Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS22330 and RPSI07_RS22335 are separated by 55 nucleotides RPSI07_RS22335 and RPSI07_RS22340 are separated by 81 nucleotides RPSI07_RS22340 and RPSI07_RS22345 are separated by 81 nucleotides RPSI07_RS22345 and RPSI07_RS22350 are separated by 4 nucleotides RPSI07_RS22350 and RPSI07_RS22355 are separated by 34 nucleotides
RPSI07_RS22330: RPSI07_RS22330 - SURF1 family protein, at 3,173,425 to 3,174,195
_RS22330
RPSI07_RS22335: RPSI07_RS22335 - twin transmembrane helix small protein, at 3,174,251 to 3,174,460
_RS22335
RPSI07_RS22340: RPSI07_RS22340 - cytochrome c oxidase subunit 3, at 3,174,542 to 3,175,402
_RS22340
RPSI07_RS22345: RPSI07_RS22345 - DUF2970 domain-containing protein, at 3,175,484 to 3,175,696
_RS22345
RPSI07_RS22350: RPSI07_RS22350 - cytochrome c oxidase assembly protein, at 3,175,701 to 3,176,309
_RS22350
RPSI07_RS22355: RPSI07_RS22355 - hypothetical protein, at 3,176,344 to 3,176,538
_RS22355
Position (kb)
3174
3175
3176 Strain fitness (log2 ratio)
-2
-1
0
1 at 3173.665 kb on - strand, within RPSI07_RS22330 at 3173.673 kb on + strand, within RPSI07_RS22330 at 3173.674 kb on - strand, within RPSI07_RS22330 at 3173.674 kb on - strand, within RPSI07_RS22330 at 3173.719 kb on - strand, within RPSI07_RS22330 at 3173.783 kb on + strand, within RPSI07_RS22330 at 3173.784 kb on - strand, within RPSI07_RS22330 at 3173.919 kb on + strand, within RPSI07_RS22330 at 3173.920 kb on - strand, within RPSI07_RS22330 at 3173.945 kb on + strand, within RPSI07_RS22330 at 3173.946 kb on + strand, within RPSI07_RS22330 at 3173.988 kb on - strand, within RPSI07_RS22330 at 3173.988 kb on - strand, within RPSI07_RS22330 at 3173.988 kb on - strand, within RPSI07_RS22330 at 3174.175 kb on + strand at 3174.175 kb on + strand at 3174.214 kb on + strand at 3174.214 kb on + strand at 3174.544 kb on - strand at 3174.544 kb on - strand at 3174.552 kb on + strand at 3174.553 kb on - strand at 3174.564 kb on + strand at 3174.565 kb on - strand at 3174.565 kb on - strand at 3174.565 kb on - strand at 3174.744 kb on + strand, within RPSI07_RS22340 at 3174.744 kb on + strand, within RPSI07_RS22340 at 3174.745 kb on - strand, within RPSI07_RS22340 at 3174.745 kb on - strand, within RPSI07_RS22340 at 3174.745 kb on - strand, within RPSI07_RS22340 at 3174.745 kb on - strand, within RPSI07_RS22340 at 3174.781 kb on - strand, within RPSI07_RS22340 at 3174.792 kb on + strand, within RPSI07_RS22340 at 3174.792 kb on + strand, within RPSI07_RS22340 at 3174.792 kb on + strand, within RPSI07_RS22340 at 3174.792 kb on + strand, within RPSI07_RS22340 at 3174.792 kb on + strand, within RPSI07_RS22340 at 3174.792 kb on + strand, within RPSI07_RS22340 at 3174.793 kb on - strand, within RPSI07_RS22340 at 3174.793 kb on - strand, within RPSI07_RS22340 at 3174.793 kb on - strand, within RPSI07_RS22340 at 3174.793 kb on - strand, within RPSI07_RS22340 at 3174.793 kb on - strand, within RPSI07_RS22340 