Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10635 and RPSI07_RS10640 are separated by 103 nucleotides RPSI07_RS10640 and RPSI07_RS10645 are separated by 190 nucleotides
RPSI07_RS10635: RPSI07_RS10635 - peptide chain release factor N(5)-glutamine methyltransferase, at 590,458 to 591,345
_RS10635
RPSI07_RS10640: RPSI07_RS10640 - hypothetical protein, at 591,449 to 591,781
_RS10640
RPSI07_RS10645: RPSI07_RS10645 - peptide chain release factor 1, at 591,972 to 593,054
_RS10645
Position (kb) 590
591
592 Strain fitness (log2 ratio) -1
0
1
2 at 589.498 kb on + strand at 589.557 kb on + strand at 589.623 kb on - strand at 589.630 kb on + strand at 589.630 kb on + strand at 589.631 kb on - strand at 589.631 kb on - strand at 589.631 kb on - strand at 589.732 kb on + strand at 589.774 kb on - strand at 589.777 kb on - strand at 589.824 kb on + strand at 589.824 kb on + strand at 589.825 kb on - strand at 589.825 kb on - strand at 589.898 kb on + strand at 589.898 kb on + strand at 589.898 kb on + strand at 589.899 kb on - strand at 589.899 kb on - strand at 589.899 kb on - strand at 589.919 kb on + strand at 589.957 kb on - strand at 589.962 kb on + strand at 589.963 kb on - strand at 589.963 kb on - strand at 589.971 kb on - strand at 590.019 kb on + strand at 590.206 kb on - strand at 590.256 kb on - strand at 590.256 kb on - strand at 590.281 kb on + strand at 590.399 kb on + strand at 590.442 kb on - strand at 591.281 kb on - strand at 591.383 kb on + strand at 591.768 kb on - strand at 591.793 kb on + strand at 591.799 kb on + strand at 591.800 kb on - strand at 591.889 kb on + strand at 591.889 kb on + strand at 591.889 kb on + strand at 591.890 kb on - strand at 591.890 kb on - strand at 591.890 kb on - strand at 591.891 kb on + strand at 591.948 kb on + strand at 591.949 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 589,498 + -0.3 589,557 + -0.2 589,623 - -0.1 589,630 + +0.1 589,630 + +0.0 589,631 - -0.5 589,631 - +0.1 589,631 - -0.2 589,732 + -1.4 589,774 - -0.2 589,777 - +0.3 589,824 + +1.1 589,824 + +0.2 589,825 - -1.4 589,825 - +1.1 589,898 + -0.6 589,898 + +1.1 589,898 + -0.2 589,899 - +0.1 589,899 - +0.8 589,899 - +0.6 589,919 + +0.1 589,957 - -0.5 589,962 + +0.9 589,963 - -0.3 589,963 - +2.6 589,971 - +0.4 590,019 + -0.5 590,206 - +0.4 590,256 - -0.1 590,256 - -0.5 590,281 + +1.8 590,399 + -0.2 590,442 - -1.3 591,281 - -0.0 591,383 + -0.2 591,768 - +0.1 591,793 + +0.3 591,799 + +0.2 591,800 - -0.5 591,889 + +1.1 591,889 + -0.7 591,889 + -0.2 591,890 - -0.5 591,890 - +1.3 591,890 - -1.5 591,891 + -0.5 591,948 + -0.1 591,949 - +0.3
Or see this region's nucleotide sequence