Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS00520 and RPSI07_RS00525 are separated by 238 nucleotides RPSI07_RS00525 and RPSI07_RS00530 are separated by 149 nucleotides RPSI07_RS00530 and RPSI07_RS00535 are separated by 326 nucleotides
RPSI07_RS00520: RPSI07_RS00520 - hypothetical protein, at 138,778 to 139,413
_RS00520
RPSI07_RS00525: RPSI07_RS00525 - hypothetical protein, at 139,652 to 139,999
_RS00525
RPSI07_RS00530: RPSI07_RS00530 - hypothetical protein, at 140,149 to 140,946
_RS00530
RPSI07_RS00535: RPSI07_RS00535 - hypothetical protein, at 141,273 to 141,563
_RS00535
Position (kb)
140
141 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 139.186 kb on + strand, within RPSI07_RS00520 at 139.186 kb on + strand, within RPSI07_RS00520 at 139.187 kb on - strand, within RPSI07_RS00520 at 139.187 kb on - strand, within RPSI07_RS00520 at 139.187 kb on - strand, within RPSI07_RS00520 at 139.187 kb on - strand, within RPSI07_RS00520 at 139.250 kb on - strand, within RPSI07_RS00520 at 139.271 kb on - strand, within RPSI07_RS00520 at 139.271 kb on - strand, within RPSI07_RS00520 at 139.409 kb on + strand at 139.409 kb on + strand at 139.409 kb on + strand at 139.410 kb on - strand at 139.410 kb on - strand at 139.410 kb on - strand at 139.410 kb on - strand at 139.410 kb on - strand at 139.410 kb on - strand at 139.410 kb on - strand at 139.411 kb on + strand at 139.411 kb on + strand at 139.412 kb on - strand at 139.412 kb on - strand at 139.576 kb on + strand at 139.576 kb on + strand at 139.576 kb on + strand at 139.576 kb on + strand at 139.576 kb on + strand at 139.577 kb on - strand at 139.577 kb on - strand at 139.577 kb on - strand at 139.598 kb on + strand at 139.598 kb on + strand at 139.598 kb on + strand at 139.598 kb on + strand at 139.598 kb on + strand at 139.598 kb on + strand at 139.598 kb on + strand at 139.598 kb on + strand at 139.599 kb on - strand at 139.599 kb on - strand at 139.599 kb on - strand at 139.599 kb on - strand at 139.638 kb on + strand at 139.639 kb on - strand at 139.639 kb on - strand at 139.898 kb on + strand, within RPSI07_RS00525 at 139.898 kb on + strand, within RPSI07_RS00525 at 139.899 kb on - strand, within RPSI07_RS00525 at 139.899 kb on - strand, within RPSI07_RS00525 at 139.900 kb on - strand, within RPSI07_RS00525 at 140.297 kb on + strand, within RPSI07_RS00530 at 140.298 kb on - strand, within RPSI07_RS00530 at 140.309 kb on + strand, within RPSI07_RS00530 at 140.310 kb on - strand, within RPSI07_RS00530 at 140.310 kb on - strand, within RPSI07_RS00530 at 140.514 kb on - strand, within RPSI07_RS00530 at 140.514 kb on - strand, within RPSI07_RS00530 at 140.645 kb on - strand, within RPSI07_RS00530 at 140.645 kb on - strand, within RPSI07_RS00530 at 140.849 kb on + strand, within RPSI07_RS00530 at 140.913 kb on - strand at 140.913 kb on - strand at 140.913 kb on - strand at 140.988 kb on - strand at 141.157 kb on + strand at 141.157 kb on + strand at 141.158 kb on - strand at 141.245 kb on - strand at 141.245 kb on - strand at 141.245 kb on - strand at 141.285 kb on + strand at 141.285 kb on + strand at 141.285 kb on + strand at 141.285 kb on + strand at 141.285 kb on + strand at 141.286 kb on - strand at 141.286 kb on - strand at 141.286 kb on - strand at 141.512 kb on + strand, within RPSI07_RS00535 at 141.512 kb on + strand, within RPSI07_RS00535 at 141.512 kb on + strand, within