Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS07265 and RPSI07_RS07270 are separated by 167 nucleotides RPSI07_RS07270 and RPSI07_RS07275 are separated by 92 nucleotides RPSI07_RS07275 and RPSI07_RS07280 are separated by 196 nucleotides
RPSI07_RS07265: RPSI07_RS07265 - hypothetical protein, at 1,930,465 to 1,930,794
_RS07265
RPSI07_RS07270: RPSI07_RS07270 - DUF2059 domain-containing protein, at 1,930,962 to 1,931,483
_RS07270
RPSI07_RS07275: RPSI07_RS07275 - hypothetical protein, at 1,931,576 to 1,931,926
_RS07275
RPSI07_RS07280: RPSI07_RS07280 - PDZ domain-containing protein, at 1,932,123 to 1,933,595
_RS07280
Position (kb) 1931
1932 Strain fitness (log2 ratio) -1
0
1
2 at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.640 kb on + strand, within RPSI07_RS07265 at 1930.640 kb on + strand, within RPSI07_RS07265 at 1930.641 kb on - strand, within RPSI07_RS07265 at 1930.641 kb on - strand, within RPSI07_RS07265 at 1930.822 kb on + strand at 1930.823 kb on - strand at 1930.963 kb on + strand at 1930.964 kb on - strand at 1930.964 kb on - strand at 1930.964 kb on - strand at 1930.964 kb on - strand at 1930.964 kb on - strand at 1930.964 kb on - strand at 1930.964 kb on - strand at 1930.968 kb on - strand at 1931.044 kb on + strand, within RPSI07_RS07270 at 1931.044 kb on + strand, within RPSI07_RS07270 at 1931.045 kb on - strand, within RPSI07_RS07270 at 1931.045 kb on - strand, within RPSI07_RS07270 at 1931.045 kb on - strand, within RPSI07_RS07270 at 1931.045 kb on - strand, within RPSI07_RS07270 at 1931.045 kb on - strand, within RPSI07_RS07270 at 1931.123 kb on + strand, within RPSI07_RS07270 at 1931.123 kb on + strand, within RPSI07_RS07270 at 1931.123 kb on + strand, within RPSI07_RS07270 at 1931.124 kb on - strand, within RPSI07_RS07270 at 1931.124 kb on - strand, within RPSI07_RS07270 at 1931.124 kb on - strand, within RPSI07_RS07270 at 1931.124 kb on - strand, within RPSI07_RS07270 at 1931.124 kb on - strand, within RPSI07_RS07270 at 1931.124 kb on - strand, within RPSI07_RS07270 at 1931.124 kb on - strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on - strand, within RPSI07_RS07270 at 1931.126 kb on - strand, within RPSI07_RS07270 at 1931.126 kb on - strand, within RPSI07_RS07270 at 1931.126 kb on - strand, within RPSI07_RS07270 at 1931.126 kb on - strand, within RPSI07_RS07270 at 1931.126 kb on - strand, within RPSI07_RS07270 at 1931.270 kb on - strand, within RPSI07_RS07270 at 1931.478 kb on + strand at 1931.505 kb on + strand at 1931.524 kb on + strand at 1931.524 kb on + strand at 1931.525 kb on - strand at 1931.525 kb on - strand at 1931.525 kb on - strand at 1931.525 kb on - strand at 1931.525 kb on - strand at 1931.525 kb on - strand at 1931.525 kb on - strand at 1931.525 kb on - strand at 1931.556 kb on + strand at 1931.557 kb on - strand at 1931.686 kb on - strand, within RPSI07_RS07275 at 1931.754 kb on + strand, within RPSI07_RS07275 at 1931.755 kb on - strand, within RPSI07_RS07275 at 1931.757 kb on + strand, within RPSI07_RS07275 at 1931.757 kb on + strand, within RPSI07_RS07275 at 1931.757 kb on + strand, within