Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS22435

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS22425 and RPSI07_RS22430 are separated by 139 nucleotidesRPSI07_RS22430 and RPSI07_RS22435 are separated by 210 nucleotidesRPSI07_RS22435 and RPSI07_RS22440 are separated by 40 nucleotidesRPSI07_RS22440 and RPSI07_RS22445 overlap by 32 nucleotidesRPSI07_RS22445 and RPSI07_RS22450 are separated by 108 nucleotides RPSI07_RS22425: RPSI07_RS22425 - bacterioferritin, at 3,188,589 to 3,189,068 _RS22425 RPSI07_RS22430: RPSI07_RS22430 - (2Fe-2S)-binding protein, at 3,189,208 to 3,189,483 _RS22430 RPSI07_RS22435: RPSI07_RS22435 - phosphoenolpyruvate--protein phosphotransferase, at 3,189,694 to 3,191,454 _RS22435 RPSI07_RS22440: RPSI07_RS22440 - HPr family phosphocarrier protein, at 3,191,495 to 3,191,764 _RS22440 RPSI07_RS22445: RPSI07_RS22445 - PTS fructose transporter subunit IIA, at 3,191,733 to 3,192,188 _RS22445 RPSI07_RS22450: RPSI07_RS22450 - glutathione synthase, at 3,192,297 to 3,193,271 _RS22450 Position (kb) 3189 3190 3191 3192Strain fitness (log2 ratio) -2 -1 0 1at 3188.728 kb on + strand, within RPSI07_RS22425at 3188.728 kb on + strand, within RPSI07_RS22425at 3188.728 kb on + strand, within RPSI07_RS22425at 3188.750 kb on - strand, within RPSI07_RS22425at 3188.750 kb on - strand, within RPSI07_RS22425at 3188.935 kb on + strand, within RPSI07_RS22425at 3188.935 kb on + strand, within RPSI07_RS22425at 3188.935 kb on + strand, within RPSI07_RS22425at 3188.935 kb on + strand, within RPSI07_RS22425at 3188.936 kb on - strand, within RPSI07_RS22425at 3188.936 kb on - strand, within RPSI07_RS22425at 3188.974 kb on + strand, within RPSI07_RS22425at 3188.974 kb on + strand, within RPSI07_RS22425at 3188.974 kb on + strand, within RPSI07_RS22425at 3188.975 kb on - strand, within RPSI07_RS22425at 3188.975 kb on - strand, within RPSI07_RS22425at 3188.975 kb on - strand, within RPSI07_RS22425at 3188.975 kb on - strand, within RPSI07_RS22425at 3188.975 kb on - strand, within RPSI07_RS22425at 3188.995 kb on + strand, within RPSI07_RS22425at 3188.995 kb on + strand, within RPSI07_RS22425at 3188.996 kb on - strand, within RPSI07_RS22425at 3188.996 kb on - strand, within RPSI07_RS22425at 3189.040 kb on + strandat 3189.056 kb on + strandat 3189.057 kb on - strandat 3189.274 kb on + strand, within RPSI07_RS22430at 3189.274 kb on + strand, within RPSI07_RS22430at 3189.275 kb on - strand, within RPSI07_RS22430at 3189.479 kb on + strandat 3189.479 kb on + strandat 3189.479 kb on + strandat 3189.479 kb on + strandat 3189.480 kb on - strandat 3189.480 kb on - strandat 3189.480 kb on - strandat 3189.480 kb on - strandat 3189.528 kb on - strandat 3189.990 kb on - strand, within RPSI07_RS22435at 3190.034 kb on + strand, within RPSI07_RS22435at 3190.152 kb on - strand, within RPSI07_RS22435at 3190.152 kb on - strand, within RPSI07_RS22435at 3190.152 kb on - strand, within RPSI07_RS22435at 3190.712 kb on + strand, within RPSI07_RS22435at 3191.081 kb on + strand, within RPSI07_RS22435at 3191.153 kb on + strand, within RPSI07_RS22435at 3191.361 kb on - strandat 3191.361 kb on - strandat 3191.361 kb on - strandat 3191.394 kb on + strandat 3191.395 kb on - strandat 3191.395 kb on - strandat 3191.445 kb on + strandat 3191.446 kb on - strandat 3191.446 kb on - strandat 3191.465 kb on + strandat 3191.466 kb on - strandat 3191.496 kb on + strandat 3191.734 kb on + strandat 3191.734 kb on + strandat 3191.734 kb on + strandat 3191.735 kb on - strandat 3191.749 kb on + strandat 3191.750 kb on - strandat 3191.750 kb on - strandat 3191.750 kb on - strandat 3191.764 kb on + strandat 3191.764 kb on + strandat 3191.764 kb on + strandat 3191.764 kb on + strandat 3191.764 kb on + strandat 3191.765 kb on - strandat 3191.765 kb on - strandat 3191.765 kb on - strandat 3191.765 kb on - strandat 3191.765 kb on - strandat 3191.820 kb on + strand, within RPSI07_RS22445at 3192.118 kb on + strand, within RPSI07_RS22445at 3192.118 kb on + strand, within RPSI07_RS22445at 3192.118 kb on + strand, within RPSI07_RS22445at 3192.221 kb on + strandat 3192.221 kb on + strandat 3192.221 kb on + strandat 3192.221 kb on + strandat 3192.222 kb on - strandat 3192.222 kb on - strandat 3192.222 kb on - strandat 3192.273 kb on + strandat 3192.299 kb on - strandat 3192.299 kb on - strandat 3192.442 kb on + strand, within RPSI07_RS22450at 3192.442 kb on + strand, within RPSI07_RS22450

