Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS15795 and RPSI07_RS15800 are separated by 107 nucleotides RPSI07_RS15800 and RPSI07_RS15805 are separated by 104 nucleotides RPSI07_RS15805 and RPSI07_RS15810 are separated by 41 nucleotides
RPSI07_RS15795: RPSI07_RS15795 - oligopeptidase A, at 1,698,776 to 1,700,896
_RS15795
RPSI07_RS15800: RPSI07_RS15800 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD, at 1,701,004 to 1,701,873
_RS15800
RPSI07_RS15805: RPSI07_RS15805 - DNA-binding response regulator, at 1,701,978 to 1,702,610
_RS15805
RPSI07_RS15810: RPSI07_RS15810 - PAS domain-containing sensor histidine kinase, at 1,702,652 to 1,705,225
_RS15810
Position (kb)
1701
1702 Strain fitness (log2 ratio)
-1
0
1 at 1700.184 kb on + strand, within RPSI07_RS15795 at 1700.184 kb on + strand, within RPSI07_RS15795 at 1700.203 kb on - strand, within RPSI07_RS15795 at 1700.335 kb on - strand, within RPSI07_RS15795 at 1700.509 kb on - strand, within RPSI07_RS15795 at 1700.535 kb on + strand, within RPSI07_RS15795 at 1700.629 kb on + strand, within RPSI07_RS15795 at 1701.005 kb on + strand at 1701.006 kb on - strand at 1701.006 kb on - strand at 1702.019 kb on + strand at 1702.020 kb on - strand at 1702.501 kb on + strand, within RPSI07_RS15805 at 1702.626 kb on + strand at 1702.626 kb on + strand at 1702.626 kb on + strand at 1702.626 kb on + strand at 1702.627 kb on - strand at 1702.627 kb on - strand at 1702.627 kb on - strand at 1702.627 kb on - strand at 1702.688 kb on - strand at 1702.688 kb on - strand at 1702.742 kb on + strand at 1702.742 kb on + strand at 1702.742 kb on + strand at 1702.742 kb on + strand at 1702.742 kb on + strand at 1702.742 kb on + strand at 1702.742 kb on + strand at 1702.743 kb on - strand at 1702.743 kb on - strand at 1702.743 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,700,184 + RPSI07_RS15795 0.66 +0.7 1,700,184 + RPSI07_RS15795 0.66 +0.7 1,700,203 - RPSI07_RS15795 0.67 +0.4 1,700,335 - RPSI07_RS15795 0.74 -0.5 1,700,509 - RPSI07_RS15795 0.82 +0.7 1,700,535 + RPSI07_RS15795 0.83 +0.7 1,700,629 + RPSI07_RS15795 0.87 +0.7 1,701,005 + -0.5 1,701,006 - -1.6 1,701,006 - +0.0 1,702,019 + -1.4 1,702,020 - -0.3 1,702,501 + RPSI07_RS15805 0.83 -0.3 1,702,626 + +0.2 1,702,626 + +1.6 1,702,626 + +1.3 1,702,626 + -0.3 1,702,627 - -0.3 1,702,627 - -0.5 1,702,627 - -0.1 1,702,627 - -1.1 1,702,688 - -0.1 1,702,688 - +0.5 1,702,742 + -1.0 1,702,742 + -1.5 1,702,742 + -0.4 1,702,742 + -0.5 1,702,742 + -0.2 1,702,742 + -1.3 1,702,742 + -0.3 1,702,743 - -0.7 1,702,743 - -0.1 1,702,743 - -0.8
Or see this region's nucleotide sequence