Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS12050 and RPSI07_RS12055 overlap by 4 nucleotides RPSI07_RS12055 and RPSI07_RS12060 are separated by 101 nucleotides RPSI07_RS12060 and RPSI07_RS12065 overlap by 4 nucleotides RPSI07_RS12065 and RPSI07_RS12070 are separated by 7 nucleotides
RPSI07_RS12050: RPSI07_RS12050 - prolyl 4-hydroxylase, at 906,890 to 907,624
_RS12050
RPSI07_RS12055: RPSI07_RS12055 - cysteine methyltransferase, at 907,621 to 908,136
_RS12055
RPSI07_RS12060: RPSI07_RS12060 - N-acetyltransferase, at 908,238 to 908,771
_RS12060
RPSI07_RS12065: RPSI07_RS12065 - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 908,768 to 909,739
_RS12065
RPSI07_RS12070: RPSI07_RS12070 - DNA mismatch repair endonuclease MutL, at 909,747 to 911,660
_RS12070
Position (kb) 908
909 Strain fitness (log2 ratio) -2
-1
0
1 at 907.347 kb on + strand, within RPSI07_RS12050 at 907.399 kb on - strand, within RPSI07_RS12050 at 907.399 kb on - strand, within RPSI07_RS12050 at 907.404 kb on + strand, within RPSI07_RS12050 at 907.405 kb on - strand, within RPSI07_RS12050 at 907.470 kb on + strand, within RPSI07_RS12050 at 907.676 kb on + strand, within RPSI07_RS12055 at 907.809 kb on - strand, within RPSI07_RS12055 at 908.075 kb on - strand, within RPSI07_RS12055 at 908.105 kb on + strand at 908.106 kb on - strand at 908.106 kb on - strand at 908.106 kb on - strand at 908.354 kb on - strand, within RPSI07_RS12060 at 908.504 kb on - strand, within RPSI07_RS12060 at 908.504 kb on - strand, within RPSI07_RS12060 at 908.599 kb on + strand, within RPSI07_RS12060 at 908.952 kb on + strand, within RPSI07_RS12065 at 908.952 kb on + strand, within RPSI07_RS12065 at 909.133 kb on - strand, within RPSI07_RS12065 at 909.133 kb on - strand, within RPSI07_RS12065 at 909.411 kb on + strand, within RPSI07_RS12065
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 907,347 + RPSI07_RS12050 0.62 -0.0 907,399 - RPSI07_RS12050 0.69 +0.1 907,399 - RPSI07_RS12050 0.69 -0.2 907,404 + RPSI07_RS12050 0.70 -1.3 907,405 - RPSI07_RS12050 0.70 +0.1 907,470 + RPSI07_RS12050 0.79 -0.1 907,676 + RPSI07_RS12055 0.11 +0.0 907,809 - RPSI07_RS12055 0.36 +0.0 908,075 - RPSI07_RS12055 0.88 +1.0 908,105 + +1.0 908,106 - +0.0 908,106 - -0.1 908,106 - -0.5 908,354 - RPSI07_RS12060 0.22 -0.2 908,504 - RPSI07_RS12060 0.50 -0.6 908,504 - RPSI07_RS12060 0.50 -0.6 908,599 + RPSI07_RS12060 0.68 +0.4 908,952 + RPSI07_RS12065 0.19 -1.9 908,952 + RPSI07_RS12065 0.19 -0.7 909,133 - RPSI07_RS12065 0.38 +1.0 909,133 - RPSI07_RS12065 0.38 -1.0 909,411 + RPSI07_RS12065 0.66 -0.0
Or see this region's nucleotide sequence