Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS22425

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS22415 and RPSI07_RS22420 are separated by 72 nucleotidesRPSI07_RS22420 and RPSI07_RS22425 are separated by 67 nucleotidesRPSI07_RS22425 and RPSI07_RS22430 are separated by 139 nucleotidesRPSI07_RS22430 and RPSI07_RS22435 are separated by 210 nucleotides RPSI07_RS22415: RPSI07_RS22415 - S41 family peptidase, at 3,186,083 to 3,187,699 _RS22415 RPSI07_RS22420: RPSI07_RS22420 - molybdopterin-synthase adenylyltransferase MoeB, at 3,187,772 to 3,188,521 _RS22420 RPSI07_RS22425: RPSI07_RS22425 - bacterioferritin, at 3,188,589 to 3,189,068 _RS22425 RPSI07_RS22430: RPSI07_RS22430 - (2Fe-2S)-binding protein, at 3,189,208 to 3,189,483 _RS22430 RPSI07_RS22435: RPSI07_RS22435 - phosphoenolpyruvate--protein phosphotransferase, at 3,189,694 to 3,191,454 _RS22435 Position (kb) 3188 3189 3190Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3188.623 kb on + strandat 3188.624 kb on - strandat 3188.624 kb on - strandat 3188.728 kb on + strand, within RPSI07_RS22425at 3188.728 kb on + strand, within RPSI07_RS22425at 3188.728 kb on + strand, within RPSI07_RS22425at 3188.750 kb on - strand, within RPSI07_RS22425at 3188.750 kb on - strand, within RPSI07_RS22425at 3188.935 kb on + strand, within RPSI07_RS22425at 3188.935 kb on + strand, within RPSI07_RS22425at 3188.935 kb on + strand, within RPSI07_RS22425at 3188.935 kb on + strand, within RPSI07_RS22425at 3188.936 kb on - strand, within RPSI07_RS22425at 3188.936 kb on - strand, within RPSI07_RS22425at 3188.974 kb on + strand, within RPSI07_RS22425at 3188.974 kb on + strand, within RPSI07_RS22425at 3188.974 kb on + strand, within RPSI07_RS22425at 3188.975 kb on - strand, within RPSI07_RS22425at 3188.975 kb on - strand, within RPSI07_RS22425at 3188.975 kb on - strand, within RPSI07_RS22425at 3188.975 kb on - strand, within RPSI07_RS22425at 3188.975 kb on - strand, within RPSI07_RS22425at 3188.995 kb on + strand, within RPSI07_RS22425at 3188.995 kb on + strand, within RPSI07_RS22425at 3188.996 kb on - strand, within RPSI07_RS22425at 3188.996 kb on - strand, within RPSI07_RS22425at 3189.040 kb on + strandat 3189.056 kb on + strandat 3189.057 kb on - strandat 3189.274 kb on + strand, within RPSI07_RS22430at 3189.274 kb on + strand, within RPSI07_RS22430at 3189.275 kb on - strand, within RPSI07_RS22430at 3189.479 kb on + strandat 3189.479 kb on + strandat 3189.479 kb on + strandat 3189.479 kb on + strandat 3189.480 kb on - strandat 3189.480 kb on - strandat 3189.480 kb on - strandat 3189.480 kb on - strandat 3189.528 kb on - strandat 3189.990 kb on - strand, within RPSI07_RS22435at 3190.034 kb on + strand, within RPSI07_RS22435

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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3,188,623 + -0.0
3,188,624 - -0.2
3,188,624 - +1.2
3,188,728 + RPSI07_RS22425 0.29 -0.6
3,188,728 + RPSI07_RS22425 0.29 -0.1
3,188,728 + RPSI07_RS22425 0.29 -0.0
3,188,750 - RPSI07_RS22425 0.34 -0.7
3,188,750 - RPSI07_RS22425 0.34 -0.3
3,188,935 + RPSI07_RS22425 0.72 +1.2
3,188,935 + RPSI07_RS22425 0.72 -1.0
3,188,935 + RPSI07_RS22425 0.72 -0.4
3,188,935 + RPSI07_RS22425 0.72 -1.1
3,188,936 - RPSI07_RS22425 0.72 +0.2
3,188,936 - RPSI07_RS22425 0.72 +1.2
3,188,974 + RPSI07_RS22425 0.80 -0.4
3,188,974 + RPSI07_RS22425 0.80 -0.2
3,188,974 + RPSI07_RS22425 0.80 -0.3
3,188,975 - RPSI07_RS22425 0.80 +1.2
3,188,975 - RPSI07_RS22425 0.80 -0.1
3,188,975 - RPSI07_RS22425 0.80 +2.2
3,188,975 - RPSI07_RS22425 0.80 -0.4
3,188,975 - RPSI07_RS22425 0.80 -0.3
3,188,995 + RPSI07_RS22425 0.85 -0.4
3,188,995 + RPSI07_RS22425 0.85 -0.7
3,188,996 - RPSI07_RS22425 0.85 -0.3
3,188,996 - RPSI07_RS22425 0.85 +1.8
3,189,040 + +0.4
3,189,056 + +1.1
3,189,057 - +1.7
3,189,274 + RPSI07_RS22430 0.24 -0.4
3,189,274 + RPSI07_RS22430 0.24 -0.3
3,189,275 - RPSI07_RS22430 0.24 -0.4
3,189,479 + -1.4
3,189,479 + -1.1
3,189,479 + -1.3
3,189,479 + +0.2
3,189,480 - +0.2
3,189,480 - +0.5
3,189,480 - +0.3
3,189,480 - -1.6
3,189,528 - -1.5
3,189,990 - RPSI07_RS22435 0.17 +0.0
3,190,034 + RPSI07_RS22435 0.19 -4.5

Or see this region's nucleotide sequence