Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10610 and RPSI07_RS10615 are separated by 348 nucleotides RPSI07_RS10615 and RPSI07_RS10620 are separated by 312 nucleotides RPSI07_RS10620 and RPSI07_RS10625 are separated by 19 nucleotides
RPSI07_RS10610: RPSI07_RS10610 - glucan biosynthesis protein D, at 583,593 to 585,119
_RS10610
RPSI07_RS10615: RPSI07_RS10615 - hypothetical protein, at 585,468 to 586,196
_RS10615
RPSI07_RS10620: RPSI07_RS10620 - UbiX family flavin prenyltransferase, at 586,509 to 587,165
_RS10620
RPSI07_RS10625: RPSI07_RS10625 - monothiol glutaredoxin, Grx4 family, at 587,185 to 587,496
_RS10625
Position (kb) 585
586
587 Strain fitness (log2 ratio) -2
-1
0
1
2 at 584.503 kb on + strand, within RPSI07_RS10610 at 584.504 kb on - strand, within RPSI07_RS10610 at 584.624 kb on - strand, within RPSI07_RS10610 at 584.624 kb on - strand, within RPSI07_RS10610 at 584.858 kb on - strand, within RPSI07_RS10610 at 584.858 kb on - strand, within RPSI07_RS10610 at 584.858 kb on - strand, within RPSI07_RS10610 at 585.229 kb on - strand at 585.250 kb on - strand at 585.712 kb on - strand, within RPSI07_RS10615 at 585.879 kb on + strand, within RPSI07_RS10615 at 586.009 kb on - strand, within RPSI07_RS10615 at 586.201 kb on - strand at 586.217 kb on - strand at 586.227 kb on + strand at 586.228 kb on - strand at 586.228 kb on - strand at 586.236 kb on + strand at 586.237 kb on - strand at 586.272 kb on + strand at 586.273 kb on - strand at 586.273 kb on - strand at 586.275 kb on - strand at 586.372 kb on + strand at 586.408 kb on + strand at 586.409 kb on - strand at 586.429 kb on + strand at 586.432 kb on + strand at 586.433 kb on - strand at 586.461 kb on + strand at 586.461 kb on + strand at 586.461 kb on + strand at 586.462 kb on - strand at 586.465 kb on + strand at 586.469 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 584,503 + RPSI07_RS10610 0.60 +0.5 584,504 - RPSI07_RS10610 0.60 -0.9 584,624 - RPSI07_RS10610 0.68 +0.5 584,624 - RPSI07_RS10610 0.68 -0.1 584,858 - RPSI07_RS10610 0.83 -0.5 584,858 - RPSI07_RS10610 0.83 -0.9 584,858 - RPSI07_RS10610 0.83 +1.1 585,229 - +0.2 585,250 - +0.1 585,712 - RPSI07_RS10615 0.33 -2.5 585,879 + RPSI07_RS10615 0.56 -0.5 586,009 - RPSI07_RS10615 0.74 -2.2 586,201 - +0.3 586,217 - -0.5 586,227 + +0.3 586,228 - -0.3 586,228 - +0.1 586,236 + -2.0 586,237 - -0.6 586,272 + -0.9 586,273 - -0.2 586,273 - -0.0 586,275 - +0.1 586,372 + -0.0 586,408 + +0.4 586,409 - -0.2 586,429 + -0.7 586,432 + +1.1 586,433 - -0.2 586,461 + -0.9 586,461 + -0.1 586,461 + +2.6 586,462 - -0.1 586,465 + -1.5 586,469 - -0.2
Or see this region's nucleotide sequence