RPSI07_RS07275 at 1931.757 kb on + strand, within RPSI07_RS07275 at 1931.757 kb on + strand, within RPSI07_RS07275 at 1931.758 kb on - strand, within RPSI07_RS07275 at 1931.758 kb on - strand, within RPSI07_RS07275 at 1931.846 kb on + strand, within RPSI07_RS07275 at 1931.847 kb on - strand, within RPSI07_RS07275 at 1931.916 kb on + strand at 1931.916 kb on + strand at 1931.916 kb on + strand at 1931.995 kb on - strand at 1931.995 kb on - strand at 1931.995 kb on - strand at 1932.799 kb on + strand, within RPSI07_RS07280 at 1932.817 kb on + strand, within RPSI07_RS07280 at 1932.817 kb on + strand, within RPSI07_RS07280 at 1932.817 kb on + strand, within RPSI07_RS07280 at 1932.817 kb on + strand, within RPSI07_RS07280 at 1932.817 kb on + strand, within RPSI07_RS07280 at 1932.817 kb on + strand, within RPSI07_RS07280 at 1932.818 kb on - strand at 1932.919 kb on + strand, within RPSI07_RS07280 at 1932.919 kb on + strand, within RPSI07_RS07280 at 1932.919 kb on + strand, within RPSI07_RS07280 at 1932.919 kb on + strand, within RPSI07_RS07280 at 1932.919 kb on + strand, within RPSI07_RS07280 at 1932.919 kb on + strand, within RPSI07_RS07280 at 1932.919 kb on + strand, within RPSI07_RS07280 at 1932.919 kb on + strand, within RPSI07_RS07280 at 1932.919 kb on + strand, within RPSI07_RS07280 at 1932.919 kb on + strand, within RPSI07_RS07280 at 1932.919 kb on + strand, within RPSI07_RS07280 at 1932.919 kb on + strand, within RPSI07_RS07280 at 1932.919 kb on + strand at 1932.920 kb on - strand, within RPSI07_RS07280 at 1932.920 kb on - strand, within RPSI07_RS07280 at 1932.920 kb on - strand, within RPSI07_RS07280 at 1932.920 kb on - strand, within RPSI07_RS07280 at 1932.920 kb on - strand, within RPSI07_RS07280 at 1932.920 kb on - strand, within RPSI07_RS07280 at 1932.920 kb on - strand, within RPSI07_RS07280 at 1932.920 kb on - strand, within RPSI07_RS07280
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 1,930,610 + RPSI07_RS07265 0.44 -0.9 1,930,610 + RPSI07_RS07265 0.44 -1.0 1,930,610 + RPSI07_RS07265 0.44 +0.0 1,930,610 + RPSI07_RS07265 0.44 -0.5 1,930,610 + RPSI07_RS07265 0.44 -0.3 1,930,610 + RPSI07_RS07265 0.44 +0.9 1,930,610 + RPSI07_RS07265 0.44 -0.4 1,930,610 + RPSI07_RS07265 0.44 -0.9 1,930,611 - RPSI07_RS07265 0.44 -0.3 1,930,611 - RPSI07_RS07265 0.44 -0.3 1,930,611 - RPSI07_RS07265 0.44 +0.4 1,930,611 - RPSI07_RS07265 0.44 -1.1 1,930,611 - RPSI07_RS07265 0.44 -0.4 1,930,611 - RPSI07_RS07265 0.44 +0.6 1,930,611 - RPSI07_RS07265 0.44 -0.9 1,930,611 - RPSI07_RS07265 0.44 -0.8 1,930,611 - RPSI07_RS07265 0.44 -0.4 1,930,611 - RPSI07_RS07265 0.44 +0.5 1,930,611 - RPSI07_RS07265 0.44 -0.2 1,930,611 - RPSI07_RS07265 0.44 -0.6 1,930,611 - RPSI07_RS07265 0.44 -0.9 1,930,640 + RPSI07_RS07265 0.53 +0.3 1,930,640 + RPSI07_RS07265 0.53 +0.3 1,930,641 - RPSI07_RS07265 0.53 +1.2 1,930,641 - RPSI07_RS07265 0.53 +0.4 1,930,822 + +0.6 1,930,823 - -1.5 1,930,963 + +0.9 1,930,964 - +0.9 1,930,964 - +0.6 1,930,964 - -0.7 1,930,964 - -0.6 1,930,964 - -0.2 1,930,964 - -0.2 1,930,964 - +0.9 1,930,968 - -1.4 1,931,044 + RPSI07_RS07270 0.16 -0.0 1,931,044 + RPSI07_RS07270 0.16 +0.9 