5 1,034,243 + RPSI07_RS12675 0.28 -0.7 1,034,244 - RPSI07_RS12675 0.28 +0.2
Or see this region's nucleotide sequence