Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS07260 and RPSI07_RS07265 are separated by 436 nucleotides RPSI07_RS07265 and RPSI07_RS07270 are separated by 167 nucleotides RPSI07_RS07270 and RPSI07_RS07275 are separated by 92 nucleotides
RPSI07_RS07260: RPSI07_RS07260 - hypothetical protein, at 1,929,432 to 1,930,028
_RS07260
RPSI07_RS07265: RPSI07_RS07265 - hypothetical protein, at 1,930,465 to 1,930,794
_RS07265
RPSI07_RS07270: RPSI07_RS07270 - DUF2059 domain-containing protein, at 1,930,962 to 1,931,483
_RS07270
RPSI07_RS07275: RPSI07_RS07275 - hypothetical protein, at 1,931,576 to 1,931,926
_RS07275
Position (kb) 1930
1931 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1929.466 kb on + strand at 1929.466 kb on + strand at 1929.466 kb on + strand at 1929.466 kb on + strand at 1929.487 kb on + strand at 1929.487 kb on + strand at 1929.523 kb on + strand, within RPSI07_RS07260 at 1929.523 kb on + strand, within RPSI07_RS07260 at 1929.524 kb on - strand, within RPSI07_RS07260 at 1929.524 kb on - strand, within RPSI07_RS07260 at 1929.524 kb on - strand, within RPSI07_RS07260 at 1929.581 kb on + strand, within RPSI07_RS07260 at 1929.581 kb on + strand, within RPSI07_RS07260 at 1929.581 kb on + strand, within RPSI07_RS07260 at 1929.582 kb on - strand, within RPSI07_RS07260 at 1929.582 kb on - strand, within RPSI07_RS07260 at 1929.582 kb on - strand, within RPSI07_RS07260 at 1929.582 kb on - strand, within RPSI07_RS07260 at 1929.594 kb on + strand, within RPSI07_RS07260 at 1929.594 kb on + strand, within RPSI07_RS07260 at 1929.594 kb on + strand, within RPSI07_RS07260 at 1929.594 kb on + strand, within RPSI07_RS07260 at 1929.595 kb on - strand, within RPSI07_RS07260 at 1929.595 kb on - strand, within RPSI07_RS07260 at 1929.595 kb on - strand, within RPSI07_RS07260 at 1929.595 kb on - strand, within RPSI07_RS07260 at 1929.622 kb on + strand, within RPSI07_RS07260 at 1929.623 kb on - strand, within RPSI07_RS07260 at 1929.623 kb on - strand, within RPSI07_RS07260 at 1929.627 kb on + strand, within RPSI07_RS07260 at 1929.627 kb on + strand, within RPSI07_RS07260 at 1929.627 kb on + strand, within RPSI07_RS07260 at 1929.628 kb on - strand, within RPSI07_RS07260 at 1929.628 kb on - strand, within RPSI07_RS07260 at 1929.628 kb on - strand, within RPSI07_RS07260 at 1929.628 kb on - strand, within RPSI07_RS07260 at 1929.687 kb on + strand, within RPSI07_RS07260 at 1929.687 kb on + strand, within RPSI07_RS07260 at 1929.688 kb on - strand, within RPSI07_RS07260 at 1929.688 kb on - strand, within RPSI07_RS07260 at 1929.688 kb on - strand, within RPSI07_RS07260 at 1929.688 kb on - strand, within RPSI07_RS07260 at 1929.688 kb on - strand, within RPSI07_RS07260 at 1929.736 kb on + strand, within RPSI07_RS07260 at 1929.737 kb on - strand, within RPSI07_RS07260 at 1929.737 kb on - strand, within RPSI07_RS07260 at 1929.780 kb on + strand, within RPSI07_RS07260 at 1929.781 kb on + strand, within RPSI07_RS07260 at 1929.782 kb on - strand, within RPSI07_RS07260 at 1929.782 kb on - strand, within RPSI07_RS07260 at 1929.782 kb on - strand, within RPSI07_RS07260 at 1929.799 kb on + strand, within RPSI07_RS07260 at 1929.800 kb on - strand, within RPSI07_RS07260 at 1929.812 kb on + strand, within RPSI07_RS07260 at 1929.812 kb on + strand, within RPSI07_RS07260 at 1929.812 kb on + strand, within RPSI07_RS07260 at 1929.812 kb on + strand, within RPSI07_RS07260 at 1929.812 kb on + strand, within RPSI07_RS07260 at 1929.812 kb on + strand, within RPSI07_RS07260 at 1929.812 kb on + strand, within RPSI07_RS07260 at 1929.813 kb on - strand, within RPSI07_RS07260 at 1929.813 kb on - strand, within RPSI07_RS07260 at 1929.813 kb on - strand, within RPSI07_RS07260 at 1929.813 kb on - strand, within RPSI07_RS07260 at 1929.814 kb on + strand, within RPSI07_RS07260 at 1929.814 kb on + strand, within RPSI07_RS07260 at 1929.814 kb on + strand, within RPSI07_RS07260 at 1929.814 kb on + strand, within RPSI07_RS07260 at 1929.814 kb on + strand, within RPSI07_RS07260 at 1929.814 kb on + strand, within RPSI07_RS07260 at 1929.814 kb on + strand, within RPSI07_RS07260 at 1929.814 kb on + strand, within RPSI07_RS07260 at 1929.815 kb on - strand, within RPSI07_RS07260 at 1929.815 kb on - strand, within RPSI07_RS07260 at 1929.815 kb on - strand, within RPSI07_RS07260 at 1929.815 kb on - strand, within RPSI07_RS07260 at 1929.815 kb on - strand, within RPSI07_RS07260 at 1929.815 kb on - strand, within RPSI07_RS07260 at 1929.815 kb on - strand, within RPSI07_RS07260 at 1929.816 kb on - strand, within RPSI07_RS07260 at 1929.816 kb on - strand, within RPSI07_RS07260 at 1929.869 kb on - strand, within RPSI07_RS07260 at 1930.063 kb on + strand at 1930.063 kb on + strand at 1930.063 kb on + strand at 1930.063 kb on + strand at 1930.063 kb on + strand at 1930.064 kb on - strand at 1930.083 kb on + strand at 1930.083 kb on + strand at 1930.084 kb on - strand at 1930.084 kb on - strand at 1930.094 kb on + strand at 1930.095 kb on - strand at 1930.318 kb on + strand at 1930.318 kb on + strand at 1930.318 kb on + strand at 1930.318 kb on + strand at 1930.319 kb on - strand at 1930.319 kb on - strand at 1930.319 kb on - strand at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.610 kb on + strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.611 kb on - strand, within RPSI07_RS07265 at 1930.640 kb on + strand, within RPSI07_RS07265 at 1930.640 kb on + strand, within RPSI07_RS07265 at 1930.641 kb on - strand, within RPSI07_RS07265 at 1930.641 kb on - strand, within RPSI07_RS07265 at 1930.822 kb on + strand at 1930.823 kb on - strand at 1930.963 kb on + strand at 1930.964 kb on - strand at 1930.964 kb on - strand at 1930.964 kb on - strand at 1930.964 kb on - strand at 1930.964 kb on - strand at 1930.964 kb on - strand at 1930.964 kb on - strand at 1930.968 kb on - strand at 1931.044 kb on + strand, within RPSI07_RS07270 at 1931.044 kb on + strand, within RPSI07_RS07270 at 1931.045 kb on - strand, within RPSI07_RS07270 at 1931.045 kb on - strand, within RPSI07_RS07270 at 1931.045 kb on - strand, within RPSI07_RS07270 at 1931.045 kb on - strand, within RPSI07_RS07270 at 1931.045 kb on - strand, within RPSI07_RS07270 at 1931.123 kb on + strand, within RPSI07_RS07270 at 1931.123 kb on + strand, within RPSI07_RS07270 at 1931.123 kb on + strand, within RPSI07_RS07270 at 1931.124 kb on - strand, within RPSI07_RS07270 at 1931.124 kb on - strand, within RPSI07_RS07270 at 1931.124 kb on - strand, within RPSI07_RS07270 at 1931.124 kb on - strand, within RPSI07_RS07270 at 1931.124 kb on - strand, within RPSI07_RS07270 at 1931.124 kb on - strand, within RPSI07_RS07270 at 1931.124 kb on - strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on + strand, within RPSI07_RS07270 at 1931.125 kb on - strand, within RPSI07_RS07270 at 1931.126 kb on - strand, within RPSI07_RS07270 at 1931.126 kb on - strand, within RPSI07_RS07270 at 1931.126 kb on - strand, within RPSI07_RS07270 at 1931.126 kb on - strand, within RPSI07_RS07270 at 1931.126 kb on - strand, within RPSI07_RS07270 at 1931.270 kb on - strand, within RPSI07_RS07270 at 1931.478 kb on + strand at 1931.505 kb on + strand at 1931.524 kb on + strand at 1931.524 kb on + strand at 1931.525 kb on - strand at 1931.525 kb on - strand at 1931.525 kb on - strand at 1931.525 kb on - strand at 1931.525 kb on - strand at 1931.525 kb on - strand at 1931.525 kb on - strand at 1931.525 kb on - strand at 1931.556 kb on + strand at 1931.557 kb on - strand at 1931.686 kb on - strand, within RPSI07_RS07275 at 1931.754 kb on + strand, within RPSI07_RS07275 at 1931.755 kb on - strand, within RPSI07_RS07275 at 1931.757 kb on + strand, within RPSI07_RS07275 at 1931.757 kb on + strand, within RPSI07_RS07275 at 1931.757 kb on + strand, within RPSI07_RS07275 at 1931.757 kb on + strand, within RPSI07_RS07275 at 1931.757 kb on + strand, within RPSI07_RS07275 at 1931.758 kb on - strand, within RPSI07_RS07275 at 1931.758 kb on - strand, within RPSI07_RS07275
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 1,929,466 + -0.2 1,929,466 + -0.4 1,929,466 + -0.6 1,929,466 + -1.4 1,929,487 + -0.9 1,929,487 + -0.3 1,929,523 + RPSI07_RS07260 0.15 +0.2 1,929,523 + RPSI07_RS07260 0.15 +0.2 1,929,524 - RPSI07_RS07260 0.15 -0.6 1,929,524 - RPSI07_RS07260 0.15 -0.7 1,929,524 - RPSI07_RS07260 0.15 -0.3 1,929,581 + RPSI07_RS07260 0.25 -0.5 1,929,581 + RPSI07_RS07260 0.25 -0.5 1,929,581 + RPSI07_RS07260 0.25 -0.4 1,929,582 - RPSI07_RS07260 0.25 -0.4 1,929,582 - RPSI07_RS07260 0.25 +0.2 1,929,582 - RPSI07_RS07260 0.25 -1.4 1,929,582 - RPSI07_RS07260 0.25 -1.1 1,929,594 + RPSI07_RS07260 0.27 -0.4 