Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS15690

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS15685 and RPSI07_RS15690 are separated by 22 nucleotidesRPSI07_RS15690 and RPSI07_RS15695 are separated by 364 nucleotides RPSI07_RS15685: RPSI07_RS15685 - bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 1,677,487 to 1,679,727 _RS15685 RPSI07_RS15690: RPSI07_RS15690 - tRNA-Val, at 1,679,750 to 1,679,826 _RS15690 RPSI07_RS15695: RPSI07_RS15695 - threonine--tRNA ligase, at 1,680,191 to 1,682,098 _RS15695 Position (kb) 1679 1680Strain fitness (log2 ratio) -1 0 1 2at 1678.966 kb on + strand, within RPSI07_RS15685at 1678.967 kb on - strand, within RPSI07_RS15685at 1678.967 kb on - strand, within RPSI07_RS15685at 1678.967 kb on - strand, within RPSI07_RS15685at 1678.967 kb on - strand, within RPSI07_RS15685at 1678.990 kb on - strand, within RPSI07_RS15685at 1678.990 kb on - strand, within RPSI07_RS15685at 1679.146 kb on + strand, within RPSI07_RS15685at 1679.501 kb on - strand, within RPSI07_RS15685at 1679.685 kb on - strandat 1679.888 kb on + strandat 1679.898 kb on + strandat 1679.930 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
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1,678,966 + RPSI07_RS15685 0.66 +0.3
1,678,967 - RPSI07_RS15685 0.66 -0.2
1,678,967 - RPSI07_RS15685 0.66 -1.0
1,678,967 - RPSI07_RS15685 0.66 +1.8
1,678,967 - RPSI07_RS15685 0.66 +0.5
1,678,990 - RPSI07_RS15685 0.67 +0.3
1,678,990 - RPSI07_RS15685 0.67 -0.0
1,679,146 + RPSI07_RS15685 0.74 -0.1
1,679,501 - RPSI07_RS15685 0.90 +0.1
1,679,685 - -0.2
1,679,888 + +0.8
1,679,898 + +0.3
1,679,930 + +0.8

Or see this region's nucleotide sequence