Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21225 and RPSI07_RS21230 are separated by 53 nucleotides RPSI07_RS21230 and RPSI07_RS21235 are separated by 193 nucleotides
RPSI07_RS21225: RPSI07_RS21225 - hypothetical protein, at 2,926,954 to 2,927,591
_RS21225
RPSI07_RS21230: RPSI07_RS21230 - pilin, at 2,927,645 to 2,928,166
_RS21230
RPSI07_RS21235: RPSI07_RS21235 - hypothetical protein, at 2,928,360 to 2,929,058
_RS21235
Position (kb)
2927
2928
2929 Strain fitness (log2 ratio)
-2
-1
0
1 at 2926.726 kb on + strand at 2926.726 kb on + strand at 2926.727 kb on - strand at 2926.727 kb on - strand at 2926.727 kb on - strand at 2926.727 kb on - strand at 2926.727 kb on - strand at 2926.771 kb on + strand at 2926.771 kb on + strand at 2926.771 kb on + strand at 2926.772 kb on - strand at 2926.772 kb on - strand at 2926.772 kb on - strand at 2926.849 kb on + strand at 2926.849 kb on + strand at 2927.004 kb on + strand at 2927.005 kb on - strand at 2927.286 kb on + strand, within RPSI07_RS21225 at 2927.286 kb on + strand, within RPSI07_RS21225 at 2927.287 kb on - strand, within RPSI07_RS21225 at 2927.289 kb on + strand, within RPSI07_RS21225 at 2927.289 kb on + strand, within RPSI07_RS21225 at 2927.290 kb on - strand, within RPSI07_RS21225 at 2927.290 kb on - strand, within RPSI07_RS21225 at 2927.290 kb on - strand, within RPSI07_RS21225 at 2927.311 kb on - strand, within RPSI07_RS21225 at 2927.412 kb on + strand, within RPSI07_RS21225 at 2927.413 kb on - strand, within RPSI07_RS21225 at 2927.413 kb on - strand, within RPSI07_RS21225 at 2927.430 kb on + strand, within RPSI07_RS21225 at 2927.469 kb on + strand, within RPSI07_RS21225 at 2927.470 kb on - strand, within RPSI07_RS21225 at 2927.470 kb on - strand, within RPSI07_RS21225 at 2927.470 kb on - strand, within RPSI07_RS21225 at 2927.531 kb on + strand at 2927.627 kb on + strand at 2927.667 kb on + strand at 2927.668 kb on - strand at 2927.668 kb on - strand at 2927.668 kb on - strand at 2927.822 kb on + strand, within RPSI07_RS21230 at 2927.823 kb on - strand, within RPSI07_RS21230 at 2927.830 kb on + strand, within RPSI07_RS21230 at 2927.830 kb on + strand, within RPSI07_RS21230 at 2927.862 kb on + strand, within RPSI07_RS21230 at 2927.872 kb on + strand, within RPSI07_RS21230 at 2927.873 kb on - strand, within RPSI07_RS21230 at 2927.953 kb on + strand, within RPSI07_RS21230 at 2927.953 kb on + strand, within RPSI07_RS21230 at 2927.953 kb on + strand, within RPSI07_RS21230 at 2927.953 kb on + strand, within RPSI07_RS21230 at 2927.954 kb on - strand, within RPSI07_RS21230 at 2927.954 kb on - strand, within RPSI07_RS21230 at 2928.042 kb on + strand, within RPSI07_RS21230 at 2928.043 kb on - strand, within RPSI07_RS21230 at 2928.052 