Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS12070 and RPSI07_RS12075 are separated by 60 nucleotides RPSI07_RS12075 and RPSI07_RS12080 are separated by 53 nucleotides
RPSI07_RS12070: RPSI07_RS12070 - DNA mismatch repair endonuclease MutL, at 909,747 to 911,660
_RS12070
RPSI07_RS12075: RPSI07_RS12075 - MCE family protein, at 911,721 to 913,397
_RS12075
RPSI07_RS12080: RPSI07_RS12080 - DUF3313 domain-containing protein, at 913,451 to 914,113
_RS12080
Position (kb) 911
912
913
914 Strain fitness (log2 ratio) -1
0
1
2 at 910.801 kb on + strand, within RPSI07_RS12070 at 910.802 kb on - strand, within RPSI07_RS12070 at 910.802 kb on - strand, within RPSI07_RS12070 at 910.838 kb on - strand, within RPSI07_RS12070 at 910.895 kb on - strand, within RPSI07_RS12070 at 911.198 kb on - strand, within RPSI07_RS12070 at 911.198 kb on - strand, within RPSI07_RS12070 at 911.520 kb on - strand at 911.670 kb on - strand at 911.671 kb on + strand at 911.671 kb on + strand at 911.672 kb on - strand at 911.672 kb on - strand at 911.672 kb on - strand at 912.124 kb on + strand, within RPSI07_RS12075 at 912.169 kb on + strand, within RPSI07_RS12075 at 912.169 kb on + strand, within RPSI07_RS12075 at 912.170 kb on - strand, within RPSI07_RS12075 at 912.677 kb on + strand, within RPSI07_RS12075 at 912.700 kb on + strand, within RPSI07_RS12075 at 912.793 kb on + strand, within RPSI07_RS12075 at 912.943 kb on + strand, within RPSI07_RS12075 at 912.943 kb on + strand, within RPSI07_RS12075 at 912.943 kb on + strand, within RPSI07_RS12075 at 912.943 kb on + strand, within RPSI07_RS12075 at 912.943 kb on + strand, within RPSI07_RS12075 at 912.943 kb on + strand, within RPSI07_RS12075 at 912.943 kb on + strand, within RPSI07_RS12075 at 912.944 kb on - strand, within RPSI07_RS12075 at 912.944 kb on - strand, within RPSI07_RS12075 at 912.944 kb on - strand, within RPSI07_RS12075 at 912.944 kb on - strand, within RPSI07_RS12075 at 912.944 kb on - strand, within RPSI07_RS12075 at 912.944 kb on - strand, within RPSI07_RS12075 at 913.132 kb on + strand, within RPSI07_RS12075 at 913.133 kb on - strand, within RPSI07_RS12075 at 913.133 kb on - strand, within RPSI07_RS12075 at 913.133 kb on - strand, within RPSI07_RS12075 at 913.452 kb on + strand at 913.453 kb on - strand at 913.453 kb on - strand at 913.566 kb on + strand, within RPSI07_RS12080 at 913.566 kb on + strand, within RPSI07_RS12080 at 913.567 kb on - strand, within RPSI07_RS12080 at 913.600 kb on - strand, within RPSI07_RS12080 at 913.614 kb on + strand, within RPSI07_RS12080 at 913.615 kb on - strand, within RPSI07_RS12080 at 913.671 kb on + strand, within RPSI07_RS12080 at 913.671 kb on + strand, within RPSI07_RS12080 at 913.671 kb on + strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.830 kb on + strand, within RPSI07_RS12080 at 913.830 kb on + strand, within RPSI07_RS12080 at 913.830 kb on + strand, within RPSI07_RS12080 at 913.830 kb on + strand, within RPSI07_RS12080 at 913.831 kb on - strand at 913.893 kb on + strand, within RPSI07_RS12080 at 913.926 kb on + strand, within RPSI07_RS12080 at 913.953 kb on + strand, within RPSI07_RS12080 at 913.953 kb on + strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.959 kb on + strand, within RPSI07_RS12080 at 914.001 kb on + strand, within RPSI07_RS12080 at 914.001 kb