Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21790 and RPSI07_RS21795 are separated by 202 nucleotides RPSI07_RS21795 and RPSI07_RS21800 are separated by 66 nucleotides
RPSI07_RS21790: RPSI07_RS21790 - FAD-linked oxidase, at 3,048,285 to 3,052,310
_RS21790
RPSI07_RS21795: RPSI07_RS21795 - DedA family protein, at 3,052,513 to 3,053,094
_RS21795
RPSI07_RS21800: RPSI07_RS21800 - membrane protein, at 3,053,161 to 3,054,408
_RS21800
Position (kb)
3052
3053
3054 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3051.862 kb on + strand, within RPSI07_RS21790 at 3051.862 kb on + strand, within RPSI07_RS21790 at 3051.863 kb on - strand, within RPSI07_RS21790 at 3052.075 kb on + strand at 3052.075 kb on + strand at 3052.117 kb on + strand at 3052.118 kb on - strand at 3052.231 kb on + strand at 3052.231 kb on + strand at 3052.231 kb on + strand at 3052.231 kb on + strand at 3052.231 kb on + strand at 3052.231 kb on + strand at 3052.232 kb on - strand at 3052.232 kb on - strand at 3052.232 kb on - strand at 3052.235 kb on + strand at 3052.235 kb on + strand at 3052.235 kb on + strand at 3052.236 kb on - strand at 3052.237 kb on + strand at 3052.237 kb on + strand at 3052.237 kb on + strand at 3052.237 kb on + strand at 3052.238 kb on - strand at 3052.369 kb on - strand at 3052.419 kb on - strand at 3052.598 kb on + strand, within RPSI07_RS21795 at 3052.599 kb on - strand, within RPSI07_RS21795 at 3052.599 kb on - strand, within RPSI07_RS21795 at 3052.625 kb on + strand, within RPSI07_RS21795 at 3052.625 kb on + strand, within RPSI07_RS21795 at 3052.625 kb on + strand, within RPSI07_RS21795 at 3052.625 kb on + strand, within RPSI07_RS21795 at 3052.626 kb on - strand, within RPSI07_RS21795 at 3052.626 kb on - strand, within RPSI07_RS21795 at 3052.626 kb on - strand, within RPSI07_RS21795 at 3052.626 kb on - strand, within RPSI07_RS21795 at 3052.626 kb on - strand, within RPSI07_RS21795 at 3052.748 kb on + strand, within RPSI07_RS21795 at 3052.749 kb on - strand, within RPSI07_RS21795 at 3052.871 kb on + strand, within RPSI07_RS21795 at 3052.872 kb on - strand, within RPSI07_RS21795 at 3052.906 kb on - strand, within RPSI07_RS21795 at 3052.906 kb on - strand, within RPSI07_RS21795 at 3052.965 kb on - strand, within RPSI07_RS21795 at 3053.106 kb on + strand at 3053.107 kb on - strand at 3053.177 kb on + strand at 3053.177 kb on + strand at 3053.178 kb on - strand at 3053.241 kb on - strand at 3053.241 kb on - strand at 3053.241 kb on - strand at 3053.274 kb on + strand at 3053.274 kb on - strand at 3053.274 kb on - strand at 3053.289 kb on - strand, within RPSI07_RS21800 at 3053.289 kb on - strand, within RPSI07_RS21800 at 3053.289 kb on - strand, within RPSI07_RS21800 at 3053.289 kb