Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS15025 and RPSI07_RS15030 overlap by 4 nucleotides RPSI07_RS15030 and RPSI07_RS15035 are separated by 152 nucleotides
RPSI07_RS15025: RPSI07_RS15025 - NAD(P)/FAD-dependent oxidoreductase, at 1,539,060 to 1,540,580
_RS15025
RPSI07_RS15030: RPSI07_RS15030 - LysR family transcriptional regulator, at 1,540,577 to 1,541,551
_RS15030
RPSI07_RS15035: RPSI07_RS15035 - amino acid transporter, at 1,541,704 to 1,542,855
_RS15035
Position (kb)
1540
1541
1542 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1539.685 kb on + strand, within RPSI07_RS15025 at 1539.685 kb on + strand, within RPSI07_RS15025 at 1539.685 kb on + strand, within RPSI07_RS15025 at 1539.686 kb on - strand, within RPSI07_RS15025 at 1539.687 kb on - strand, within RPSI07_RS15025 at 1539.780 kb on + strand, within RPSI07_RS15025 at 1540.141 kb on + strand, within RPSI07_RS15025 at 1540.153 kb on + strand, within RPSI07_RS15025 at 1540.153 kb on + strand, within RPSI07_RS15025 at 1540.153 kb on + strand, within RPSI07_RS15025 at 1540.153 kb on + strand, within RPSI07_RS15025 at 1540.153 kb on + strand, within RPSI07_RS15025 at 1540.154 kb on - strand, within RPSI07_RS15025 at 1540.154 kb on - strand, within RPSI07_RS15025 at 1540.154 kb on - strand, within RPSI07_RS15025 at 1540.327 kb on + strand, within RPSI07_RS15025 at 1540.327 kb on + strand, within RPSI07_RS15025 at 1540.327 kb on + strand, within RPSI07_RS15025 at 1540.327 kb on + strand, within RPSI07_RS15025 at 1540.778 kb on + strand, within RPSI07_RS15030 at 1540.778 kb on + strand, within RPSI07_RS15030 at 1540.778 kb on + strand, within RPSI07_RS15030 at 1540.778 kb on + strand, within RPSI07_RS15030 at 1540.778 kb on + strand, within RPSI07_RS15030 at 1540.778 kb on + strand, within RPSI07_RS15030 at 1540.778 kb on + strand, within RPSI07_RS15030 at 1540.779 kb on - strand, within RPSI07_RS15030 at 1540.779 kb on - strand, within RPSI07_RS15030 at 1540.779 kb on - strand, within RPSI07_RS15030 at 1540.779 kb on - strand, within RPSI07_RS15030 at 1540.779 kb on - strand, within RPSI07_RS15030 at 1541.358 kb on + strand, within RPSI07_RS15030 at 1541.358 kb on + strand, within RPSI07_RS15030 at 1541.359 kb on - strand, within RPSI07_RS15030 at 1541.359 kb on - strand, within RPSI07_RS15030 at 1541.553 kb on + strand at 1541.554 kb on - strand at 1541.554 kb on - strand at 1541.607 kb on - strand at 1541.632 kb on + strand at 1541.633 kb on - strand at 1541.633 kb on - strand at 1541.664 kb on + strand at 1541.664 kb on + strand at 1541.665 kb on - strand at 1541.665 kb on - strand at 1541.705 kb on + strand at 1541.706 kb on - strand at 1541.706 kb on - strand at 1541.706 kb on - strand at 1541.903 kb on + strand, within RPSI07_RS15035 at 1541.922 kb on - strand, within RPSI07_RS15035 at 1542.328 kb on + strand, within RPSI07_RS15035 at 1542.386 kb on + strand, within RPSI07_RS15035 at 1542.504 kb on + strand, within RPSI07_RS15035
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,539,685 + RPSI07_RS15025 0.41 -2.0 1,539,685 + RPSI07_RS15025 0.41 +0.7 1,539,685 + RPSI07_RS15025 0.41 -0.3 1,539,686 - RPSI07_RS15025 0.41 -1.7 1,539,687 - RPSI07_RS15025 0.41 -0.6 1,539,780 + RPSI07_RS15025 0.47 -0.2 1,540,141 + RPSI07_RS15025 0.71 +0.2 1,540,153 + RPSI07_RS15025 0.72 -0.9 1,540,153 + RPSI07_RS15025 0.72 -0.1 1,540,153 + RPSI07_RS15025 0.72 -0.7 1,540,153 + RPSI07_RS15025 0.72 -1.6 1,540,153 + RPSI07_RS15025 0.72 +0.6 1,540,154 - RPSI07_RS15025 0.72 +0.9 1,540,154 - RPSI07_RS15025 0.72 -0.3 1,540,154 - RPSI07_RS15025 0.72 -0.3 1,540,327 + RPSI07_RS15025 0.83 -0.7 1,540,327 + RPSI07_RS15025 0.83 -0.2 1,540,327 + RPSI07_RS15025 0.83 -1.3 1,540,327 + RPSI07_RS15025 0.83 -0.3 1,540,778 + RPSI07_RS15030 0.21 +0.7 1,540,778 + RPSI07_RS15030 0.21 +3.3 1,540,778 + RPSI07_RS15030 0.21 -0.1 1,540,778 + RPSI07_RS15030 0.21 +0.1 1,540,778 + RPSI07_RS15030 0.21 +0.2 1,540,778 + RPSI07_RS15030 0.21 +0.2 1,540,778 + RPSI07_RS15030 0.21 +0.1 1,540,779 - RPSI07_RS15030 0.21 +0.2 1,540,779 - RPSI07_RS15030 0.21 -0.1 1,540,779 - RPSI07_RS15030 0.21 +0.2 1,540,779 - RPSI07_RS15030 0.21 +0.9 1,540,779 - RPSI07_RS15030 0.21 -0.5 1,541,358 + RPSI07_RS15030 0.80 +0.9 1,541,358 + RPSI07_RS15030 0.80 -0.4 1,541,359 - RPSI07_RS15030 0.80 +0.6 1,541,359 - RPSI07_RS15030 0.80 +0.3 1,541,553 + +0.3 1,541,554 - -1.4 1,541,554 - +0.2 1,541,607 - -0.3 1,541,632 + +0.0 1,541,633 - -0.3 1,541,633 - -0.4 1,541,664 + +0.4 1,541,664 + +0.1 1,541,665 - -1.0 1,541,665 - -3.3 1,541,705 + -0.4 1,541,706 - -0.5 1,541,706 - +2.1 1,541,706 - +0.7 1,541,903 + RPSI07_RS15035 0.17 +0.5 1,541,922 - RPSI07_RS15035 0.19 -0.8 1,542,328 + RPSI07_RS15035 0.54 -0.2 1,542,386 + RPSI07_RS15035 0.59 +0.0 1,542,504 + RPSI07_RS15035 0.69 -0.2
Or see this region's nucleotide sequence