Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS19530

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS19525 and RPSI07_RS19530 overlap by 4 nucleotidesRPSI07_RS19530 and RPSI07_RS19535 are separated by 24 nucleotidesRPSI07_RS19535 and RPSI07_RS19540 are separated by 7 nucleotides RPSI07_RS19525: RPSI07_RS19525 - peptidase M48, at 2,563,494 to 2,564,750 _RS19525 RPSI07_RS19530: RPSI07_RS19530 - ribosome small subunit-dependent GTPase A, at 2,564,747 to 2,565,703 _RS19530 RPSI07_RS19535: RPSI07_RS19535 - hypothetical protein, at 2,565,728 to 2,566,042 _RS19535 RPSI07_RS19540: RPSI07_RS19540 - threonine-phosphate decarboxylase, at 2,566,050 to 2,566,991 _RS19540 Position (kb) 2564 2565 2566Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 2563.795 kb on - strand, within RPSI07_RS19525at 2563.860 kb on + strand, within RPSI07_RS19525at 2564.025 kb on + strand, within RPSI07_RS19525at 2564.073 kb on + strand, within RPSI07_RS19525at 2564.097 kb on + strand, within RPSI07_RS19525at 2564.097 kb on + strand, within RPSI07_RS19525at 2564.436 kb on + strand, within RPSI07_RS19525at 2564.436 kb on + strand, within RPSI07_RS19525at 2564.436 kb on + strand, within RPSI07_RS19525at 2564.437 kb on - strand, within RPSI07_RS19525at 2564.679 kb on + strandat 2564.679 kb on + strandat 2564.691 kb on + strandat 2564.692 kb on - strandat 2564.694 kb on + strandat 2565.428 kb on + strand, within RPSI07_RS19530at 2565.429 kb on - strand, within RPSI07_RS19530at 2565.825 kb on - strand, within RPSI07_RS19535at 2565.876 kb on + strand, within RPSI07_RS19535at 2565.876 kb on + strand, within RPSI07_RS19535at 2565.877 kb on - strand, within RPSI07_RS19535at 2566.324 kb on + strand, within RPSI07_RS19540at 2566.325 kb on - strand, within RPSI07_RS19540at 2566.325 kb on - strand, within RPSI07_RS19540at 2566.387 kb on + strand, within RPSI07_RS19540at 2566.387 kb on + strand, within RPSI07_RS19540at 2566.387 kb on + strand, within RPSI07_RS19540at 2566.387 kb on + strand, within RPSI07_RS19540at 2566.388 kb on - strand, within RPSI07_RS19540at 2566.388 kb on - strand, within RPSI07_RS19540at 2566.437 kb on - strand, within RPSI07_RS19540at 2566.474 kb on + strand, within RPSI07_RS19540at 2566.610 kb on + strand, within RPSI07_RS19540at 2566.610 kb on + strand, within RPSI07_RS19540at 2566.702 kb on + strand, within RPSI07_RS19540at 2566.702 kb on + strand, within RPSI07_RS19540at 2566.702 kb on + strand, within RPSI07_RS19540at 2566.702 kb on + strand, within RPSI07_RS19540

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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2,563,795 - RPSI07_RS19525 0.24 +1.1
2,563,860 + RPSI07_RS19525 0.29 +0.8
2,564,025 + RPSI07_RS19525 0.42 -0.3
2,564,073 + RPSI07_RS19525 0.46 -0.0
2,564,097 + RPSI07_RS19525 0.48 -0.5
2,564,097 + RPSI07_RS19525 0.48 +0.0
2,564,436 + RPSI07_RS19525 0.75 -0.9
2,564,436 + RPSI07_RS19525 0.75 +1.1
2,564,436 + RPSI07_RS19525 0.75 +1.8
2,564,437 - RPSI07_RS19525 0.75 +0.1
2,564,679 + +0.1
2,564,679 + -0.2
2,564,691 + -0.9
2,564,692 - -1.7
2,564,694 + -1.6
2,565,428 + RPSI07_RS19530 0.71 -0.9
2,565,429 - RPSI07_RS19530 0.71 +1.1
2,565,825 - RPSI07_RS19535 0.31 +1.1
2,565,876 + RPSI07_RS19535 0.47 -0.5
2,565,876 + RPSI07_RS19535 0.47 -0.3
2,565,877 - RPSI07_RS19535 0.47 +0.1
2,566,324 + RPSI07_RS19540 0.29 -4.8
2,566,325 - RPSI07_RS19540 0.29 -4.3
2,566,325 - RPSI07_RS19540 0.29 -4.2
2,566,387 + RPSI07_RS19540 0.36 -3.8
2,566,387 + RPSI07_RS19540 0.36 -3.9
2,566,387 + RPSI07_RS19540 0.36 -4.1
2,566,387 + RPSI07_RS19540 0.36 -4.6
2,566,388 - RPSI07_RS19540 0.36 -4.1
2,566,388 - RPSI07_RS19540 0.36 -4.3
2,566,437 - RPSI07_RS19540 0.41 -3.8
2,566,474 + RPSI07_RS19540 0.45 -3.9
2,566,610 + RPSI07_RS19540 0.59 -4.2
2,566,610 + RPSI07_RS19540 0.59 -3.9
2,566,702 + RPSI07_RS19540 0.69 +1.1
2,566,702 + RPSI07_RS19540 0.69 -4.0
2,566,702 + RPSI07_RS19540 0.69 -4.1
2,566,702 + RPSI07_RS19540 0.69 -3.8

Or see this region's nucleotide sequence