on + strand, within RPSI07_RS12080 at 914.001 kb on + strand, within RPSI07_RS12080 at 914.001 kb on + strand, within RPSI07_RS12080 at 914.002 kb on - strand, within RPSI07_RS12080 at 914.002 kb on - strand, within RPSI07_RS12080 at 914.002 kb on - strand, within RPSI07_RS12080 at 914.196 kb on + strand at 914.196 kb on + strand at 914.196 kb on + strand at 914.196 kb on + strand at 914.197 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 910,801 + RPSI07_RS12070 0.55 +0.5 910,802 - RPSI07_RS12070 0.55 -0.5 910,802 - RPSI07_RS12070 0.55 -0.0 910,838 - RPSI07_RS12070 0.57 +0.7 910,895 - RPSI07_RS12070 0.60 +0.3 911,198 - RPSI07_RS12070 0.76 -0.5 911,198 - RPSI07_RS12070 0.76 +2.0 911,520 - -1.3 911,670 - +0.5 911,671 + -0.2 911,671 + -0.1 911,672 - -0.1 911,672 - -0.2 911,672 - -0.2 912,124 + RPSI07_RS12075 0.24 +0.4 912,169 + RPSI07_RS12075 0.27 +0.1 912,169 + RPSI07_RS12075 0.27 -0.1 912,170 - RPSI07_RS12075 0.27 -0.5 912,677 + RPSI07_RS12075 0.57 +0.0 912,700 + RPSI07_RS12075 0.58 +1.4 912,793 + RPSI07_RS12075 0.64 -1.0 912,943 + RPSI07_RS12075 0.73 -1.0 912,943 + RPSI07_RS12075 0.73 +0.2 912,943 + RPSI07_RS12075 0.73 -0.4 912,943 + RPSI07_RS12075 0.73 +0.0 912,943 + RPSI07_RS12075 0.73 +0.6 912,943 + RPSI07_RS12075 0.73 +0.0 912,943 + RPSI07_RS12075 0.73 -0.2 912,944 - RPSI07_RS12075 0.73 -0.8 912,944 - RPSI07_RS12075 0.73 -1.5 912,944 - RPSI07_RS12075 0.73 +0.1 912,944 - RPSI07_RS12075 0.73 -0.3 912,944 - RPSI07_RS12075 0.73 -0.6 912,944 - RPSI07_RS12075 0.73 +0.0 913,132 + RPSI07_RS12075 0.84 -0.2 913,133 - RPSI07_RS12075 0.84 -1.3 913,133 - RPSI07_RS12075 0.84 -0.4 913,133 - RPSI07_RS12075 0.84 +0.8 913,452 + -0.5 913,453 - -0.8 913,453 - -1.0 913,566 + RPSI07_RS12080 0.17 -0.1 913,566 + RPSI07_RS12080 0.17 +1.0 913,567 - RPSI07_RS12080 0.17 +0.5 913,600 - RPSI07_RS12080 0.22 +0.7 913,614 + RPSI07_RS12080 0.25 -0.4 913,615 - RPSI07_RS12080 0.25 -1.5 913,671 + RPSI07_RS12080 0.33 +0.5 913,671 + RPSI07_RS12080 0.33 +0.4 913,671 + RPSI07_RS12080 0.33 +1.0 913,672 - RPSI07_RS12080 0.33 +0.2 913,672 - RPSI07_RS12080 0.33 -0.7 913,672 - RPSI07_RS12080 0.33 -1.1 913,672 - RPSI07_RS12080 0.33 +0.5 913,672 - RPSI07_RS12080 0.33 +0.0 913,672 - RPSI07_RS12080 0.33 +1.0 913,672 - RPSI07_RS12080 0.33 +1.0 913,672 - RPSI07_RS12080 0.33 +0.7 913,672 - RPSI07_RS12080 0.33 +2.0 913,672 - RPSI07_RS12080 0.33 -0.4 913,830 + RPSI07_RS12080 0.57 +0.0 913,830 + RPSI07_RS12080 0.57 -0.5 913,830 + RPSI07_RS12080 0.57 -0.9 913,830 + RPSI07_RS12080 0.57 -0.5 913,831 - -0.5 913,893 + RPSI07_RS12080 0.67 +0.0 913,926 + RPSI07_RS12080 0.72 +0.0 913,953 + RPSI07_RS12080 0.76 +0.0 913,953 + RPSI07_RS12080 0.76 -0.5 913,954 - RPSI07_RS12080 0.76 +0.0 913,954 - RPSI07_RS12080 0.76 +0.7 913,954 - RPSI07_RS12080 0.76 -1.0 913,954 - RPSI07_RS12080 0.76 -0.3 913,954 - RPSI07_RS12080 0.76 +1.0 913,954 - RPSI07_RS12080 0.76 -1.1 913,954 - RPSI07_RS12080 0.76 +0.2 913,954 - RPSI07_RS12080 0.76 +0.4 913,959 + RPSI07_RS12080 0.77 +0.2 914,001 + RPSI07_RS12080 0.83 +1.0 914,001 + RPSI07_RS12080 0.83 -1.2 914,001 + RPSI07_RS12080 0.83 +0.2 914,001 + RPSI07_RS12080 0.83 -0.6 914,002 - RPSI07_RS12080 0.83 +1.0 914,002 - RPSI07_RS12080 0.83 -0.6 914,002 - RPSI07_RS12080 0.83 +0.4 914,196 + +0.8 914,196 + -0.5 914,196 + +0.9 914,196 + -0.3 914,197 - +0.0
Or see this region's nucleotide sequence