RPSI07_RS00535 at 141.512 kb on + strand, within RPSI07_RS00535 at 141.512 kb on + strand, within RPSI07_RS00535 at 141.512 kb on + strand, within RPSI07_RS00535 at 141.512 kb on + strand, within RPSI07_RS00535 at 141.513 kb on - strand, within RPSI07_RS00535 at 141.513 kb on - strand, within RPSI07_RS00535 at 141.513 kb on - strand, within RPSI07_RS00535 at 141.513 kb on - strand, within RPSI07_RS00535 at 141.513 kb on - strand, within RPSI07_RS00535 at 141.513 kb on - strand, within RPSI07_RS00535 at 141.664 kb on + strand at 141.664 kb on + strand at 141.706 kb on + strand at 141.706 kb on + strand at 141.706 kb on + strand at 141.706 kb on + strand at 141.706 kb on + strand at 141.707 kb on - strand at 141.707 kb on - strand at 141.707 kb on - strand at 141.707 kb on - strand at 141.728 kb on + strand at 141.728 kb on + strand at 141.728 kb on + strand at 141.733 kb on + strand at 141.771 kb on - strand at 141.771 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 139,186 + RPSI07_RS00520 0.64 -1.6 139,186 + RPSI07_RS00520 0.64 -1.0 139,187 - RPSI07_RS00520 0.64 +1.0 139,187 - RPSI07_RS00520 0.64 -0.3 139,187 - RPSI07_RS00520 0.64 +0.2 139,187 - RPSI07_RS00520 0.64 +0.1 139,250 - RPSI07_RS00520 0.74 -1.4 139,271 - RPSI07_RS00520 0.78 -1.2 139,271 - RPSI07_RS00520 0.78 -0.0 139,409 + -0.0 139,409 + -0.6 139,409 + +0.5 139,410 - -0.8 139,410 - +0.1 139,410 - -1.1 139,410 - +0.0 139,410 - -1.3 139,410 - -0.6 139,410 - +1.0 139,411 + +0.4 139,411 + -1.3 139,412 - -1.1 139,412 - +1.0 139,576 + +0.0 139,576 + -1.8 139,576 + -0.7 139,576 + +0.2 139,576 + -0.4 139,577 - +0.6 139,577 - -0.5 139,577 - +0.5 139,598 + +0.7 139,598 + +0.4 139,598 + +0.2 139,598 + -0.4 139,598 + -0.3 139,598 + -0.6 139,598 + -0.4 139,598 + -0.8 139,599 - +1.6 139,599 - +0.3 139,599 - +0.0 139,599 - +1.0 139,638 + -0.2 139,639 - -0.1 139,639 - -0.7 139,898 + RPSI07_RS00525 0.71 -0.8 139,898 + RPSI07_RS00525 0.71 -0.2 139,899 - RPSI07_RS00525 0.71 -0.7 139,899 - RPSI07_RS00525 0.71 -1.1 139,900 - RPSI07_RS00525 0.71 +0.0 140,297 + RPSI07_RS00530 0.19 +0.6 140,298 - RPSI07_RS00530 0.19 -0.5 140,309 + RPSI07_RS00530 0.20 -1.2 140,310 - RPSI07_RS00530 0.20 -1.3 140,310 - RPSI07_RS00530 0.20 +0.0 140,514 - RPSI07_RS00530 0.46 -0.6 140,514 - RPSI07_RS00530 0.46 -1.0 140,645 - RPSI07_RS00530 0.62 -0.2 140,645 - RPSI07_RS00530 0.62 -1.0 140,849 + RPSI07_RS00530 0.88 +0.0 140,913 - +0.3 140,913 - +1.0 140,913 - -0.6 140,988 - -0.3 141,157 + +0.4 141,157 + +2.6 141,158 - -0.1 141,245 - -0.3 141,245 - +0.8 141,245 - +0.0 141,285 + +0.2 141,285 + +0.2 141,285 + -0.3 141,285 + -0.5 141,285 + -0.9 141,286 - -0.4 141,286 - -0.2 141,286 - -0.6 141,512 + RPSI07_RS00535 0.82 +0.2 141,512 + RPSI07_RS00535 0.82 -0.1 141,512 + RPSI07_RS00535 0.82 +1.3 141,512 + RPSI07_RS00535 0.82 +2.0 141,512 + RPSI07_RS00535 0.82 -0.6 141,512 + RPSI07_RS00535 0.82 -0.9 141,512 + RPSI07_RS00535 0.82 +0.1 141,513 - RPSI07_RS00535 0.82 -0.7 141,513 - RPSI07_RS00535 0.82 -0.2 141,513 - RPSI07_RS00535 0.82 -0.8 141,513 - RPSI07_RS00535 0.82 -0.7 141,513 - RPSI07_RS00535 0.82 -0.3 141,513 - RPSI07_RS00535 0.82 -0.5 141,664 + -0.5 141,664 + -0.1 141,706 + +0.5 141,706 + -0.5 141,706 + -1.3 141,706 + -0.1 141,706 + -1.6 141,707 - +0.0 141,707 - -0.5 141,707 - -0.4 141,707 - +0.7 141,728 + -0.1 141,728 + +1.0 141,728 + +1.0 141,733 + +3.3 141,771 - -1.0 141,771 - +0.3
Or see this region's nucleotide sequence