1,929,594 + RPSI07_RS07260 0.27 -0.1 1,929,594 + RPSI07_RS07260 0.27 -0.4 1,929,594 + RPSI07_RS07260 0.27 -1.5 1,929,595 - RPSI07_RS07260 0.27 +0.2 1,929,595 - RPSI07_RS07260 0.27 -0.7 1,929,595 - RPSI07_RS07260 0.27 +0.9 1,929,595 - RPSI07_RS07260 0.27 +0.9 1,929,622 + RPSI07_RS07260 0.32 -0.6 1,929,623 - RPSI07_RS07260 0.32 -0.4 1,929,623 - RPSI07_RS07260 0.32 -0.5 1,929,627 + RPSI07_RS07260 0.33 -0.5 1,929,627 + RPSI07_RS07260 0.33 -2.5 1,929,627 + RPSI07_RS07260 0.33 +0.2 1,929,628 - RPSI07_RS07260 0.33 -0.8 1,929,628 - RPSI07_RS07260 0.33 -0.1 1,929,628 - RPSI07_RS07260 0.33 +0.6 1,929,628 - RPSI07_RS07260 0.33 +0.1 1,929,687 + RPSI07_RS07260 0.43 +0.9 1,929,687 + RPSI07_RS07260 0.43 -0.2 1,929,688 - RPSI07_RS07260 0.43 -1.2 1,929,688 - RPSI07_RS07260 0.43 -0.8 1,929,688 - RPSI07_RS07260 0.43 +0.1 1,929,688 - RPSI07_RS07260 0.43 -0.5 1,929,688 - RPSI07_RS07260 0.43 -0.5 1,929,736 + RPSI07_RS07260 0.51 -0.8 1,929,737 - RPSI07_RS07260 0.51 -1.0 1,929,737 - RPSI07_RS07260 0.51 +0.5 1,929,780 + RPSI07_RS07260 0.58 -0.4 1,929,781 + RPSI07_RS07260 0.58 +1.4 1,929,782 - RPSI07_RS07260 0.59 +0.9 1,929,782 - RPSI07_RS07260 0.59 -0.4 1,929,782 - RPSI07_RS07260 0.59 -1.6 1,929,799 + RPSI07_RS07260 0.61 -0.7 1,929,800 - RPSI07_RS07260 0.62 -1.1 1,929,812 + RPSI07_RS07260 0.64 +0.9 1,929,812 + RPSI07_RS07260 0.64 -0.5 1,929,812 + RPSI07_RS07260 0.64 +0.9 1,929,812 + RPSI07_RS07260 0.64 -0.8 1,929,812 + RPSI07_RS07260 0.64 -0.1 1,929,812 + RPSI07_RS07260 0.64 -0.0 1,929,812 + RPSI07_RS07260 0.64 -0.1 1,929,813 - RPSI07_RS07260 0.64 -0.2 1,929,813 - RPSI07_RS07260 0.64 +1.9 1,929,813 - RPSI07_RS07260 0.64 -0.6 1,929,813 - RPSI07_RS07260 0.64 -1.4 1,929,814 + RPSI07_RS07260 0.64 -0.3 1,929,814 + RPSI07_RS07260 0.64 -0.0 1,929,814 + RPSI07_RS07260 0.64 +0.9 1,929,814 + RPSI07_RS07260 0.64 -1.4 1,929,814 + RPSI07_RS07260 0.64 +0.2 1,929,814 + RPSI07_RS07260 0.64 +0.0 1,929,814 + RPSI07_RS07260 0.64 -0.4 1,929,814 + RPSI07_RS07260 0.64 -0.6 1,929,815 - RPSI07_RS07260 0.64 +0.5 1,929,815 - RPSI07_RS07260 0.64 -0.5 1,929,815 - RPSI07_RS07260 0.64 -0.1 1,929,815 - RPSI07_RS07260 0.64 +0.6 1,929,815 - RPSI07_RS07260 0.64 -0.1 1,929,815 - RPSI07_RS07260 0.64 -0.2 1,929,815 - RPSI07_RS07260 0.64 -0.1 1,929,816 - RPSI07_RS07260 0.64 -0.4 1,929,816 - RPSI07_RS07260 0.64 -0.1 1,929,869 - RPSI07_RS07260 0.73 -1.0 1,930,063 + +0.5 1,930,063 + +0.4 1,930,063 + -0.7 1,930,063 + -0.4 1,930,063 + -0.2 1,930,064 - +0.9 1,930,083 + -1.1 1,930,083 + +0.1 1,930,084 - -1.3 1,930,084 - -1.1 1,930,094 + +2.5 1,930,095 - -2.6 1,930,318 + +0.0 1,930,318 + +1.9 1,930,318 + -0.4 1,930,318 + -0.8 1,930,319 - -0.9 1,930,319 - -0.9 1,930,319 - -0.5 1,930,610 + RPSI07_RS07265 0.44 -0.9 1,930,610 + RPSI07_RS07265 0.44 -1.0 1,930,610 + RPSI07_RS07265 0.44 +0.0 1,930,610 + RPSI07_RS07265 0.44 -0.5 1,930,610 + RPSI07_RS07265 0.44 -0.3 1,930,610 + RPSI07_RS07265 0.44 +0.9 