Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS15715 and RPSI07_RS15720 are separated by 66 nucleotides RPSI07_RS15720 and RPSI07_RS15725 are separated by 67 nucleotides RPSI07_RS15725 and RPSI07_RS15730 are separated by 24 nucleotides RPSI07_RS15730 and RPSI07_RS15735 are separated by 58 nucleotides
RPSI07_RS15715: RPSI07_RS15715 - phenylalanine--tRNA ligase subunit alpha, at 1,683,554 to 1,684,588
_RS15715
RPSI07_RS15720: RPSI07_RS15720 - phenylalanine--tRNA ligase subunit beta, at 1,684,655 to 1,687,084
_RS15720
RPSI07_RS15725: RPSI07_RS15725 - integration host factor subunit alpha, at 1,687,152 to 1,687,559
_RS15725
RPSI07_RS15730: RPSI07_RS15730 - MerR family transcriptional regulator, at 1,687,584 to 1,688,018
_RS15730
RPSI07_RS15735: RPSI07_RS15735 - tRNA-Pro, at 1,688,077 to 1,688,153
_RS15735
Position (kb)
1684
1685
1686
1687
1688 Strain fitness (log2 ratio)
-1
0
1 at 1687.151 kb on + strand at 1687.557 kb on + strand at 1687.558 kb on - strand at 1687.558 kb on - strand at 1687.601 kb on + strand at 1687.601 kb on + strand at 1687.641 kb on + strand, within RPSI07_RS15730 at 1687.755 kb on + strand, within RPSI07_RS15730 at 1687.759 kb on - strand at 1687.759 kb on - strand, within RPSI07_RS15730 at 1687.806 kb on + strand, within RPSI07_RS15730 at 1687.807 kb on - strand, within RPSI07_RS15730 at 1688.038 kb on - strand at 1688.061 kb on + strand at 1688.062 kb on - strand at 1688.063 kb on + strand at 1688.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,687,151 + -0.5 1,687,557 + +0.2 1,687,558 - -0.3 1,687,558 - +0.7 1,687,601 + -0.3 1,687,601 + -1.0 1,687,641 + RPSI07_RS15730 0.13 -0.9 1,687,755 + RPSI07_RS15730 0.39 +0.7 1,687,759 - +0.9 1,687,759 - RPSI07_RS15730 0.40 -0.8 1,687,806 + RPSI07_RS15730 0.51 -1.4 1,687,807 - RPSI07_RS15730 0.51 -1.6 1,688,038 - -1.6 1,688,061 + +0.1 1,688,062 - -0.3 1,688,063 + -0.6 1,688,064 - -1.7
Or see this region's nucleotide sequence