at 3174.793 kb on - strand, within RPSI07_RS22340 at 3174.799 kb on - strand, within RPSI07_RS22340 at 3174.799 kb on - strand, within RPSI07_RS22340 at 3174.799 kb on - strand, within RPSI07_RS22340 at 3175.083 kb on + strand, within RPSI07_RS22340 at 3175.084 kb on - strand, within RPSI07_RS22340 at 3175.179 kb on + strand, within RPSI07_RS22340 at 3175.179 kb on + strand, within RPSI07_RS22340 at 3175.180 kb on - strand, within RPSI07_RS22340 at 3175.180 kb on - strand, within RPSI07_RS22340 at 3175.371 kb on + strand at 3175.371 kb on + strand at 3175.372 kb on - strand at 3175.424 kb on - strand at 3175.766 kb on - strand, within RPSI07_RS22350 at 3175.973 kb on - strand, within RPSI07_RS22350 at 3176.096 kb on + strand, within RPSI07_RS22350 at 3176.170 kb on + strand, within RPSI07_RS22350 at 3176.400 kb on + strand, within RPSI07_RS22355
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 3,173,665 - RPSI07_RS22330 0.31 -0.1 3,173,673 + RPSI07_RS22330 0.32 +0.8 3,173,674 - RPSI07_RS22330 0.32 -1.0 3,173,674 - RPSI07_RS22330 0.32 -0.6 3,173,719 - RPSI07_RS22330 0.38 -1.2 3,173,783 + RPSI07_RS22330 0.46 -0.2 3,173,784 - RPSI07_RS22330 0.47 -1.1 3,173,919 + RPSI07_RS22330 0.64 -2.0 3,173,920 - RPSI07_RS22330 0.64 -1.7 3,173,945 + RPSI07_RS22330 0.67 -0.3 3,173,946 + RPSI07_RS22330 0.68 -1.1 3,173,988 - RPSI07_RS22330 0.73 -1.2 3,173,988 - RPSI07_RS22330 0.73 -2.5 3,173,988 - RPSI07_RS22330 0.73 -1.0 3,174,175 + +1.1 3,174,175 + -1.3 3,174,214 + -1.0 3,174,214 + -1.2 3,174,544 - -0.2 3,174,544 - +0.2 3,174,552 + -0.8 3,174,553 - +0.8 3,174,564 + -0.7 3,174,565 - -0.9 3,174,565 - -0.7 3,174,565 - -0.6 3,174,744 + RPSI07_RS22340 0.23 -0.3 3,174,744 + RPSI07_RS22340 0.23 -1.1 3,174,745 - RPSI07_RS22340 0.24 -0.9 3,174,745 - RPSI07_RS22340 0.24 -1.8 3,174,745 - RPSI07_RS22340 0.24 -0.6 3,174,745 - RPSI07_RS22340 0.24 -0.6 3,174,781 - RPSI07_RS22340 0.28 -1.2 3,174,792 + RPSI07_RS22340 0.29 -0.8 3,174,792 + RPSI07_RS22340 0.29 -1.1 3,174,792 + RPSI07_RS22340 0.29 -0.5 3,174,792 + RPSI07_RS22340 0.29 -0.8 3,174,792 + RPSI07_RS22340 0.29 -0.2 3,174,792 + RPSI07_RS22340 0.29 +0.2 3,174,793 - RPSI07_RS22340 0.29 -0.2 3,174,793 - RPSI07_RS22340 0.29 +0.8 3,174,793 - RPSI07_RS22340 0.29 -0.6 3,174,793 - RPSI07_RS22340 0.29 -2.0 3,174,793 - RPSI07_RS22340 0.29 -0.2 3,174,793 - RPSI07_RS22340 0.29 -0.0 3,174,799 - RPSI07_RS22340 0.30 -0.1 3,174,799 - RPSI07_RS22340 0.30 -0.9 3,174,799 - RPSI07_RS22340 0.30 +0.3 3,175,083 + RPSI07_RS22340 0.63 -1.5 3,175,084 - RPSI07_RS22340 0.63 -0.4 3,175,179 + RPSI07_RS22340 0.74 +0.2 3,175,179 + RPSI07_RS22340 0.74 -0.3 3,175,180 - RPSI07_RS22340 0.74 -0.2 3,175,180 - RPSI07_RS22340 0.74 -0.2 3,175,371 + -1.3 3,175,371 + -0.8 3,175,372 - -0.2 3,175,424 - +0.2 3,175,766 - RPSI07_RS22350 0.11 -1.1 3,175,973 - RPSI07_RS22350 0.45 -0.3 3,176,096 + RPSI07_RS22350 0.65 -0.7 3,176,170 + RPSI07_RS22350 0.77 -0.8 3,176,400 + RPSI07_RS22355 0.29 -0.4
Or see this region's nucleotide sequence