Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS22325

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS22320 and RPSI07_RS22325 are separated by 3 nucleotidesRPSI07_RS22325 and RPSI07_RS22330 are separated by 52 nucleotidesRPSI07_RS22330 and RPSI07_RS22335 are separated by 55 nucleotides RPSI07_RS22320: RPSI07_RS22320 - heme A synthase, at 3,171,630 to 3,172,721 _RS22320 RPSI07_RS22325: RPSI07_RS22325 - cytochrome c oxidase subunit I, at 3,172,725 to 3,173,372 _RS22325 RPSI07_RS22330: RPSI07_RS22330 - SURF1 family protein, at 3,173,425 to 3,174,195 _RS22330 RPSI07_RS22335: RPSI07_RS22335 - twin transmembrane helix small protein, at 3,174,251 to 3,174,460 _RS22335 Position (kb) 3172 3173 3174Strain fitness (log2 ratio) -2 -1 0 1at 3172.415 kb on - strand, within RPSI07_RS22320at 3172.525 kb on + strand, within RPSI07_RS22320at 3172.526 kb on - strand, within RPSI07_RS22320at 3172.666 kb on + strandat 3172.667 kb on - strandat 3172.667 kb on - strandat 3172.667 kb on - strandat 3172.667 kb on - strandat 3172.667 kb on - strandat 3172.667 kb on - strandat 3172.667 kb on - strandat 3172.834 kb on + strand, within RPSI07_RS22325at 3172.918 kb on + strand, within RPSI07_RS22325at 3173.227 kb on + strand, within RPSI07_RS22325at 3173.228 kb on - strand, within RPSI07_RS22325at 3173.228 kb on - strand, within RPSI07_RS22325at 3173.230 kb on + strand, within RPSI07_RS22325at 3173.385 kb on - strandat 3173.385 kb on - strandat 3173.390 kb on + strandat 3173.391 kb on - strandat 3173.475 kb on - strandat 3173.665 kb on - strand, within RPSI07_RS22330at 3173.673 kb on + strand, within RPSI07_RS22330at 3173.674 kb on - strand, within RPSI07_RS22330at 3173.674 kb on - strand, within RPSI07_RS22330at 3173.719 kb on - strand, within RPSI07_RS22330at 3173.783 kb on + strand, within RPSI07_RS22330at 3173.784 kb on - strand, within RPSI07_RS22330at 3173.919 kb on + strand, within RPSI07_RS22330at 3173.920 kb on - strand, within RPSI07_RS22330at 3173.945 kb on + strand, within RPSI07_RS22330at 3173.946 kb on + strand, within RPSI07_RS22330at 3173.988 kb on - strand, within RPSI07_RS22330at 3173.988 kb on - strand, within RPSI07_RS22330at 3173.988 kb on - strand, within RPSI07_RS22330at 3174.175 kb on + strandat 3174.175 kb on + strandat 3174.214 kb on + strandat 3174.214 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
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3,172,415 - RPSI07_RS22320 0.72 +0.6
3,172,525 + RPSI07_RS22320 0.82 +1.6
3,172,526 - RPSI07_RS22320 0.82 -1.3
3,172,666 + -1.5
3,172,667 - -1.5
3,172,667 - +0.1
3,172,667 - +0.4
3,172,667 - -0.3
3,172,667 - +0.7
3,172,667 - +0.1
3,172,667 - -0.1
3,172,834 + RPSI07_RS22325 0.17 -0.5
3,172,918 + RPSI07_RS22325 0.30 +0.1
3,173,227 + RPSI07_RS22325 0.77 +1.1
3,173,228 - RPSI07_RS22325 0.78 -0.9
3,173,228 - RPSI07_RS22325 0.78 -1.2
3,173,230 + RPSI07_RS22325 0.78 -1.1
3,173,385 - -0.3
3,173,385 - -1.3
3,173,390 + +0.6
3,173,391 - -0.2
3,173,475 - +1.1
3,173,665 - RPSI07_RS22330 0.31 -2.2
3,173,673 + RPSI07_RS22330 0.32 +1.1
3,173,674 - RPSI07_RS22330 0.32 -0.8
3,173,674 - RPSI07_RS22330 0.32 -0.4
3,173,719 - RPSI07_RS22330 0.38 -0.9
3,173,783 + RPSI07_RS22330 0.46 +0.1
3,173,784 - RPSI07_RS22330 0.47 -0.9
3,173,919 + RPSI07_RS22330 0.64 -0.4
3,173,920 - RPSI07_RS22330 0.64 -0.3
3,173,945 + RPSI07_RS22330 0.67 -0.3
3,173,946 + RPSI07_RS22330 0.68 -1.0
3,173,988 - RPSI07_RS22330 0.73 -1.9
3,173,988 - RPSI07_RS22330 0.73 -0.7
3,173,988 - RPSI07_RS22330 0.73 -0.9
3,174,175 + +1.3
3,174,175 + -0.6
3,174,214 + -0.3
3,174,214 + +0.6

Or see this region's nucleotide sequence