Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS22120 and RPSI07_RS22125 are separated by 33 nucleotides RPSI07_RS22125 and RPSI07_RS22130 are separated by 97 nucleotides RPSI07_RS22130 and RPSI07_RS22135 are separated by 51 nucleotides RPSI07_RS22135 and RPSI07_RS22140 are separated by 347 nucleotides
RPSI07_RS22120: RPSI07_RS22120 - lipopolysaccharide transport periplasmic protein LptA, at 3,132,685 to 3,133,281
_RS22120
RPSI07_RS22125: RPSI07_RS22125 - LPS export ABC transporter ATP-binding protein, at 3,133,315 to 3,134,103
_RS22125
RPSI07_RS22130: RPSI07_RS22130 - RNA polymerase factor sigma-54, at 3,134,201 to 3,135,700
_RS22130
RPSI07_RS22135: RPSI07_RS22135 - ribosomal subunit interface protein, at 3,135,752 to 3,136,105
_RS22135
RPSI07_RS22140: RPSI07_RS22140 - PTS IIA-like nitrogen-regulatory protein PtsN, at 3,136,453 to 3,136,908
_RS22140
Position (kb)
3134
3135
3136 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3134.101 kb on + strand at 3134.120 kb on + strand at 3134.127 kb on + strand at 3134.127 kb on + strand at 3134.127 kb on + strand at 3134.127 kb on + strand at 3134.127 kb on + strand at 3134.135 kb on + strand at 3134.135 kb on + strand at 3134.188 kb on + strand at 3134.188 kb on + strand at 3134.189 kb on - strand at 3134.189 kb on - strand at 3134.189 kb on - strand at 3134.189 kb on - strand at 3134.194 kb on - strand at 3134.495 kb on + strand, within RPSI07_RS22130 at 3134.495 kb on + strand, within RPSI07_RS22130 at 3134.729 kb on + strand, within RPSI07_RS22130 at 3134.730 kb on - strand, within RPSI07_RS22130 at 3134.963 kb on + strand, within RPSI07_RS22130 at 3134.963 kb on + strand, within RPSI07_RS22130 at 3134.964 kb on - strand, within RPSI07_RS22130 at 3135.107 kb on - strand, within RPSI07_RS22130 at 3135.177 kb on - strand, within RPSI07_RS22130 at 3135.379 kb on - strand, within RPSI07_RS22130 at 3135.385 kb on + strand, within RPSI07_RS22130 at 3135.397 kb on - strand, within RPSI07_RS22130 at 3135.434 kb on + strand, within RPSI07_RS22130 at 3135.434 kb on + strand, within RPSI07_RS22130 at 3135.434 kb on + strand, within RPSI07_RS22130 at 3135.434 kb on + strand, within RPSI07_RS22130 at 3135.434 kb on + strand, within RPSI07_RS22130 at 3135.435 kb on - strand, within RPSI07_RS22130 at 3135.471 kb on - strand, within RPSI07_RS22130 at 3135.489 kb on + strand, within RPSI07_RS22130 at 3135.489 kb on + strand, within RPSI07_RS22130 at 3135.489 kb on + strand, within RPSI07_RS22130 at 3135.490 kb on - strand, within RPSI07_RS22130 at 3135.490 kb on - strand, within RPSI07_RS22130 at 3135.490 kb on - strand, within RPSI07_RS22130 at 3135.528 kb on + strand, within RPSI07_RS22130 at 3135.528 kb on + strand, within RPSI07_RS22130 at 3135.553 kb on - strand at 3135.607 kb on - strand at 3135.696 kb on + strand at 3135.699 kb on - strand at 3135.752 kb on - strand at 3135.807 kb on - strand, within RPSI07_RS22135 at 3135.873 kb on + strand, within RPSI07_RS22135 at 3135.884 kb on - strand, within RPSI07_RS22135 at 3135.977 kb on + strand, within RPSI07_RS22135 at 3135.978 kb on - strand, within RPSI07_RS22135 at 3136.028 kb on + strand, within RPSI07_RS22135 at 3136.028 kb on + strand, within RPSI07_RS22135 at 3136.029 kb on - strand, within RPSI07_RS22135 at 3136.029 kb on - strand, within RPSI07_RS22135 at 3136.103 kb on + strand at 3136.104 kb on - strand at 3136.104 kb on - strand at 3136.104 kb on - strand at 3136.194 kb on + strand at 3136.205 kb on - strand at 3136.228 kb on + strand at 3136.228 kb on + strand at 3136.228 kb on + strand at 3136.228 kb on + strand at 3136.229 kb on - strand at 3136.386 kb on + strand at 3136.386 kb on + strand at 3136.386 kb on + strand at 3136.386 kb on + strand at 3136.386 kb on + strand at 3136.386 kb on + strand at 3136.386 kb on + strand at 3136.387 kb on - strand at 3136.387 kb on - strand at 3136.387 kb on - strand at 3136.387 kb on - strand at 3136.388 kb on + strand at 3136.388 kb on + strand at 3136.388 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 3,134,101 + +0.5 3,134,120 + +0.5 3,134,127 + -0.6 3,134,127 + -0.1 3,134,127 + +0.2 3,134,127 + -0.5 3,134,127 + +0.8 3,134,135 + +1.0 3,134,135 + +1.0 3,134,188 + -0.4 3,134,188 + +0.6 3,134,189 - -0.1 3,134,189 - +1.0 3,134,189 - +1.6 3,134,189 - +0.1 3,134,194 - +0.4 3,134,495 + RPSI07_RS22130 0.20 +0.1 3,134,495 + RPSI07_RS22130 0.20 -0.3 3,134,729 + RPSI07_RS22130 0.35 -0.8 3,134,730 - RPSI07_RS22130 0.35 -0.1 3,134,963 + RPSI07_RS22130 0.51 +0.9 3,134,963 + RPSI07_RS22130 0.51 +1.0 3,134,964 - RPSI07_RS22130 0.51 -0.0 3,135,107 - RPSI07_RS22130 0.60 +1.0 3,135,177 - RPSI07_RS22130 0.65 -0.3 3,135,379 - RPSI07_RS22130 0.79 -0.3 3,135,385 + RPSI07_RS22130 0.79 -0.2 3,135,397 - RPSI07_RS22130 0.80 -0.6 3,135,434 + RPSI07_RS22130 0.82 -0.8 3,135,434 + RPSI07_RS22130 0.82 -0.3 3,135,434 + RPSI07_RS22130 0.82 -0.1 3,135,434 + RPSI07_RS22130 0.82 -0.2 3,135,434 + RPSI07_RS22130 0.82 +1.3 3,135,435 - RPSI07_RS22130 0.82 +1.0 3,135,471 - RPSI07_RS22130 0.85 +0.8 3,135,489 + RPSI07_RS22130 0.86 -0.8 3,135,489 + RPSI07_RS22130 0.86 +0.5 3,135,489 + RPSI07_RS22130 0.86 -0.3 3,135,490 - RPSI07_RS22130 0.86 +1.0 3,135,490 - RPSI07_RS22130 0.86 +0.0 3,135,490 - RPSI07_RS22130 0.86 -0.3 3,135,528 + RPSI07_RS22130 0.88 +0.7 3,135,528 + RPSI07_RS22130 0.88 +0.1 3,135,553 - +0.6 3,135,607 - -1.0 3,135,696 + +0.4 3,135,699 - -1.8 3,135,752 - -0.4 3,135,807 - RPSI07_RS22135 0.16 +1.0 3,135,873 + RPSI07_RS22135 0.34 +1.0 3,135,884 - RPSI07_RS22135 0.37 +1.3 3,135,977 + RPSI07_RS22135 0.64 +0.4 3,135,978 - RPSI07_RS22135 0.64 +1.0 3,136,028 + RPSI07_RS22135 0.78 +0.1 3,136,028 + RPSI07_RS22135 0.78 -1.0 3,136,029 - RPSI07_RS22135 0.78 -1.0 3,136,029 - RPSI07_RS22135 0.78 +0.0 3,136,103 + -0.9 3,136,104 - -0.7 3,136,104 - +0.0 3,136,104 - -1.6 3,136,194 + +1.4 3,136,205 - -0.3 3,136,228 + -1.3 3,136,228 + +1.0 3,136,228 + +0.7 3,136,228 + -0.1 3,136,229 - +0.7 3,136,386 + +1.0 3,136,386 + +0.9 3,136,386 + +0.8 3,136,386 + +3.8 3,136,386 + +0.6 3,136,386 + +1.2 3,136,386 + +0.9 3,136,387 - -0.4 3,136,387 - -0.3 3,136,387 - +1.0 3,136,387 - -1.2 3,136,388 + +0.5 3,136,388 + +0.7 3,136,388 + +0.3
Or see this region's nucleotide sequence