Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Penicillin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS20255 and RPSI07_RS20260 are separated by 108 nucleotides RPSI07_RS20260 and RPSI07_RS20265 are separated by 74 nucleotides RPSI07_RS20265 and RPSI07_RS20270 are separated by 252 nucleotides
RPSI07_RS20255: RPSI07_RS20255 - acyl-CoA dehydrogenase, at 2,725,826 to 2,726,998
_RS20255
RPSI07_RS20260: RPSI07_RS20260 - nucleoside 2-deoxyribosyltransferase, at 2,727,107 to 2,727,592
_RS20260
RPSI07_RS20265: RPSI07_RS20265 - DNA/RNA non-specific endonuclease, at 2,727,667 to 2,728,494
_RS20265
RPSI07_RS20270: RPSI07_RS20270 - 3-deoxy-7-phosphoheptulonate synthase, at 2,728,747 to 2,729,865
_RS20270
Position (kb)
2727
2728
2729 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2726.715 kb on + strand, within RPSI07_RS20255 at 2726.715 kb on + strand, within RPSI07_RS20255 at 2726.838 kb on + strand, within RPSI07_RS20255 at 2726.838 kb on + strand, within RPSI07_RS20255 at 2726.839 kb on - strand, within RPSI07_RS20255 at 2727.036 kb on + strand at 2727.036 kb on + strand at 2727.037 kb on - strand at 2727.037 kb on - strand at 2727.038 kb on + strand at 2727.038 kb on + strand at 2727.038 kb on + strand at 2727.038 kb on + strand at 2727.038 kb on + strand at 2727.039 kb on - strand at 2727.039 kb on - strand at 2727.039 kb on - strand at 2727.039 kb on - strand at 2727.098 kb on + strand at 2727.114 kb on + strand at 2727.285 kb on + strand, within RPSI07_RS20260 at 2727.285 kb on + strand, within RPSI07_RS20260 at 2727.285 kb on + strand, within RPSI07_RS20260 at 2727.286 kb on - strand, within RPSI07_RS20260 at 2727.286 kb on - strand, within RPSI07_RS20260 at 2727.286 kb on - strand, within RPSI07_RS20260 at 2727.286 kb on - strand, within RPSI07_RS20260 at 2727.286 kb on - strand, within RPSI07_RS20260 at 2727.319 kb on - strand, within RPSI07_RS20260 at 2727.319 kb on - strand, within RPSI07_RS20260 at 2727.423 kb on + strand, within RPSI07_RS20260 at 2727.484 kb on - strand, within RPSI07_RS20260 at 2727.576 kb on + strand at 2727.576 kb on + strand at 2727.577 kb on - strand at 2727.692 kb on - strand at 2727.692 kb on - strand at 2727.692 kb on - strand at 2727.692 kb on - strand at 2727.934 kb on + strand, within RPSI07_RS20265 at 2727.934 kb on + strand, within RPSI07_RS20265 at 2727.935 kb on - strand, within RPSI07_RS20265 at 2727.935 kb on - strand, within RPSI07_RS20265 at 2727.961 kb on + strand, within RPSI07_RS20265 at 2728.078 kb on + strand, within RPSI07_RS20265 at 2728.079 kb on - strand, within RPSI07_RS20265 at 2728.079 kb on - strand, within RPSI07_RS20265 at 2728.079 kb on - strand, within RPSI07_RS20265 at 2728.237 kb on + strand, within RPSI07_RS20265 at 2728.264 kb on + strand, within RPSI07_RS20265 at 2728.264 kb on + strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.336 kb on + strand, within