1,930,610 + RPSI07_RS07265 0.44 -0.4 1,930,610 + RPSI07_RS07265 0.44 -0.9 1,930,611 - RPSI07_RS07265 0.44 -0.3 1,930,611 - RPSI07_RS07265 0.44 -0.3 1,930,611 - RPSI07_RS07265 0.44 +0.4 1,930,611 - RPSI07_RS07265 0.44 -1.1 1,930,611 - RPSI07_RS07265 0.44 -0.4 1,930,611 - RPSI07_RS07265 0.44 +0.6 1,930,611 - RPSI07_RS07265 0.44 -0.9 1,930,611 - RPSI07_RS07265 0.44 -0.8 1,930,611 - RPSI07_RS07265 0.44 -0.4 1,930,611 - RPSI07_RS07265 0.44 +0.5 1,930,611 - RPSI07_RS07265 0.44 -0.2 1,930,611 - RPSI07_RS07265 0.44 -0.6 1,930,611 - RPSI07_RS07265 0.44 -0.9 1,930,640 + RPSI07_RS07265 0.53 +0.3 1,930,640 + RPSI07_RS07265 0.53 +0.3 1,930,641 - RPSI07_RS07265 0.53 +1.2 1,930,641 - RPSI07_RS07265 0.53 +0.4 1,930,822 + +0.6 1,930,823 - -1.5 1,930,963 + +0.9 1,930,964 - +0.9 1,930,964 - +0.6 1,930,964 - -0.7 1,930,964 - -0.6 1,930,964 - -0.2 1,930,964 - -0.2 1,930,964 - +0.9 1,930,968 - -1.4 1,931,044 + RPSI07_RS07270 0.16 -0.0 1,931,044 + RPSI07_RS07270 0.16 +0.9 1,931,045 - RPSI07_RS07270 0.16 +0.9 1,931,045 - RPSI07_RS07270 0.16 +0.9 1,931,045 - RPSI07_RS07270 0.16 +0.4 1,931,045 - RPSI07_RS07270 0.16 +0.0 1,931,045 - RPSI07_RS07270 0.16 +0.3 1,931,123 + RPSI07_RS07270 0.31 -1.4 1,931,123 + RPSI07_RS07270 0.31 -0.6 1,931,123 + RPSI07_RS07270 0.31 -0.3 1,931,124 - RPSI07_RS07270 0.31 -0.2 1,931,124 - RPSI07_RS07270 0.31 +0.3 1,931,124 - RPSI07_RS07270 0.31 -0.3 1,931,124 - RPSI07_RS07270 0.31 +0.2 1,931,124 - RPSI07_RS07270 0.31 +0.9 1,931,124 - RPSI07_RS07270 0.31 -1.1 1,931,124 - RPSI07_RS07270 0.31 +0.1 1,931,125 + RPSI07_RS07270 0.31 -0.8 1,931,125 + RPSI07_RS07270 0.31 -0.4 1,931,125 + RPSI07_RS07270 0.31 +0.9 1,931,125 + RPSI07_RS07270 0.31 +0.4 1,931,125 + RPSI07_RS07270 0.31 +0.8 1,931,125 + RPSI07_RS07270 0.31 -0.6 1,931,125 + RPSI07_RS07270 0.31 -1.1 1,931,125 + RPSI07_RS07270 0.31 -1.6 1,931,125 + RPSI07_RS07270 0.31 -1.7 1,931,125 + RPSI07_RS07270 0.31 -1.0 1,931,125 + RPSI07_RS07270 0.31 +0.1 1,931,125 + RPSI07_RS07270 0.31 +0.5 1,931,125 - RPSI07_RS07270 0.31 -0.5 1,931,126 - RPSI07_RS07270 0.31 +1.1 1,931,126 - RPSI07_RS07270 0.31 +1.4 1,931,126 - RPSI07_RS07270 0.31 -0.1 1,931,126 - RPSI07_RS07270 0.31 +0.2 1,931,126 - RPSI07_RS07270 0.31 -0.4 1,931,270 - RPSI07_RS07270 0.59 +0.1 1,931,478 + +1.1 1,931,505 + +0.3 1,931,524 + +0.4 1,931,524 + -0.3 1,931,525 - +0.0 1,931,525 - -0.1 1,931,525 - -0.1 1,931,525 - +0.0 1,931,525 - +0.5 1,931,525 - -0.1 1,931,525 - +1.9 1,931,525 - -1.1 1,931,556 + +0.7 1,931,557 - -0.2 1,931,686 - RPSI07_RS07275 0.31 +1.5 1,931,754 + RPSI07_RS07275 0.51 +0.4 1,931,755 - RPSI07_RS07275 0.51 +1.1 1,931,757 + RPSI07_RS07275 0.52 +2.0 1,931,757 + RPSI07_RS07275 0.52 +1.5 1,931,757 + RPSI07_RS07275 0.52 -0.1 1,931,757 + RPSI07_RS07275 0.52 +1.4 1,931,757 + RPSI07_RS07275 0.52 +0.8 1,931,758 - RPSI07_RS07275 0.52 -0.1 1,931,758 - RPSI07_RS07275 0.52 +1.6
Or see this region's nucleotide sequence