Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21875 and RPSI07_RS21880 are separated by 105 nucleotides RPSI07_RS21880 and RPSI07_RS21885 are separated by 58 nucleotides RPSI07_RS21885 and RPSI07_RS21890 are separated by 101 nucleotides RPSI07_RS21890 and RPSI07_RS21895 are separated by 561 nucleotides
RPSI07_RS21875: RPSI07_RS21875 - FAA hydrolase family protein, at 3,069,040 to 3,069,888
_RS21875
RPSI07_RS21880: RPSI07_RS21880 - glycosyltransferase family 2 protein, at 3,069,994 to 3,070,767
_RS21880
RPSI07_RS21885: RPSI07_RS21885 - 3-oxoacyl-ACP reductase FabG, at 3,070,826 to 3,071,542
_RS21885
RPSI07_RS21890: RPSI07_RS21890 - acyl carrier protein, at 3,071,644 to 3,071,928
_RS21890
RPSI07_RS21895: RPSI07_RS21895 - membrane protein, at 3,072,490 to 3,073,146
_RS21895
Position (kb)
3070
3071
3072 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3069.995 kb on + strand at 3070.100 kb on + strand, within RPSI07_RS21880 at 3070.101 kb on - strand, within RPSI07_RS21880 at 3070.101 kb on - strand, within RPSI07_RS21880 at 3070.274 kb on + strand, within RPSI07_RS21880 at 3070.718 kb on + strand at 3070.718 kb on + strand at 3070.718 kb on + strand at 3070.718 kb on + strand at 3070.718 kb on + strand at 3070.719 kb on - strand at 3070.719 kb on - strand at 3070.719 kb on - strand at 3070.719 kb on - strand at 3070.719 kb on - strand at 3070.719 kb on - strand at 3070.869 kb on + strand at 3070.869 kb on + strand at 3070.869 kb on + strand at 3070.869 kb on + strand at 3070.869 kb on + strand at 3070.869 kb on + strand at 3070.870 kb on - strand at 3070.870 kb on - strand at 3070.893 kb on + strand at 3070.943 kb on + strand, within RPSI07_RS21885 at 3071.097 kb on + strand, within RPSI07_RS21885 at 3071.097 kb on + strand, within RPSI07_RS21885 at 3071.097 kb on + strand, within RPSI07_RS21885 at 3071.097 kb on + strand, within RPSI07_RS21885 at 3071.098 kb on - strand, within RPSI07_RS21885 at 3071.098 kb on - strand, within RPSI07_RS21885 at 3071.098 kb on - strand, within RPSI07_RS21885 at 3071.143 kb on + strand, within RPSI07_RS21885 at 3071.144 kb on - strand, within RPSI07_RS21885 at 3071.144 kb on - strand, within RPSI07_RS21885 at 3071.669 kb on + strand at 3071.669 kb on + strand at 3071.670 kb on - strand at 3071.670 kb on - strand at 3071.807 kb on + strand, within RPSI07_RS21890 at 3071.808 kb on - strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.809 kb on + strand, within RPSI07_RS21890 at 3071.810 kb on - strand, within RPSI07_RS21890 at 3071.810 kb on - strand, within RPSI07_RS21890 at 3071.810 kb on - strand, within RPSI07_RS21890 at 3071.810 kb on - strand, within RPSI07_RS21890 at 3071.810 kb on - strand at 3071.810 kb on - strand, within RPSI07_RS21890 at 3071.810 kb on - strand, within RPSI07_RS21890 at 3071.810 kb on - strand, within RPSI07_RS21890 at 3071.810 kb on - strand, within RPSI07_RS21890 at 3071.810 kb on - strand, within RPSI07_RS21890 at 3071.810 kb on - strand, within RPSI07_RS21890 at 3071.810 kb on - strand, within RPSI07_RS21890 at 3071.810 kb on - strand, within RPSI07_RS21890 at 3071.810 kb on - strand, within RPSI07_RS21890 at 3071.810 kb on - strand, within RPSI07_RS21890 at 3071.810 kb on - strand, within RPSI07_RS21890 at 3071.844 kb on + strand, within RPSI07_RS21890 at 3071.845 kb on - strand, within RPSI07_RS21890 at 3071.845 kb on - strand, within RPSI07_RS21890 at 3071.845 kb on - strand, within RPSI07_RS21890 at 3071.845 kb on - strand, within RPSI07_RS21890 at 3072.015 kb on - strand at 3072.027 kb on + strand at 3072.027 kb on + strand at 3072.027 kb on + strand at 3072.027 kb on + strand at 3072.044 kb on - strand at 3072.064 kb on + strand at 3072.064 kb on + strand at 3072.088 kb on + strand at 3072.088 kb on + strand at 3072.088 kb