on - strand, within RPSI07_RS21800 at 3053.354 kb on + strand, within RPSI07_RS21800 at 3053.354 kb on + strand, within RPSI07_RS21800 at 3053.550 kb on - strand, within RPSI07_RS21800 at 3053.550 kb on - strand, within RPSI07_RS21800 at 3053.609 kb on + strand, within RPSI07_RS21800 at 3053.610 kb on - strand, within RPSI07_RS21800 at 3053.610 kb on - strand, within RPSI07_RS21800 at 3053.610 kb on - strand, within RPSI07_RS21800 at 3053.610 kb on - strand, within RPSI07_RS21800 at 3053.771 kb on + strand, within RPSI07_RS21800 at 3053.772 kb on - strand, within RPSI07_RS21800 at 3053.772 kb on - strand, within RPSI07_RS21800
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 3,051,862 + RPSI07_RS21790 0.89 -1.0 3,051,862 + RPSI07_RS21790 0.89 -0.8 3,051,863 - RPSI07_RS21790 0.89 +1.2 3,052,075 + +0.9 3,052,075 + -0.2 3,052,117 + +0.2 3,052,118 - +2.5 3,052,231 + -0.5 3,052,231 + -0.5 3,052,231 + +0.5 3,052,231 + +0.8 3,052,231 + +0.0 3,052,231 + +0.2 3,052,232 - -0.2 3,052,232 - +0.0 3,052,232 - -0.5 3,052,235 + -0.1 3,052,235 + +0.3 3,052,235 + -0.4 3,052,236 - -0.0 3,052,237 + -0.4 3,052,237 + +0.7 3,052,237 + -0.4 3,052,237 + +1.8 3,052,238 - -0.0 3,052,369 - -0.1 3,052,419 - +0.4 3,052,598 + RPSI07_RS21795 0.15 +0.6 3,052,599 - RPSI07_RS21795 0.15 -1.0 3,052,599 - RPSI07_RS21795 0.15 -0.2 3,052,625 + RPSI07_RS21795 0.19 +0.3 3,052,625 + RPSI07_RS21795 0.19 +0.2 3,052,625 + RPSI07_RS21795 0.19 +0.4 3,052,625 + RPSI07_RS21795 0.19 -1.6 3,052,626 - RPSI07_RS21795 0.19 +0.2 3,052,626 - RPSI07_RS21795 0.19 -0.0 3,052,626 - RPSI07_RS21795 0.19 -1.3 3,052,626 - RPSI07_RS21795 0.19 -0.6 3,052,626 - RPSI07_RS21795 0.19 +0.2 3,052,748 + RPSI07_RS21795 0.40 -0.1 3,052,749 - RPSI07_RS21795 0.41 -0.5 3,052,871 + RPSI07_RS21795 0.62 +0.2 3,052,872 - RPSI07_RS21795 0.62 -1.2 3,052,906 - RPSI07_RS21795 0.68 -0.5 3,052,906 - RPSI07_RS21795 0.68 -0.4 3,052,965 - RPSI07_RS21795 0.78 +1.2 3,053,106 + +1.4 3,053,107 - -0.3 3,053,177 + -0.2 3,053,177 + +0.4 3,053,178 - -0.0 3,053,241 - -0.1 3,053,241 - +0.2 3,053,241 - +0.2 3,053,274 + -2.5 3,053,274 - -0.2 3,053,274 - -0.4 3,053,289 - RPSI07_RS21800 0.10 -0.6 3,053,289 - RPSI07_RS21800 0.10 -0.4 3,053,289 - RPSI07_RS21800 0.10 +1.2 3,053,289 - RPSI07_RS21800 0.10 +1.2 3,053,354 + RPSI07_RS21800 0.15 +0.9 3,053,354 + RPSI07_RS21800 0.15 -0.7 3,053,550 - RPSI07_RS21800 0.31 -0.3 3,053,550 - RPSI07_RS21800 0.31 -0.8 3,053,609 + RPSI07_RS21800 0.36 +0.6 3,053,610 - RPSI07_RS21800 0.36 -0.7 3,053,610 - RPSI07_RS21800 0.36 -0.8 3,053,610 - RPSI07_RS21800 0.36 -1.1 3,053,610 - RPSI07_RS21800 0.36 -1.2 3,053,771 + RPSI07_RS21800 0.49 -0.6 3,053,772 - RPSI07_RS21800 0.49 -0.6 3,053,772 - RPSI07_RS21800 0.49 -1.2
Or see this region's nucleotide sequence