Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS01330 and RPSI07_RS01335 are separated by 114 nucleotides RPSI07_RS01335 and RPSI07_RS01340 overlap by 1 nucleotides RPSI07_RS01340 and RPSI07_RS01345 are separated by 366 nucleotides RPSI07_RS01345 and RPSI07_RS01350 are separated by 68 nucleotides
RPSI07_RS01330: RPSI07_RS01330 - xanthine dehydrogenase family protein molybdopterin-binding subunit, at 366,407 to 368,656
_RS01330
RPSI07_RS01335: RPSI07_RS01335 - organic hydroperoxide resistance protein, at 368,771 to 369,205
_RS01335
RPSI07_RS01340: RPSI07_RS01340 - nucleotidyltransferase family protein, at 369,205 to 369,864
_RS01340
RPSI07_RS01345: RPSI07_RS01345 - hypothetical protein, at 370,231 to 370,434
_RS01345
RPSI07_RS01350: RPSI07_RS01350 - GGDEF domain-containing protein, at 370,503 to 373,190
_RS01350
Position (kb) 369
370 Strain fitness (log2 ratio) -1
0
1 at 368.249 kb on + strand, within RPSI07_RS01330 at 368.250 kb on + strand, within RPSI07_RS01330 at 368.250 kb on + strand, within RPSI07_RS01330 at 368.250 kb on + strand, within RPSI07_RS01330 at 368.250 kb on + strand, within RPSI07_RS01330 at 368.251 kb on - strand, within RPSI07_RS01330 at 368.251 kb on - strand, within RPSI07_RS01330 at 368.251 kb on - strand, within RPSI07_RS01330 at 368.279 kb on + strand, within RPSI07_RS01330 at 368.279 kb on + strand, within RPSI07_RS01330 at 368.279 kb on + strand, within RPSI07_RS01330 at 368.279 kb on + strand, within RPSI07_RS01330 at 368.279 kb on + strand, within RPSI07_RS01330 at 368.279 kb on + strand, within RPSI07_RS01330 at 368.279 kb on + strand, within RPSI07_RS01330 at 368.279 kb on + strand, within RPSI07_RS01330 at 368.280 kb on - strand, within RPSI07_RS01330 at 368.280 kb on - strand, within RPSI07_RS01330 at 368.280 kb on - strand, within RPSI07_RS01330 at 368.280 kb on - strand, within RPSI07_RS01330 at 368.280 kb on - strand, within RPSI07_RS01330 at 368.280 kb on - strand, within RPSI07_RS01330 at 368.654 kb on + strand at 368.789 kb on + strand at 368.789 kb on + strand at 368.789 kb on + strand at 368.789 kb on + strand at 368.789 kb on + strand at 368.790 kb on - strand at 368.790 kb on - strand at 368.830 kb on + strand, within RPSI07_RS01335 at 368.831 kb on - strand, within RPSI07_RS01335 at 368.831 kb on - strand, within RPSI07_RS01335 at 369.664 kb on + strand, within RPSI07_RS01340 at 369.664 kb on + strand, within RPSI07_RS01340 at 369.665 kb on - strand, within RPSI07_RS01340 at 369.665 kb on - strand, within RPSI07_RS01340 at 369.733 kb on - strand, within RPSI07_RS01340 at 369.759 kb on + strand, within RPSI07_RS01340 at 369.759 kb on + strand, within RPSI07_RS01340 at 369.759 kb on + strand, within RPSI07_RS01340 at 369.790 kb on - strand, within RPSI07_RS01340 at 369.889 kb on + strand at 369.890 kb on - strand at 369.890 kb on - strand at 370.026 kb on + strand at 370.027 kb on - strand at 370.113 kb on + strand at 370.113 kb on + strand at 370.114 kb on - strand at 370.114 kb on - strand at 370.114 kb on - strand at 370.141 kb on + strand at 370.141 kb on + strand at 370.141 kb on + strand at 370.142 kb on - strand at 370.142 kb on - strand at 370.151 kb on + strand at 370.165 kb on - strand at 370.195 kb on + strand at 370.734 kb on - strand at 370.754 kb on + strand at 370.754 kb on + strand at 370.755 kb on - strand at 370.755 kb on - strand at 370.755 kb on - strand at 370.780 kb on + strand, within RPSI07_RS01350 at 370.780 kb on + strand, within RPSI07_RS01350 at 370.781 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 368,249 + RPSI07_RS01330 0.82 +1.0 368,250 + RPSI07_RS01330 0.82 -0.3 368,250 + RPSI07_RS01330 0.82 -1.0 368,250 + RPSI07_RS01330 0.82 +0.8 368,250 + RPSI07_RS01330 0.82 +0.4 368,251 - RPSI07_RS01330 0.82 -1.0 368,251 - RPSI07_RS01330 0.82 -0.2 368,251 - RPSI07_RS01330 0.82 -0.6 368,279 + RPSI07_RS01330 0.83 -0.2 368,279 + RPSI07_RS01330 0.83 -1.0 368,279 + RPSI07_RS01330 0.83 +0.0 368,279 + RPSI07_RS01330 0.83 -0.2 368,279 + RPSI07_RS01330 0.83 +0.2 368,279 + RPSI07_RS01330 0.83 -0.6 368,279 + RPSI07_RS01330 0.83 +0.0 368,279 + RPSI07_RS01330 0.83 -1.0 368,280 - RPSI07_RS01330 0.83 +0.7 368,280 - RPSI07_RS01330 0.83 +0.0 368,280 - RPSI07_RS01330 0.83 +0.1 368,280 - RPSI07_RS01330 0.83 +1.0 368,280 - RPSI07_RS01330 0.83 -0.7 368,280 - RPSI07_RS01330 0.83 -0.2 368,654 + -0.2 368,789 + -0.9 368,789 + -1.0 368,789 + +1.3 368,789 + -1.6 368,789 + -1.7 368,790 - -0.6 368,790 - -0.3 368,830 + RPSI07_RS01335 0.14 +0.4 368,831 - RPSI07_RS01335 0.14 +1.2 368,831 - RPSI07_RS01335 0.14 -0.3 369,664 + RPSI07_RS01340 0.70 +0.0 369,664 + RPSI07_RS01340 0.70 -0.6 369,665 - RPSI07_RS01340 0.70 +0.0 369,665 - RPSI07_RS01340 0.70 -0.1 369,733 - RPSI07_RS01340 0.80 -0.5 369,759 + RPSI07_RS01340 0.84 -1.0 369,759 + RPSI07_RS01340 0.84 -0.4 369,759 + RPSI07_RS01340 0.84 -1.0 369,790 - RPSI07_RS01340 0.89 -0.6 369,889 + -0.6 369,890 - +0.2 369,890 - -0.7 370,026 + +0.0 370,027 - +1.6 370,113 + +1.0 370,113 + +0.0 370,114 - +1.0 370,114 - +0.4 370,114 - -0.1 370,141 + +0.6 370,141 + +0.6 370,141 + +0.0 370,142 - +0.7 370,142 - +0.0 370,151 + -0.1 370,165 - -0.7 370,195 + +0.6 370,734 - +0.3 370,754 + +0.8 370,754 + +0.0 370,755 - +0.1 370,755 - -0.0 370,755 - +0.0 370,780 + RPSI07_RS01350 0.10 -1.3 370,780 + RPSI07_RS01350 0.10 +0.0 370,781 - +0.0
Or see this region's nucleotide sequence