on + strand at 3072.088 kb on + strand at 3072.088 kb on + strand at 3072.089 kb on - strand at 3072.089 kb on - strand at 3072.118 kb on + strand at 3072.118 kb on + strand at 3072.118 kb on + strand at 3072.119 kb on - strand at 3072.119 kb on - strand at 3072.119 kb on - strand at 3072.243 kb on + strand at 3072.244 kb on - strand at 3072.244 kb on - strand at 3072.244 kb on - strand at 3072.299 kb on + strand at 3072.299 kb on + strand at 3072.300 kb on - strand at 3072.300 kb on - strand at 3072.300 kb on - strand at 3072.300 kb on - strand at 3072.300 kb on - strand at 3072.300 kb on - strand at 3072.300 kb on - strand at 3072.361 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 3,069,995 + +0.5 3,070,100 + RPSI07_RS21880 0.14 -1.2 3,070,101 - RPSI07_RS21880 0.14 -1.4 3,070,101 - RPSI07_RS21880 0.14 -0.2 3,070,274 + RPSI07_RS21880 0.36 +0.8 3,070,718 + -0.5 3,070,718 + +0.8 3,070,718 + -0.5 3,070,718 + -0.5 3,070,718 + -0.3 3,070,719 - -0.4 3,070,719 - +0.4 3,070,719 - -0.0 3,070,719 - +0.8 3,070,719 - +0.3 3,070,719 - -1.8 3,070,869 + +0.2 3,070,869 + +0.1 3,070,869 + +0.0 3,070,869 + -0.7 3,070,869 + -0.6 3,070,869 + -0.5 3,070,870 - -1.0 3,070,870 - -0.1 3,070,893 + -1.3 3,070,943 + RPSI07_RS21885 0.16 +0.8 3,071,097 + RPSI07_RS21885 0.38 +1.8 3,071,097 + RPSI07_RS21885 0.38 -0.1 3,071,097 + RPSI07_RS21885 0.38 -1.2 3,071,097 + RPSI07_RS21885 0.38 +1.6 3,071,098 - RPSI07_RS21885 0.38 -1.0 3,071,098 - RPSI07_RS21885 0.38 -1.4 3,071,098 - RPSI07_RS21885 0.38 +0.3 3,071,143 + RPSI07_RS21885 0.44 +0.2 3,071,144 - RPSI07_RS21885 0.44 -0.5 3,071,144 - RPSI07_RS21885 0.44 +0.6 3,071,669 + +0.2 3,071,669 + -0.3 3,071,670 - -1.5 3,071,670 - -0.1 3,071,807 + RPSI07_RS21890 0.57 +0.0 3,071,808 - RPSI07_RS21890 0.58 +0.5 3,071,809 + RPSI07_RS21890 0.58 +0.0 3,071,809 + RPSI07_RS21890 0.58 -0.5 3,071,809 + RPSI07_RS21890 0.58 +1.8 3,071,809 + RPSI07_RS21890 0.58 +0.4 3,071,809 + RPSI07_RS21890 0.58 +0.2 3,071,809 + RPSI07_RS21890 0.58 -1.0 3,071,809 + RPSI07_RS21890 0.58 -0.4 3,071,809 + RPSI07_RS21890 0.58 -0.4 3,071,809 + RPSI07_RS21890 0.58 -0.2 3,071,809 + RPSI07_RS21890 0.58 -1.2 3,071,809 + RPSI07_RS21890 0.58 +0.0 3,071,809 + RPSI07_RS21890 0.58 +0.2 3,071,809 + RPSI07_RS21890 0.58 -0.2 3,071,809 + RPSI07_RS21890 0.58 -0.3 3,071,809 + RPSI07_RS21890 0.58 -0.6 3,071,809 + RPSI07_RS21890 0.58 -0.1 3,071,809 + RPSI07_RS21890 0.58 +0.2 3,071,810 - RPSI07_RS21890 0.58 -0.5 3,071,810 - RPSI07_RS21890 0.58 -0.2 3,071,810 - RPSI07_RS21890 0.58 -0.2 3,071,810 - RPSI07_RS21890 0.58 -0.8 3,071,810 - +0.8 3,071,810 - RPSI07_RS21890 0.58 +0.2 3,071,810 - RPSI07_RS21890 0.58 +0.8 3,071,810 - RPSI07_RS21890 0.58 -0.3 3,071,810 - RPSI07_RS21890 0.58 +0.8 3,071,810 - RPSI07_RS21890 0.58 -1.5 3,071,810 - RPSI07_RS21890 0.58 +0.3 3,071,810 - RPSI07_RS21890 0.58 +0.2 3,071,810 - RPSI07_RS21890 0.58 -0.6 3,071,810 - RPSI07_RS21890 0.58 +0.4 3,071,810 - RPSI07_RS21890 0.58 -0.8 3,071,810 - RPSI07_RS21890 0.58 -1.4 3,071,844 + RPSI07_RS21890 0.70 -0.2 3,071,845 - RPSI07_RS21890 0.71 -2.7 3,071,845 - RPSI07_RS21890 0.71 +2.4 3,071,845 - RPSI07_RS21890 0.71 -0.5 3,071,845 - RPSI07_RS21890 0.71 +1.8 3,072,015 - +0.6 3,072,027 + +0.5 3,072,027 + -0.1 3,072,027 + -0.4 3,072,027 + +0.7 3,072,044 - -0.2 3,072,064 + -0.0 3,072,064 + +0.1 3,072,088 + +0.2 3,072,088 + -1.1 3,072,088 + -0.1 3,072,088 + -0.8 3,072,088 + -0.0 3,072,089 - +0.8 3,072,089 - -1.8 3,072,118 + -0.5 3,072,118 + -0.3 3,072,118 + -0.6 3,072,119 - +0.3 3,072,119 - +0.8 3,072,119 - +0.8 3,072,243 + +0.3 3,072,244 - -1.0 3,072,244 - -0.3 3,072,244 - -0.5 3,072,299 + -1.2 3,072,299 + -0.1 3,072,300 - -0.7 3,072,300 - -0.5 3,072,300 - +0.0 3,072,300 - +0.8 3,072,300 - +0.2 3,072,300 - +0.0 3,072,300 - -0.5 3,072,361 - -0.8
Or see this region's nucleotide sequence