Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS22130

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS22120 and RPSI07_RS22125 are separated by 33 nucleotidesRPSI07_RS22125 and RPSI07_RS22130 are separated by 97 nucleotidesRPSI07_RS22130 and RPSI07_RS22135 are separated by 51 nucleotidesRPSI07_RS22135 and RPSI07_RS22140 are separated by 347 nucleotides RPSI07_RS22120: RPSI07_RS22120 - lipopolysaccharide transport periplasmic protein LptA, at 3,132,685 to 3,133,281 _RS22120 RPSI07_RS22125: RPSI07_RS22125 - LPS export ABC transporter ATP-binding protein, at 3,133,315 to 3,134,103 _RS22125 RPSI07_RS22130: RPSI07_RS22130 - RNA polymerase factor sigma-54, at 3,134,201 to 3,135,700 _RS22130 RPSI07_RS22135: RPSI07_RS22135 - ribosomal subunit interface protein, at 3,135,752 to 3,136,105 _RS22135 RPSI07_RS22140: RPSI07_RS22140 - PTS IIA-like nitrogen-regulatory protein PtsN, at 3,136,453 to 3,136,908 _RS22140 Position (kb) 3134 3135 3136Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3134.101 kb on + strandat 3134.120 kb on + strandat 3134.127 kb on + strandat 3134.127 kb on + strandat 3134.127 kb on + strandat 3134.127 kb on + strandat 3134.127 kb on + strandat 3134.135 kb on + strandat 3134.135 kb on + strandat 3134.188 kb on + strandat 3134.188 kb on + strandat 3134.189 kb on - strandat 3134.189 kb on - strandat 3134.189 kb on - strandat 3134.189 kb on - strandat 3134.194 kb on - strandat 3134.495 kb on + strand, within RPSI07_RS22130at 3134.495 kb on + strand, within RPSI07_RS22130at 3134.729 kb on + strand, within RPSI07_RS22130at 3134.730 kb on - strand, within RPSI07_RS22130at 3134.963 kb on + strand, within RPSI07_RS22130at 3134.963 kb on + strand, within RPSI07_RS22130at 3134.964 kb on - strand, within RPSI07_RS22130at 3135.107 kb on - strand, within RPSI07_RS22130at 3135.177 kb on - strand, within RPSI07_RS22130at 3135.379 kb on - strand, within RPSI07_RS22130at 3135.385 kb on + strand, within RPSI07_RS22130at 3135.397 kb on - strand, within RPSI07_RS22130at 3135.434 kb on + strand, within RPSI07_RS22130at 3135.434 kb on + strand, within RPSI07_RS22130at 3135.434 kb on + strand, within RPSI07_RS22130at 3135.434 kb on + strand, within RPSI07_RS22130at 3135.434 kb on + strand, within RPSI07_RS22130at 3135.435 kb on - strand, within RPSI07_RS22130at 3135.471 kb on - strand, within RPSI07_RS22130at 3135.489 kb on + strand, within RPSI07_RS22130at 3135.489 kb on + strand, within RPSI07_RS22130at 3135.489 kb on + strand, within RPSI07_RS22130at 3135.490 kb on - strand, within RPSI07_RS22130at 3135.490 kb on - strand, within RPSI07_RS22130at 3135.490 kb on - strand, within RPSI07_RS22130at 3135.528 kb on + strand, within RPSI07_RS22130at 3135.528 kb on + strand, within RPSI07_RS22130at 3135.553 kb on - strandat 3135.607 kb on - strandat 3135.696 kb on + strandat 3135.699 kb on - strandat 3135.752 kb on - strandat 3135.807 kb on - strand, within RPSI07_RS22135at 3135.873 kb on + strand, within RPSI07_RS22135at 3135.884 kb on - strand, within RPSI07_RS22135at 3135.977 kb on + strand, within RPSI07_RS22135at 3135.978 kb on - strand, within