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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3,188,728 + RPSI07_RS22425 0.29 -0.1
3,188,728 + RPSI07_RS22425 0.29 -0.0
3,188,728 + RPSI07_RS22425 0.29 +0.0
3,188,750 - RPSI07_RS22425 0.34 -0.7
3,188,750 - RPSI07_RS22425 0.34 -0.5
3,188,935 + RPSI07_RS22425 0.72 +0.8
3,188,935 + RPSI07_RS22425 0.72 +0.0
3,188,935 + RPSI07_RS22425 0.72 -1.2
3,188,935 + RPSI07_RS22425 0.72 +0.5
3,188,936 - RPSI07_RS22425 0.72 +0.2
3,188,936 - RPSI07_RS22425 0.72 +0.8
3,188,974 + RPSI07_RS22425 0.80 +0.6
3,188,974 + RPSI07_RS22425 0.80 -0.6
3,188,974 + RPSI07_RS22425 0.80 -0.2
3,188,975 - RPSI07_RS22425 0.80 +0.8
3,188,975 - RPSI07_RS22425 0.80 -1.5
3,188,975 - RPSI07_RS22425 0.80 +1.2
3,188,975 - RPSI07_RS22425 0.80 +0.2
3,188,975 - RPSI07_RS22425 0.80 +0.3
3,188,995 + RPSI07_RS22425 0.85 -0.1
3,188,995 + RPSI07_RS22425 0.85 -0.7
3,188,996 - RPSI07_RS22425 0.85 +0.4
3,188,996 - RPSI07_RS22425 0.85 +0.8
3,189,040 + +0.7
3,189,056 + +0.3
3,189,057 - +0.0
3,189,274 + RPSI07_RS22430 0.24 -0.2
3,189,274 + RPSI07_RS22430 0.24 +0.1
3,189,275 - RPSI07_RS22430 0.24 +0.2
3,189,479 + -1.8
3,189,479 + -0.8
3,189,479 + -1.0
3,189,479 + +1.4
3,189,480 - +0.4
3,189,480 - -0.5
3,189,480 - +0.2
3,189,480 - +0.2
3,189,528 - -1.4
3,189,990 - RPSI07_RS22435 0.17 -0.8
3,190,034 + RPSI07_RS22435 0.19 -1.8
3,190,152 - RPSI07_RS22435 0.26 -2.2
3,190,152 - RPSI07_RS22435 0.26 -1.8
3,190,152 - RPSI07_RS22435 0.26 -1.4
3,190,712 + RPSI07_RS22435 0.58 +0.8
3,191,081 + RPSI07_RS22435 0.79 -1.3
3,191,153 + RPSI07_RS22435 0.83 -1.6
3,191,361 - +0.8
3,191,361 - -1.3
3,191,361 - -1.3
3,191,394 + -2.2
3,191,395 - -0.6
3,191,395 - -1.0
3,191,445 + -2.0
3,191,446 - -1.9
3,191,446 - -0.7
3,191,465 + -1.6
3,191,466 - -0.1
3,191,496 + -2.2
3,191,734 + -1.0
3,191,734 + -0.4
3,191,734 + -1.0
3,191,735 - +0.7
3,191,749 + -0.5
3,191,750 - -0.2
3,191,750 - +0.4
3,191,750 - +0.8
3,191,764 + -0.5
3,191,764 + +0.8
3,191,764 + -0.9
3,191,764 + -0.5
3,191,764 + +0.1
3,191,765 - +0.2
3,191,765 - -0.3
3,191,765 - +0.6
3,191,765 - -0.2
3,191,765 - -0.8
3,191,820 + RPSI07_RS22445 0.19 -0.3
3,192,118 + RPSI07_RS22445 0.84 -1.5
3,192,118 + RPSI07_RS22445 0.84 +1.4
3,192,118 + RPSI07_RS22445 0.84 -0.8
3,192,221 + -1.3
3,192,221 + +0.1
3,192,221 + -0.7
3,192,221 + +0.8
3,192,222 - -0.1
3,192,222 - -1.6
3,192,222 - -0.4
3,192,273 + -0.1
3,192,299 - -1.0
3,192,299 - +0.0
3,192,442 + RPSI07_RS22450 0.15 -1.4
3,192,442 + RPSI07_RS22450 0.15 -1.9

Or see this region's nucleotide sequence