Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS19525 and RPSI07_RS19530 overlap by 4 nucleotides RPSI07_RS19530 and RPSI07_RS19535 are separated by 24 nucleotides RPSI07_RS19535 and RPSI07_RS19540 are separated by 7 nucleotides
RPSI07_RS19525: RPSI07_RS19525 - peptidase M48, at 2,563,494 to 2,564,750
_RS19525
RPSI07_RS19530: RPSI07_RS19530 - ribosome small subunit-dependent GTPase A, at 2,564,747 to 2,565,703
_RS19530
RPSI07_RS19535: RPSI07_RS19535 - hypothetical protein, at 2,565,728 to 2,566,042
_RS19535
RPSI07_RS19540: RPSI07_RS19540 - threonine-phosphate decarboxylase, at 2,566,050 to 2,566,991
_RS19540
Position (kb)
2564
2565
2566 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2563.795 kb on - strand, within RPSI07_RS19525 at 2563.860 kb on + strand, within RPSI07_RS19525 at 2564.025 kb on + strand, within RPSI07_RS19525 at 2564.073 kb on + strand, within RPSI07_RS19525 at 2564.097 kb on + strand, within RPSI07_RS19525 at 2564.097 kb on + strand, within RPSI07_RS19525 at 2564.436 kb on + strand, within RPSI07_RS19525 at 2564.436 kb on + strand, within RPSI07_RS19525 at 2564.436 kb on + strand, within RPSI07_RS19525 at 2564.437 kb on - strand, within RPSI07_RS19525 at 2564.679 kb on + strand at 2564.679 kb on + strand at 2564.691 kb on + strand at 2564.692 kb on - strand at 2564.694 kb on + strand at 2565.428 kb on + strand, within RPSI07_RS19530 at 2565.429 kb on - strand, within RPSI07_RS19530 at 2565.825 kb on - strand, within RPSI07_RS19535 at 2565.876 kb on + strand, within RPSI07_RS19535 at 2565.876 kb on + strand, within RPSI07_RS19535 at 2565.877 kb on - strand, within RPSI07_RS19535 at 2566.324 kb on + strand, within RPSI07_RS19540 at 2566.325 kb on - strand, within RPSI07_RS19540 at 2566.325 kb on - strand, within RPSI07_RS19540 at 2566.387 kb on + strand, within RPSI07_RS19540 at 2566.387 kb on + strand, within RPSI07_RS19540 at 2566.387 kb on + strand, within RPSI07_RS19540 at 2566.387 kb on + strand, within RPSI07_RS19540 at 2566.388 kb on - strand, within RPSI07_RS19540 at 2566.388 kb on - strand, within RPSI07_RS19540 at 2566.437 kb on - strand, within RPSI07_RS19540 at 2566.474 kb on + strand, within RPSI07_RS19540 at 2566.610 kb on + strand, within RPSI07_RS19540 at 2566.610 kb on + strand, within RPSI07_RS19540 at 2566.702 kb on + strand, within RPSI07_RS19540 at 2566.702 kb on + strand, within RPSI07_RS19540 at 2566.702 kb on + strand, within RPSI07_RS19540 at 2566.702 kb on + strand, within RPSI07_RS19540
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 2,563,795 - RPSI07_RS19525 0.24 +1.1 2,563,860 + RPSI07_RS19525 0.29 +0.8 2,564,025 + RPSI07_RS19525 0.42 -0.3 2,564,073 + RPSI07_RS19525 0.46 -0.0 2,564,097 + RPSI07_RS19525 0.48 -0.5 2,564,097 + RPSI07_RS19525 0.48 +0.0 2,564,436 + RPSI07_RS19525 0.75 -0.9 2,564,436 + RPSI07_RS19525 0.75 +1.1 2,564,436 + RPSI07_RS19525 0.75 +1.8 2,564,437 - RPSI07_RS19525 0.75 +0.1 2,564,679 + +0.1 2,564,679 + -0.2 2,564,691 + -0.9 2,564,692 - -1.7 2,564,694 + -1.6 2,565,428 + RPSI07_RS19530 0.71 -0.9 2,565,429 - RPSI07_RS19530 0.71 +1.1 2,565,825 - RPSI07_RS19535 0.31 +1.1 2,565,876 + RPSI07_RS19535 0.47 -0.5 2,565,876 + RPSI07_RS19535 0.47 -0.3 2,565,877 - RPSI07_RS19535 0.47 +0.1 2,566,324 + RPSI07_RS19540 0.29 -4.8 2,566,325 - RPSI07_RS19540 0.29 -4.3 2,566,325 - RPSI07_RS19540 0.29 -4.2 2,566,387 + RPSI07_RS19540 0.36 -3.8 2,566,387 + RPSI07_RS19540 0.36 -3.9 2,566,387 + RPSI07_RS19540 0.36 -4.1 2,566,387 + RPSI07_RS19540 0.36 -4.6 2,566,388 - RPSI07_RS19540 0.36 -4.1 2,566,388 - RPSI07_RS19540 0.36 -4.3 2,566,437 - RPSI07_RS19540 0.41 -3.8 2,566,474 + RPSI07_RS19540 0.45 -3.9 2,566,610 + RPSI07_RS19540 0.59 -4.2 2,566,610 + RPSI07_RS19540 0.59 -3.9 2,566,702 + RPSI07_RS19540 0.69 +1.1 2,566,702 + RPSI07_RS19540 0.69 -4.0 2,566,702 + RPSI07_RS19540 0.69 -4.1 2,566,702 + RPSI07_RS19540 0.69 -3.8
Or see this region's nucleotide sequence