1,931,045 - RPSI07_RS07270 0.16 +0.9 1,931,045 - RPSI07_RS07270 0.16 +0.9 1,931,045 - RPSI07_RS07270 0.16 +0.4 1,931,045 - RPSI07_RS07270 0.16 +0.0 1,931,045 - RPSI07_RS07270 0.16 +0.3 1,931,123 + RPSI07_RS07270 0.31 -1.4 1,931,123 + RPSI07_RS07270 0.31 -0.6 1,931,123 + RPSI07_RS07270 0.31 -0.3 1,931,124 - RPSI07_RS07270 0.31 -0.2 1,931,124 - RPSI07_RS07270 0.31 +0.3 1,931,124 - RPSI07_RS07270 0.31 -0.3 1,931,124 - RPSI07_RS07270 0.31 +0.2 1,931,124 - RPSI07_RS07270 0.31 +0.9 1,931,124 - RPSI07_RS07270 0.31 -1.1 1,931,124 - RPSI07_RS07270 0.31 +0.1 1,931,125 + RPSI07_RS07270 0.31 -0.8 1,931,125 + RPSI07_RS07270 0.31 -0.4 1,931,125 + RPSI07_RS07270 0.31 +0.9 1,931,125 + RPSI07_RS07270 0.31 +0.4 1,931,125 + RPSI07_RS07270 0.31 +0.8 1,931,125 + RPSI07_RS07270 0.31 -0.6 1,931,125 + RPSI07_RS07270 0.31 -1.1 1,931,125 + RPSI07_RS07270 0.31 -1.6 1,931,125 + RPSI07_RS07270 0.31 -1.7 1,931,125 + RPSI07_RS07270 0.31 -1.0 1,931,125 + RPSI07_RS07270 0.31 +0.1 1,931,125 + RPSI07_RS07270 0.31 +0.5 1,931,125 - RPSI07_RS07270 0.31 -0.5 1,931,126 - RPSI07_RS07270 0.31 +1.1 1,931,126 - RPSI07_RS07270 0.31 +1.4 1,931,126 - RPSI07_RS07270 0.31 -0.1 1,931,126 - RPSI07_RS07270 0.31 +0.2 1,931,126 - RPSI07_RS07270 0.31 -0.4 1,931,270 - RPSI07_RS07270 0.59 +0.1 1,931,478 + +1.1 1,931,505 + +0.3 1,931,524 + +0.4 1,931,524 + -0.3 1,931,525 - +0.0 1,931,525 - -0.1 1,931,525 - -0.1 1,931,525 - +0.0 1,931,525 - +0.5 1,931,525 - -0.1 1,931,525 - +1.9 1,931,525 - -1.1 1,931,556 + +0.7 1,931,557 - -0.2 1,931,686 - RPSI07_RS07275 0.31 +1.5 1,931,754 + RPSI07_RS07275 0.51 +0.4 1,931,755 - RPSI07_RS07275 0.51 +1.1 1,931,757 + RPSI07_RS07275 0.52 +2.0 1,931,757 + RPSI07_RS07275 0.52 +1.5 1,931,757 + RPSI07_RS07275 0.52 -0.1 1,931,757 + RPSI07_RS07275 0.52 +1.4 1,931,757 + RPSI07_RS07275 0.52 +0.8 1,931,758 - RPSI07_RS07275 0.52 -0.1 1,931,758 - RPSI07_RS07275 0.52 +1.6 1,931,846 + RPSI07_RS07275 0.77 +1.3 1,931,847 - RPSI07_RS07275 0.77 +1.6 1,931,916 + +0.6 1,931,916 + +1.6 1,931,916 + +0.9 1,931,995 - -1.3 1,931,995 - -0.4 1,931,995 - -0.2 1,932,799 + RPSI07_RS07280 0.46 +2.7 1,932,817 + RPSI07_RS07280 0.47 -0.2 1,932,817 + RPSI07_RS07280 0.47 -0.1 1,932,817 + RPSI07_RS07280 0.47 +0.1 1,932,817 + RPSI07_RS07280 0.47 -0.4 1,932,817 + RPSI07_RS07280 0.47 +1.4 1,932,817 + RPSI07_RS07280 0.47 -0.1 1,932,818 - -0.5 1,932,919 + RPSI07_RS07280 0.54 -0.6 1,932,919 + RPSI07_RS07280 0.54 -0.9 1,932,919 + RPSI07_RS07280 0.54 -1.1 1,932,919 + RPSI07_RS07280 0.54 -0.9 1,932,919 + RPSI07_RS07280 0.54 +0.9 1,932,919 + RPSI07_RS07280 0.54 -0.6 1,932,919 + RPSI07_RS07280 0.54 -0.6 1,932,919 + RPSI07_RS07280 0.54 -1.1 1,932,919 + RPSI07_RS07280 0.54 -0.3 1,932,919 + RPSI07_RS07280 0.54 -1.7 1,932,919 + RPSI07_RS07280 0.54 -0.9 1,932,919 + RPSI07_RS07280 0.54 -0.3 1,932,919 + +0.9 1,932,920 - RPSI07_RS07280 0.54 +0.1 1,932,920 - RPSI07_RS07280 0.54 +0.2 1,932,920 - RPSI07_RS07280 0.54 +0.0 1,932,920 - RPSI07_RS07280 0.54 +0.2 1,932,920 - RPSI07_RS07280 0.54 -0.2 1,932,920 - RPSI07_RS07280 0.54 -0.4 1,932,920 - RPSI07_RS07280 0.54 -0.6 1,932,920 - RPSI07_RS07280 0.54 +1.3
Or see this region's nucleotide sequence