Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS12080 and RPSI07_RS12085 are separated by 343 nucleotides RPSI07_RS12085 and RPSI07_RS12090 are separated by 226 nucleotides RPSI07_RS12090 and RPSI07_RS12095 are separated by 3 nucleotides
RPSI07_RS12080: RPSI07_RS12080 - DUF3313 domain-containing protein, at 913,451 to 914,113
_RS12080
RPSI07_RS12085: RPSI07_RS12085 - TetR/AcrR family transcriptional regulator, at 914,457 to 915,086
_RS12085
RPSI07_RS12090: RPSI07_RS12090 - membrane protein, at 915,313 to 915,969
_RS12090
RPSI07_RS12095: RPSI07_RS12095 - transglutaminase, at 915,973 to 916,818
_RS12095
Position (kb) 914
915
916 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 913.566 kb on + strand, within RPSI07_RS12080 at 913.566 kb on + strand, within RPSI07_RS12080 at 913.567 kb on - strand, within RPSI07_RS12080 at 913.600 kb on - strand, within RPSI07_RS12080 at 913.614 kb on + strand, within RPSI07_RS12080 at 913.615 kb on - strand, within RPSI07_RS12080 at 913.671 kb on + strand, within RPSI07_RS12080 at 913.671 kb on + strand, within RPSI07_RS12080 at 913.671 kb on + strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.672 kb on - strand, within RPSI07_RS12080 at 913.830 kb on + strand, within RPSI07_RS12080 at 913.830 kb on + strand, within RPSI07_RS12080 at 913.830 kb on + strand, within RPSI07_RS12080 at 913.830 kb on + strand, within RPSI07_RS12080 at 913.831 kb on - strand at 913.893 kb on + strand, within RPSI07_RS12080 at 913.926 kb on + strand, within RPSI07_RS12080 at 913.953 kb on + strand, within RPSI07_RS12080 at 913.953 kb on + strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.954 kb on - strand, within RPSI07_RS12080 at 913.959 kb on + strand, within RPSI07_RS12080 at 914.001 kb on + strand, within RPSI07_RS12080 at 914.001 kb on + strand, within RPSI07_RS12080 at 914.001 kb on + strand, within RPSI07_RS12080 at 914.001 kb on + strand, within RPSI07_RS12080 at 914.002 kb on - strand, within RPSI07_RS12080 at 914.002 kb on - strand, within RPSI07_RS12080 at 914.002 kb on - strand, within RPSI07_RS12080 at 914.196 kb on + strand at 914.196 kb on + strand at 914.196 kb on + strand at 914.196 kb on + strand at 914.197 kb on - strand at 914.655 kb on + strand, within RPSI07_RS12085 at 914.655 kb on + strand, within RPSI07_RS12085 at 914.655 kb on + strand, within RPSI07_RS12085 at 914.656 kb on - strand, within RPSI07_RS12085 at 914.656 kb on - strand, within RPSI07_RS12085 at 914.656 kb on - strand, within RPSI07_RS12085 at 914.656 kb on - strand, within RPSI07_RS12085 at 914.720 kb on + strand, within RPSI07_RS12085 at 914.720 kb on + strand, within RPSI07_RS12085 at 914.721 kb on - strand, within RPSI07_RS12085 at 914.783 kb on + strand, within RPSI07_RS12085 at 914.783 kb on + strand, within RPSI07_RS12085 at 914.783 kb on + strand, within RPSI07_RS12085 at 914.784 kb on - strand, within RPSI07_RS12085 at 914.784 kb on - strand, within RPSI07_RS12085 at 915.198 kb on - strand at 915.216 kb on - strand at 915.268 kb on + strand at 915.268 kb on + strand at 915.268 kb on + strand at 915.268 kb on + strand at 915.268 kb on + strand at 915.268 kb on + strand at 915.269 kb on - strand at 915.386 kb on - strand, within RPSI07_RS12090 at 915.386 kb on - strand, within RPSI07_RS12090 at 915.401 kb on - strand, within RPSI07_RS12090 at 915.443 kb on + strand, within RPSI07_RS12090 at 915.473 kb on + strand, within RPSI07_RS12090 at 915.474 kb on - strand, within RPSI07_RS12090 at 915.474 kb on - strand, within RPSI07_RS12090 at 915.474 kb on - strand, within RPSI07_RS12090 at 915.474 kb on - strand, within RPSI07_RS12090 at 915.564 kb on + strand, within RPSI07_RS12090 at 915.565 kb on - strand, within RPSI07_RS12090 at 915.574 kb on + strand, within RPSI07_RS12090 at 915.574 kb on + strand, within RPSI07_RS12090 at 915.575 kb on - strand, within RPSI07_RS12090 at 915.619 kb on - strand, within RPSI07_RS12090 at 915.664 kb on + strand, within RPSI07_RS12090 at 915.665 kb on - strand, within RPSI07_RS12090 at 915.665 kb on - strand, within RPSI07_RS12090 at 915.665 kb on - strand, within RPSI07_RS12090 at 916.061 kb on + strand, within RPSI07_RS12095 at 916.061 kb on + strand, within RPSI07_RS12095
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 913,566 + RPSI07_RS12080 0.17 +0.2 913,566 + RPSI07_RS12080 0.17 -0.2 913,567 - RPSI07_RS12080 0.17 +0.5 913,600 - RPSI07_RS12080 0.22 +0.8 913,614 + RPSI07_RS12080 0.25 -0.2 913,615 - RPSI07_RS12080 0.25 +0.5 913,671 + RPSI07_RS12080 0.33 +1.2 913,671 + RPSI07_RS12080 0.33 -1.6 913,671 + RPSI07_RS12080 0.33 -0.2 913,672 - RPSI07_RS12080 0.33 -0.7 913,672 - RPSI07_RS12080 0.33 -0.1 913,672 - RPSI07_RS12080 0.33 -0.0 913,672 - RPSI07_RS12080 0.33 +0.5 913,672 - RPSI07_RS12080 0.33 -0.0 913,672 - RPSI07_RS12080 0.33 +0.6 913,672 - RPSI07_RS12080 0.33 +0.8 913,672 - RPSI07_RS12080 0.33 -0.2 913,672 - RPSI07_RS12080 0.33 +0.8 913,672 - RPSI07_RS12080 0.33 -0.1 913,830 + RPSI07_RS12080 0.57 -0.2 913,830 + RPSI07_RS12080 0.57 -0.2 913,830 + RPSI07_RS12080 0.57 +0.2 913,830 + RPSI07_RS12080 0.57 +0.2 913,831 - +0.5 913,893 + RPSI07_RS12080 0.67 -0.2 913,926 + RPSI07_RS12080 0.72 +0.8 913,953 + RPSI07_RS12080 0.76 -0.3 913,953 + RPSI07_RS12080 0.76 -0.2 913,954 - RPSI07_RS12080 0.76 -1.2 913,954 - RPSI07_RS12080 0.76 +0.1 913,954 - RPSI07_RS12080 0.76 +0.3 913,954 - RPSI07_RS12080 0.76 -0.5 913,954 - RPSI07_RS12080 0.76 +0.8 913,954 - RPSI07_RS12080 0.76 +0.2 913,954 - RPSI07_RS12080 0.76 +0.6 913,954 - RPSI07_RS12080 0.76 +0.0 913,959 + RPSI07_RS12080 0.77 -0.6 914,001 + RPSI07_RS12080 0.83 +0.8 914,001 + RPSI07_RS12080 0.83 -1.0 914,001 + RPSI07_RS12080 0.83 -0.5 914,001 + RPSI07_RS12080 0.83 -0.4 914,002 - RPSI07_RS12080 0.83 +0.8 914,002 - RPSI07_RS12080 0.83 -1.3 914,002 - RPSI07_RS12080 0.83 +0.1 914,196 + +0.1 914,196 + -0.2 914,196 + +0.6 914,196 + -0.5 914,197 - -0.2 914,655 + RPSI07_RS12085 0.31 -3.0 914,655 + RPSI07_RS12085 0.31 -3.7 914,655 + RPSI07_RS12085 0.31 -1.6 914,656 - RPSI07_RS12085 0.32 -1.8 914,656 - RPSI07_RS12085 0.32 -1.3 914,656 - RPSI07_RS12085 0.32 -2.2 914,656 - RPSI07_RS12085 0.32 -1.9 914,720 + RPSI07_RS12085 0.42 -1.9 914,720 + RPSI07_RS12085 0.42 -2.7 914,721 - RPSI07_RS12085 0.42 -2.8 914,783 + RPSI07_RS12085 0.52 -2.9 914,783 + RPSI07_RS12085 0.52 -4.4 914,783 + RPSI07_RS12085 0.52 -3.3 914,784 - RPSI07_RS12085 0.52 -1.1 914,784 - RPSI07_RS12085 0.52 -0.8 915,198 - -0.3 915,216 - -0.7 915,268 + +2.9 915,268 + -1.2 915,268 + -1.5 915,268 + -0.7 915,268 + -1.2 915,268 + -0.3 915,269 - -0.2 915,386 - RPSI07_RS12090 0.11 -1.4 915,386 - RPSI07_RS12090 0.11 -1.5 915,401 - RPSI07_RS12090 0.13 -0.8 915,443 + RPSI07_RS12090 0.20 +0.2 915,473 + RPSI07_RS12090 0.24 -1.2 915,474 - RPSI07_RS12090 0.25 -1.7 915,474 - RPSI07_RS12090 0.25 +0.8 915,474 - RPSI07_RS12090 0.25 -0.2 915,474 - RPSI07_RS12090 0.25 -0.8 915,564 + RPSI07_RS12090 0.38 -1.8 915,565 - RPSI07_RS12090 0.38 -1.2 915,574 + RPSI07_RS12090 0.40 -1.0 915,574 + RPSI07_RS12090 0.40 -1.8 915,575 - RPSI07_RS12090 0.40 -1.2 915,619 - RPSI07_RS12090 0.47 -1.6 915,664 + RPSI07_RS12090 0.53 -0.8 915,665 - RPSI07_RS12090 0.54 -1.6 915,665 - RPSI07_RS12090 0.54 -1.6 915,665 - RPSI07_RS12090 0.54 +0.2 916,061 + RPSI07_RS12095 0.10 -0.4 916,061 + RPSI07_RS12095 0.10 +0.8
Or see this region's nucleotide sequence