Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21670 and RPSI07_RS21675 are separated by 105 nucleotides RPSI07_RS21675 and RPSI07_RS21680 are separated by 68 nucleotides
RPSI07_RS21670: RPSI07_RS21670 - ABC transporter ATP-binding protein/permease, at 3,020,186 to 3,022,012
_RS21670
RPSI07_RS21675: RPSI07_RS21675 - acyl-CoA thioesterase, at 3,022,118 to 3,022,537
_RS21675
RPSI07_RS21680: RPSI07_RS21680 - enoyl-CoA hydratase, at 3,022,606 to 3,023,370
_RS21680
Position (kb)
3022
3023 Strain fitness (log2 ratio)
-1
0
1
2 at 3021.159 kb on + strand, within RPSI07_RS21670 at 3021.288 kb on + strand, within RPSI07_RS21670 at 3021.307 kb on - strand, within RPSI07_RS21670 at 3021.307 kb on - strand, within RPSI07_RS21670 at 3021.429 kb on + strand, within RPSI07_RS21670 at 3021.430 kb on - strand, within RPSI07_RS21670 at 3021.430 kb on - strand, within RPSI07_RS21670 at 3021.430 kb on - strand, within RPSI07_RS21670 at 3021.430 kb on - strand, within RPSI07_RS21670 at 3021.430 kb on - strand, within RPSI07_RS21670 at 3021.430 kb on - strand, within RPSI07_RS21670 at 3021.474 kb on + strand, within RPSI07_RS21670 at 3021.474 kb on + strand, within RPSI07_RS21670 at 3021.474 kb on + strand, within RPSI07_RS21670 at 3021.474 kb on + strand, within RPSI07_RS21670 at 3021.475 kb on - strand, within RPSI07_RS21670 at 3021.475 kb on - strand, within RPSI07_RS21670 at 3021.489 kb on + strand, within RPSI07_RS21670 at 3021.489 kb on + strand, within RPSI07_RS21670 at 3021.489 kb on + strand, within RPSI07_RS21670 at 3021.489 kb on + strand, within RPSI07_RS21670 at 3021.490 kb on - strand, within RPSI07_RS21670 at 3021.490 kb on - strand, within RPSI07_RS21670 at 3021.490 kb on - strand, within RPSI07_RS21670 at 3021.490 kb on - strand, within RPSI07_RS21670 at 3021.534 kb on + strand, within RPSI07_RS21670 at 3021.544 kb on - strand, within RPSI07_RS21670 at 3021.912 kb on + strand at 3021.912 kb on + strand at 3021.913 kb on - strand at 3021.913 kb on - strand at 3021.926 kb on - strand at 3022.041 kb on + strand at 3022.041 kb on + strand at 3022.042 kb on - strand at 3022.042 kb on - strand at 3022.344 kb on - strand, within RPSI07_RS21675 at 3022.368 kb on - strand, within RPSI07_RS21675 at 3022.368 kb on - strand, within RPSI07_RS21675 at 3022.368 kb on - strand, within RPSI07_RS21675 at 3022.421 kb on + strand, within RPSI07_RS21675 at 3022.421 kb on + strand, within RPSI07_RS21675 at 3022.421 kb on + strand, within RPSI07_RS21675 at 3022.421 kb on + strand, within RPSI07_RS21675 at 3022.487 kb on + strand, within RPSI07_RS21675 at 3022.487 kb on + strand, within RPSI07_RS21675 at 3022.487 kb on + strand, within RPSI07_RS21675 at 3022.488 