RPSI07_RS20265 at 2728.337 kb on - strand, within RPSI07_RS20265 at 2728.342 kb on + strand, within RPSI07_RS20265 at 2728.342 kb on + strand, within RPSI07_RS20265 at 2728.416 kb on - strand at 2729.081 kb on + strand, within RPSI07_RS20270 at 2729.081 kb on + strand, within RPSI07_RS20270 at 2729.081 kb on + strand, within RPSI07_RS20270 at 2729.082 kb on - strand, within RPSI07_RS20270 at 2729.351 kb on + strand, within RPSI07_RS20270 at 2729.351 kb on + strand, within RPSI07_RS20270 at 2729.486 kb on + strand, within RPSI07_RS20270
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Penicillin remove 2,726,715 + RPSI07_RS20255 0.76 +0.6 2,726,715 + RPSI07_RS20255 0.76 +1.7 2,726,838 + RPSI07_RS20255 0.86 -0.2 2,726,838 + RPSI07_RS20255 0.86 +0.7 2,726,839 - RPSI07_RS20255 0.86 -1.1 2,727,036 + -0.4 2,727,036 + +0.7 2,727,037 - -0.3 2,727,037 - +0.9 2,727,038 + -0.7 2,727,038 + +0.1 2,727,038 + -0.3 2,727,038 + +0.0 2,727,038 + -0.1 2,727,039 - -1.3 2,727,039 - +0.5 2,727,039 - -0.3 2,727,039 - -0.3 2,727,098 + -0.5 2,727,114 + +0.0 2,727,285 + RPSI07_RS20260 0.37 -0.3 2,727,285 + RPSI07_RS20260 0.37 +0.3 2,727,285 + RPSI07_RS20260 0.37 -0.1 2,727,286 - RPSI07_RS20260 0.37 +0.4 2,727,286 - RPSI07_RS20260 0.37 -0.3 2,727,286 - RPSI07_RS20260 0.37 +0.1 2,727,286 - RPSI07_RS20260 0.37 +0.3 2,727,286 - RPSI07_RS20260 0.37 +0.7 2,727,319 - RPSI07_RS20260 0.44 +0.1 2,727,319 - RPSI07_RS20260 0.44 +1.7 2,727,423 + RPSI07_RS20260 0.65 +0.6 2,727,484 - RPSI07_RS20260 0.78 -1.6 2,727,576 + +0.7 2,727,576 + +0.3 2,727,577 - +0.7 2,727,692 - -1.0 2,727,692 - -0.7 2,727,692 - +0.9 2,727,692 - +0.4 2,727,934 + RPSI07_RS20265 0.32 -0.7 2,727,934 + RPSI07_RS20265 0.32 +1.8 2,727,935 - RPSI07_RS20265 0.32 -0.0 2,727,935 - RPSI07_RS20265 0.32 +0.2 2,727,961 + RPSI07_RS20265 0.36 -0.3 2,728,078 + RPSI07_RS20265 0.50 +0.7 2,728,079 - RPSI07_RS20265 0.50 -1.0 2,728,079 - RPSI07_RS20265 0.50 +0.5 2,728,079 - RPSI07_RS20265 0.50 +0.7 2,728,237 + RPSI07_RS20265 0.69 -0.1 2,728,264 + RPSI07_RS20265 0.72 -0.3 2,728,264 + RPSI07_RS20265 0.72 -0.0 2,728,265 - RPSI07_RS20265 0.72 -0.9 2,728,265 - RPSI07_RS20265 0.72 -0.6 2,728,265 - RPSI07_RS20265 0.72 -0.4 2,728,265 - RPSI07_RS20265 0.72 -0.5 2,728,265 - RPSI07_RS20265 0.72 -0.5 2,728,265 - RPSI07_RS20265 0.72 -0.1 2,728,265 - RPSI07_RS20265 0.72 -1.3 2,728,265 - RPSI07_RS20265 0.72 -0.3 2,728,265 - RPSI07_RS20265 0.72 -0.5 2,728,265 - RPSI07_RS20265 0.72 +0.0 2,728,336 + RPSI07_RS20265 0.81 +0.5 2,728,337 - RPSI07_RS20265 0.81 +0.0 2,728,342 + RPSI07_RS20265 0.82 +0.1 2,728,342 + RPSI07_RS20265 0.82 -0.5 2,728,416 - +0.1 2,729,081 + RPSI07_RS20270 0.30 +0.5 2,729,081 + RPSI07_RS20270 0.30 -2.0 2,729,081 + RPSI07_RS20270 0.30 -0.5 2,729,082 - RPSI07_RS20270 0.30 -0.3 2,729,351 + RPSI07_RS20270 0.54 +0.3 2,729,351 + RPSI07_RS20270 0.54 -0.3 2,729,486 + RPSI07_RS20270 0.66 +0.5
Or see this region's nucleotide sequence