kb on - strand, within RPSI07_RS21230 at 2928.077 kb on + strand, within RPSI07_RS21230 at 2928.191 kb on + strand at 2928.280 kb on + strand at 2928.281 kb on - strand at 2928.514 kb on + strand, within RPSI07_RS21235 at 2928.515 kb on - strand, within RPSI07_RS21235 at 2928.811 kb on + strand, within RPSI07_RS21235 at 2928.811 kb on + strand, within RPSI07_RS21235 at 2928.845 kb on - strand, within RPSI07_RS21235 at 2929.113 kb on + strand at 2929.114 kb on - strand at 2929.115 kb on + strand at 2929.115 kb on + strand at 2929.115 kb on + strand at 2929.115 kb on + strand at 2929.115 kb on + strand at 2929.116 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 2,926,726 + -1.6 2,926,726 + +0.3 2,926,727 - -0.4 2,926,727 - +0.2 2,926,727 - -0.2 2,926,727 - -1.6 2,926,727 - -1.3 2,926,771 + +0.1 2,926,771 + -0.5 2,926,771 + +0.2 2,926,772 - -1.1 2,926,772 - +1.2 2,926,772 - +0.0 2,926,849 + -0.3 2,926,849 + -1.1 2,927,004 + +1.2 2,927,005 - -1.4 2,927,286 + RPSI07_RS21225 0.52 -0.7 2,927,286 + RPSI07_RS21225 0.52 +1.2 2,927,287 - RPSI07_RS21225 0.52 -0.3 2,927,289 + RPSI07_RS21225 0.53 +0.9 2,927,289 + RPSI07_RS21225 0.53 +1.2 2,927,290 - RPSI07_RS21225 0.53 +0.4 2,927,290 - RPSI07_RS21225 0.53 +0.1 2,927,290 - RPSI07_RS21225 0.53 +0.3 2,927,311 - RPSI07_RS21225 0.56 +0.0 2,927,412 + RPSI07_RS21225 0.72 +0.2 2,927,413 - RPSI07_RS21225 0.72 -0.5 2,927,413 - RPSI07_RS21225 0.72 -0.2 2,927,430 + RPSI07_RS21225 0.75 +1.2 2,927,469 + RPSI07_RS21225 0.81 -0.4 2,927,470 - RPSI07_RS21225 0.81 -0.6 2,927,470 - RPSI07_RS21225 0.81 -0.1 2,927,470 - RPSI07_RS21225 0.81 +0.0 2,927,531 + +1.0 2,927,627 + -0.6 2,927,667 + -0.7 2,927,668 - +1.2 2,927,668 - +0.2 2,927,668 - -0.4 2,927,822 + RPSI07_RS21230 0.34 +0.4 2,927,823 - RPSI07_RS21230 0.34 -1.9 2,927,830 + RPSI07_RS21230 0.35 +1.2 2,927,830 + RPSI07_RS21230 0.35 -0.9 2,927,862 + RPSI07_RS21230 0.42 +0.2 2,927,872 + RPSI07_RS21230 0.43 -0.9 2,927,873 - RPSI07_RS21230 0.44 -0.4 2,927,953 + RPSI07_RS21230 0.59 -2.4 2,927,953 + RPSI07_RS21230 0.59 -1.1 2,927,953 + RPSI07_RS21230 0.59 -0.3 2,927,953 + RPSI07_RS21230 0.59 -1.2 2,927,954 - RPSI07_RS21230 0.59 +0.2 2,927,954 - RPSI07_RS21230 0.59 -0.3 2,928,042 + RPSI07_RS21230 0.76 -2.1 2,928,043 - RPSI07_RS21230 0.76 -0.3 2,928,052 - RPSI07_RS21230 0.78 -0.5 2,928,077 + RPSI07_RS21230 0.83 -0.7 2,928,191 + -0.2 2,928,280 + -0.0 2,928,281 - +1.6 2,928,514 + RPSI07_RS21235 0.22 +0.5 2,928,515 - RPSI07_RS21235 0.22 -0.4 2,928,811 + RPSI07_RS21235 0.65 +0.5 2,928,811 + RPSI07_RS21235 0.65 -0.5 2,928,845 - RPSI07_RS21235 0.69 -0.4 2,929,113 + -0.8 2,929,114 - +1.2 2,929,115 + -0.7 2,929,115 + +0.2 2,929,115 + -0.5 2,929,115 + +0.4 2,929,115 + -0.1 2,929,116 - +1.2
Or see this region's nucleotide sequence