kb on - strand, within RPSI07_RS21675 at 3022.488 kb on - strand, within RPSI07_RS21675 at 3022.488 kb on - strand, within RPSI07_RS21675 at 3022.488 kb on - strand, within RPSI07_RS21675 at 3023.024 kb on + strand, within RPSI07_RS21680 at 3023.055 kb on + strand, within RPSI07_RS21680 at 3023.282 kb on + strand, within RPSI07_RS21680 at 3023.282 kb on + strand, within RPSI07_RS21680 at 3023.282 kb on + strand, within RPSI07_RS21680 at 3023.282 kb on + strand, within RPSI07_RS21680 at 3023.283 kb on - strand, within RPSI07_RS21680 at 3023.283 kb on - strand, within RPSI07_RS21680
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 3,021,159 + RPSI07_RS21670 0.53 -0.7 3,021,288 + RPSI07_RS21670 0.60 +0.0 3,021,307 - RPSI07_RS21670 0.61 -0.6 3,021,307 - RPSI07_RS21670 0.61 +0.9 3,021,429 + RPSI07_RS21670 0.68 -0.5 3,021,430 - RPSI07_RS21670 0.68 -1.2 3,021,430 - RPSI07_RS21670 0.68 -1.2 3,021,430 - RPSI07_RS21670 0.68 +0.6 3,021,430 - RPSI07_RS21670 0.68 -1.2 3,021,430 - RPSI07_RS21670 0.68 +0.2 3,021,430 - RPSI07_RS21670 0.68 -0.2 3,021,474 + RPSI07_RS21670 0.70 +0.4 3,021,474 + RPSI07_RS21670 0.70 +1.8 3,021,474 + RPSI07_RS21670 0.70 -0.2 3,021,474 + RPSI07_RS21670 0.70 -0.4 3,021,475 - RPSI07_RS21670 0.71 +2.1 3,021,475 - RPSI07_RS21670 0.71 +0.4 3,021,489 + RPSI07_RS21670 0.71 +0.3 3,021,489 + RPSI07_RS21670 0.71 +0.8 3,021,489 + RPSI07_RS21670 0.71 -1.6 3,021,489 + RPSI07_RS21670 0.71 -0.3 3,021,490 - RPSI07_RS21670 0.71 +1.2 3,021,490 - RPSI07_RS21670 0.71 +0.0 3,021,490 - RPSI07_RS21670 0.71 +0.2 3,021,490 - RPSI07_RS21670 0.71 +0.2 3,021,534 + RPSI07_RS21670 0.74 +0.1 3,021,544 - RPSI07_RS21670 0.74 +2.2 3,021,912 + -0.0 3,021,912 + -0.1 3,021,913 - +0.8 3,021,913 - -0.1 3,021,926 - +0.4 3,022,041 + +0.1 3,022,041 + +0.9 3,022,042 - +0.5 3,022,042 - +0.8 3,022,344 - RPSI07_RS21675 0.54 -1.1 3,022,368 - RPSI07_RS21675 0.60 +0.4 3,022,368 - RPSI07_RS21675 0.60 +0.3 3,022,368 - RPSI07_RS21675 0.60 +1.8 3,022,421 + RPSI07_RS21675 0.72 -1.5 3,022,421 + RPSI07_RS21675 0.72 +0.1 3,022,421 + RPSI07_RS21675 0.72 -0.4 3,022,421 + RPSI07_RS21675 0.72 -0.4 3,022,487 + RPSI07_RS21675 0.88 -0.1 3,022,487 + RPSI07_RS21675 0.88 -0.5 3,022,487 + RPSI07_RS21675 0.88 -0.3 3,022,488 - RPSI07_RS21675 0.88 +1.6 3,022,488 - RPSI07_RS21675 0.88 -0.2 3,022,488 - RPSI07_RS21675 0.88 -0.4 3,022,488 - RPSI07_RS21675 0.88 -0.8 3,023,024 + RPSI07_RS21680 0.55 +0.1 3,023,055 + RPSI07_RS21680 0.59 -0.1 3,023,282 + RPSI07_RS21680 0.88 +0.1 3,023,282 + RPSI07_RS21680 0.88 +2.4 3,023,282 + RPSI07_RS21680 0.88 -0.1 3,023,282 + RPSI07_RS21680 0.88 +0.8 3,023,283 - RPSI07_RS21680 0.88 -0.1 3,023,283 - RPSI07_RS21680 0.88 -0.6
Or see this region's nucleotide sequence