Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS15720

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS15715 and RPSI07_RS15720 are separated by 66 nucleotidesRPSI07_RS15720 and RPSI07_RS15725 are separated by 67 nucleotidesRPSI07_RS15725 and RPSI07_RS15730 are separated by 24 nucleotidesRPSI07_RS15730 and RPSI07_RS15735 are separated by 58 nucleotides RPSI07_RS15715: RPSI07_RS15715 - phenylalanine--tRNA ligase subunit alpha, at 1,683,554 to 1,684,588 _RS15715 RPSI07_RS15720: RPSI07_RS15720 - phenylalanine--tRNA ligase subunit beta, at 1,684,655 to 1,687,084 _RS15720 RPSI07_RS15725: RPSI07_RS15725 - integration host factor subunit alpha, at 1,687,152 to 1,687,559 _RS15725 RPSI07_RS15730: RPSI07_RS15730 - MerR family transcriptional regulator, at 1,687,584 to 1,688,018 _RS15730 RPSI07_RS15735: RPSI07_RS15735 - tRNA-Pro, at 1,688,077 to 1,688,153 _RS15735 Position (kb) 1684 1685 1686 1687 1688Strain fitness (log2 ratio) -1 0 1at 1687.151 kb on + strandat 1687.557 kb on + strandat 1687.558 kb on - strandat 1687.558 kb on - strandat 1687.601 kb on + strandat 1687.601 kb on + strandat 1687.641 kb on + strand, within RPSI07_RS15730at 1687.755 kb on + strand, within RPSI07_RS15730at 1687.759 kb on - strandat 1687.759 kb on - strand, within RPSI07_RS15730at 1687.806 kb on + strand, within RPSI07_RS15730at 1687.807 kb on - strand, within RPSI07_RS15730at 1688.038 kb on - strandat 1688.061 kb on + strandat 1688.062 kb on - strandat 1688.063 kb on + strandat 1688.064 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,687,151 + -0.5
1,687,557 + +0.2
1,687,558 - -0.3
1,687,558 - +0.7
1,687,601 + -0.3
1,687,601 + -1.0
1,687,641 + RPSI07_RS15730 0.13 -0.9
1,687,755 + RPSI07_RS15730 0.39 +0.7
1,687,759 - +0.9
1,687,759 - RPSI07_RS15730 0.40 -0.8
1,687,806 + RPSI07_RS15730 0.51 -1.4
1,687,807 - RPSI07_RS15730 0.51 -1.6
1,688,038 - -1.6
1,688,061 + +0.1
1,688,062 - -0.3
1,688,063 + -0.6
1,688,064 - -1.7

Or see this region's nucleotide sequence