Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS01350 and RPSI07_RS24195 are separated by 126 nucleotides RPSI07_RS24195 and RPSI07_RS01355 are separated by 321 nucleotides RPSI07_RS01355 and RPSI07_RS01360 are separated by 94 nucleotides
RPSI07_RS01350: RPSI07_RS01350 - GGDEF domain-containing protein, at 370,503 to 373,190
_RS01350
RPSI07_RS24195: RPSI07_RS24195 - hypothetical protein, at 373,317 to 373,685
_RS24195
RPSI07_RS01355: RPSI07_RS01355 - DNA-binding protein, at 374,007 to 374,297
_RS01355
RPSI07_RS01360: RPSI07_RS01360 - GntR family transcriptional regulator, at 374,392 to 375,120
_RS01360
Position (kb) 373
374 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 372.677 kb on - strand, within RPSI07_RS01350 at 373.108 kb on + strand at 373.108 kb on + strand at 373.108 kb on + strand at 373.108 kb on + strand at 373.108 kb on + strand at 373.109 kb on - strand at 373.109 kb on - strand at 373.109 kb on - strand at 373.109 kb on - strand at 373.135 kb on + strand at 373.135 kb on + strand at 373.135 kb on + strand at 373.135 kb on + strand at 373.135 kb on + strand at 373.135 kb on + strand at 373.135 kb on + strand at 373.135 kb on + strand at 373.135 kb on + strand at 373.136 kb on - strand at 373.136 kb on - strand at 373.136 kb on - strand at 373.136 kb on - strand at 373.136 kb on - strand at 373.136 kb on - strand at 373.136 kb on - strand at 373.136 kb on - strand at 373.171 kb on + strand at 373.216 kb on + strand at 373.216 kb on + strand at 373.217 kb on - strand at 373.281 kb on - strand at 373.549 kb on + strand, within RPSI07_RS24195 at 373.549 kb on + strand, within RPSI07_RS24195 at 373.591 kb on + strand, within RPSI07_RS24195 at 373.591 kb on + strand, within RPSI07_RS24195 at 373.591 kb on + strand, within RPSI07_RS24195 at 373.591 kb on + strand, within RPSI07_RS24195 at 373.713 kb on + strand at 373.715 kb on + strand at 373.715 kb on + strand at 373.715 kb on + strand at 373.715 kb on + strand at 373.715 kb on + strand at 373.715 kb on + strand at 373.715 kb on + strand at 373.716 kb on - strand at 373.733 kb on + strand at 373.733 kb on + strand at 373.733 kb on + strand at 373.758 kb on + strand at 373.758 kb on + strand at 373.758 kb on + strand at 374.006 kb on + strand at 374.016 kb on + strand at 374.017 kb on - strand at 374.124 kb on + strand, within RPSI07_RS01355 at 374.124 kb on + strand, within RPSI07_RS01355 at 374.125 kb on - strand, within RPSI07_RS01355 at 374.205 kb on + strand, within RPSI07_RS01355 at 374.205 kb on + strand, within RPSI07_RS01355 at 374.205 kb on + strand, within RPSI07_RS01355 at 374.205 kb on + strand, within RPSI07_RS01355 at 374.205 kb on + strand, within RPSI07_RS01355 at 374.205 kb on + strand, within RPSI07_RS01355 at 374.206 kb on - strand, within RPSI07_RS01355 at 374.206 kb on - strand, within RPSI07_RS01355 at 374.380 kb on + strand at 374.380 kb on + strand at 374.380 kb on + strand at 374.380 kb on + strand at 374.380 kb on + strand at 374.380 kb on + strand at 374.381 kb on - strand at 374.381 kb on - strand at 374.381 kb on - strand at 374.381 kb on - strand at 374.381 kb on - strand at 374.381 kb on - strand at 374.382 kb on + strand at 374.382 kb on + strand at 374.383 kb on - strand at 374.383 kb on - strand at 374.383 kb on - strand at 374.413 kb on + strand at 374.413 kb on + strand at 374.413 kb on + strand at 374.414 kb on - strand at 374.414 kb on - strand at 374.635 kb on + strand, within RPSI07_RS01360 at 374.635 kb on + strand, within RPSI07_RS01360 at 374.668 kb on + strand, within RPSI07_RS01360 at 374.669 kb on - strand, within RPSI07_RS01360 at 374.669 kb on - strand, within RPSI07_RS01360 at 374.669 kb on - strand, within RPSI07_RS01360 at 374.669 kb on - strand, within RPSI07_RS01360 at 374.669 kb on - strand, within RPSI07_RS01360
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 372,677 - RPSI07_RS01350 0.81 -0.2 373,108 + -0.2 373,108 + +0.6 373,108 + -1.5 373,108 + +0.3 373,108 + +2.1 373,109 - +0.3 373,109 - +0.1 373,109 - -0.5 373,109 - -1.2 373,135 + +0.3 373,135 + -0.7 373,135 + -0.1 373,135 + +0.2 373,135 + -0.5 373,135 + -0.0 373,135 + +0.5 373,135 + -0.2 373,135 + -0.0 373,136 - -0.3 373,136 - -2.5 373,136 - -0.5 373,136 - +0.0 373,136 - -0.5 373,136 - -0.7 373,136 - +0.2 373,136 - -0.5 373,171 + +0.1 373,216 + +0.4 373,216 + +0.4 373,217 - -1.0 373,281 - -0.7 373,549 + RPSI07_RS24195 0.63 +0.9 373,549 + RPSI07_RS24195 0.63 +0.3 373,591 + RPSI07_RS24195 0.74 -0.2 373,591 + RPSI07_RS24195 0.74 -0.1 373,591 + RPSI07_RS24195 0.74 +0.3 373,591 + RPSI07_RS24195 0.74 +0.7 373,713 + +0.2 373,715 + -0.2 373,715 + +1.2 373,715 + +0.6 373,715 + -0.1 373,715 + +0.8 373,715 + +0.7 373,715 + +3.1 373,716 - +1.5 373,733 + +0.6 373,733 + -0.2 373,733 + +0.6 373,758 + +0.2 373,758 + -0.1 373,758 + +0.6 374,006 + +0.2 374,016 + -0.2 374,017 - -0.6 374,124 + RPSI07_RS01355 0.40 +0.8 374,124 + RPSI07_RS01355 0.40 -1.2 374,125 - RPSI07_RS01355 0.41 -0.8 374,205 + RPSI07_RS01355 0.68 -0.4 374,205 + RPSI07_RS01355 0.68 -0.2 374,205 + RPSI07_RS01355 0.68 -0.2 374,205 + RPSI07_RS01355 0.68 +2.4 374,205 + RPSI07_RS01355 0.68 -0.2 374,205 + RPSI07_RS01355 0.68 +0.8 374,206 - RPSI07_RS01355 0.68 -1.8 374,206 - RPSI07_RS01355 0.68 +0.2 374,380 + -1.5 374,380 + +1.1 374,380 + -1.4 374,380 + -0.2 374,380 + +1.6 374,380 + -1.4 374,381 - -0.5 374,381 - +0.5 374,381 - -0.2 374,381 - -0.0 374,381 - +0.8 374,381 - +0.0 374,382 + -0.6 374,382 + -0.8 374,383 - +1.5 374,383 - -0.1 374,383 - +1.2 374,413 + +0.8 374,413 + +0.8 374,413 + -0.4 374,414 - +0.4 374,414 - -0.4 374,635 + RPSI07_RS01360 0.33 -0.1 374,635 + RPSI07_RS01360 0.33 -1.0 374,668 + RPSI07_RS01360 0.38 -0.3 374,669 - RPSI07_RS01360 0.38 -0.3 374,669 - RPSI07_RS01360 0.38 -0.6 374,669 - RPSI07_RS01360 0.38 -0.3 374,669 - RPSI07_RS01360 0.38 -0.4 374,669 - RPSI07_RS01360 0.38 -0.2
Or see this region's nucleotide sequence