RPSI07_RS22135at 3136.028 kb on + strand, within RPSI07_RS22135at 3136.028 kb on + strand, within RPSI07_RS22135at 3136.029 kb on - strand, within RPSI07_RS22135at 3136.029 kb on - strand, within RPSI07_RS22135at 3136.103 kb on + strandat 3136.104 kb on - strandat 3136.104 kb on - strandat 3136.104 kb on - strandat 3136.194 kb on + strandat 3136.205 kb on - strandat 3136.228 kb on + strandat 3136.228 kb on + strandat 3136.228 kb on + strandat 3136.228 kb on + strandat 3136.229 kb on - strandat 3136.386 kb on + strandat 3136.386 kb on + strandat 3136.386 kb on + strandat 3136.386 kb on + strandat 3136.386 kb on + strandat 3136.386 kb on + strandat 3136.386 kb on + strandat 3136.387 kb on - strandat 3136.387 kb on - strandat 3136.387 kb on - strandat 3136.387 kb on - strandat 3136.388 kb on + strandat 3136.388 kb on + strandat 3136.388 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
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3,134,101 + +0.5
3,134,120 + +0.5
3,134,127 + -0.6
3,134,127 + -0.1
3,134,127 + +0.2
3,134,127 + -0.5
3,134,127 + +0.8
3,134,135 + +1.0
3,134,135 + +1.0
3,134,188 + -0.4
3,134,188 + +0.6
3,134,189 - -0.1
3,134,189 - +1.0
3,134,189 - +1.6
3,134,189 - +0.1
3,134,194 - +0.4
3,134,495 + RPSI07_RS22130 0.20 +0.1
3,134,495 + RPSI07_RS22130 0.20 -0.3
3,134,729 + RPSI07_RS22130 0.35 -0.8
3,134,730 - RPSI07_RS22130 0.35 -0.1
3,134,963 + RPSI07_RS22130 0.51 +0.9
3,134,963 + RPSI07_RS22130 0.51 +1.0
3,134,964 - RPSI07_RS22130 0.51 -0.0
3,135,107 - RPSI07_RS22130 0.60 +1.0
3,135,177 - RPSI07_RS22130 0.65 -0.3
3,135,379 - RPSI07_RS22130 0.79 -0.3
3,135,385 + RPSI07_RS22130 0.79 -0.2
3,135,397 - RPSI07_RS22130 0.80 -0.6
3,135,434 + RPSI07_RS22130 0.82 -0.8
3,135,434 + RPSI07_RS22130 0.82 -0.3
3,135,434 + RPSI07_RS22130 0.82 -0.1
3,135,434 + RPSI07_RS22130 0.82 -0.2
3,135,434 + RPSI07_RS22130 0.82 +1.3
3,135,435 - RPSI07_RS22130 0.82 +1.0
3,135,471 - RPSI07_RS22130 0.85 +0.8
3,135,489 + RPSI07_RS22130 0.86 -0.8
3,135,489 + RPSI07_RS22130 0.86 +0.5
3,135,489 + RPSI07_RS22130 0.86 -0.3
3,135,490 - RPSI07_RS22130 0.86 +1.0
3,135,490 - RPSI07_RS22130 0.86 +0.0
3,135,490 - RPSI07_RS22130 0.86 -0.3
3,135,528 + RPSI07_RS22130 0.88 +0.7
3,135,528 + RPSI07_RS22130 0.88 +0.1
3,135,553 - +0.6
3,135,607 - -1.0
3,135,696 + +0.4
3,135,699 - -1.8
3,135,752 - -0.4
3,135,807 - RPSI07_RS22135 0.16 +1.0
3,135,873 + RPSI07_RS22135 0.34 +1.0
3,135,884 - RPSI07_RS22135 0.37 +1.3
3,135,977 + RPSI07_RS22135 0.64 +0.4
3,135,978 - RPSI07_RS22135 0.64 +1.0
3,136,028 + RPSI07_RS22135 0.78 +0.1
3,136,028 + RPSI07_RS22135 0.78 -1.0
3,136,029 - RPSI07_RS22135 0.78 -1.0
3,136,029 - RPSI07_RS22135 0.78 +0.0
3,136,103 + -0.9
3,136,104 - -0.7
3,136,104 - +0.0
3,136,104 - -1.6
3,136,194 + +1.4
3,136,205 - -0.3
3,136,228 + -1.3
3,136,228 + +1.0
3,136,228 + +0.7
3,136,228 + -0.1
3,136,229 - +0.7
3,136,386 + +1.0
3,136,386 + +0.9
3,136,386 + +0.8
3,136,386 + +3.8
3,136,386 + +0.6
3,136,386 + +1.2
3,136,386 + +0.9
3,136,387 - -0.4
3,136,387 - -0.3
3,136,387 - +1.0
3,136,387 - -1.2
3,136,388 + +0.5
3,136,388 + +0.7
3,136,388 + +0.3

Or see this region's nucleotide sequence