Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS22320 and RPSI07_RS22325 are separated by 3 nucleotides RPSI07_RS22325 and RPSI07_RS22330 are separated by 52 nucleotides RPSI07_RS22330 and RPSI07_RS22335 are separated by 55 nucleotides
RPSI07_RS22320: RPSI07_RS22320 - heme A synthase, at 3,171,630 to 3,172,721
_RS22320
RPSI07_RS22325: RPSI07_RS22325 - cytochrome c oxidase subunit I, at 3,172,725 to 3,173,372
_RS22325
RPSI07_RS22330: RPSI07_RS22330 - SURF1 family protein, at 3,173,425 to 3,174,195
_RS22330
RPSI07_RS22335: RPSI07_RS22335 - twin transmembrane helix small protein, at 3,174,251 to 3,174,460
_RS22335
Position (kb)
3172
3173
3174 Strain fitness (log2 ratio)
-2
-1
0
1 at 3172.415 kb on - strand, within RPSI07_RS22320 at 3172.525 kb on + strand, within RPSI07_RS22320 at 3172.526 kb on - strand, within RPSI07_RS22320 at 3172.666 kb on + strand at 3172.667 kb on - strand at 3172.667 kb on - strand at 3172.667 kb on - strand at 3172.667 kb on - strand at 3172.667 kb on - strand at 3172.667 kb on - strand at 3172.667 kb on - strand at 3172.834 kb on + strand, within RPSI07_RS22325 at 3172.918 kb on + strand, within RPSI07_RS22325 at 3173.227 kb on + strand, within RPSI07_RS22325 at 3173.228 kb on - strand, within RPSI07_RS22325 at 3173.228 kb on - strand, within RPSI07_RS22325 at 3173.230 kb on + strand, within RPSI07_RS22325 at 3173.385 kb on - strand at 3173.385 kb on - strand at 3173.390 kb on + strand at 3173.391 kb on - strand at 3173.475 kb on - strand at 3173.665 kb on - strand, within RPSI07_RS22330 at 3173.673 kb on + strand, within RPSI07_RS22330 at 3173.674 kb on - strand, within RPSI07_RS22330 at 3173.674 kb on - strand, within RPSI07_RS22330 at 3173.719 kb on - strand, within RPSI07_RS22330 at 3173.783 kb on + strand, within RPSI07_RS22330 at 3173.784 kb on - strand, within RPSI07_RS22330 at 3173.919 kb on + strand, within RPSI07_RS22330 at 3173.920 kb on - strand, within RPSI07_RS22330 at 3173.945 kb on + strand, within RPSI07_RS22330 at 3173.946 kb on + strand, within RPSI07_RS22330 at 3173.988 kb on - strand, within RPSI07_RS22330 at 3173.988 kb on - strand, within RPSI07_RS22330 at 3173.988 kb on - strand, within RPSI07_RS22330 at 3174.175 kb on + strand at 3174.175 kb on + strand at 3174.214 kb on + strand at 3174.214 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 3,172,415 - RPSI07_RS22320 0.72 +0.6 3,172,525 + RPSI07_RS22320 0.82 +1.6 3,172,526 - RPSI07_RS22320 0.82 -1.3 3,172,666 + -1.5 3,172,667 - -1.5 3,172,667 - +0.1 3,172,667 - +0.4 3,172,667 - -0.3 3,172,667 - +0.7 3,172,667 - +0.1 3,172,667 - -0.1 3,172,834 + RPSI07_RS22325 0.17 -0.5 3,172,918 + RPSI07_RS22325 0.30 +0.1 3,173,227 + RPSI07_RS22325 0.77 +1.1 3,173,228 - RPSI07_RS22325 0.78 -0.9 3,173,228 - RPSI07_RS22325 0.78 -1.2 3,173,230 + RPSI07_RS22325 0.78 -1.1 3,173,385 - -0.3 3,173,385 - -1.3 3,173,390 + +0.6 3,173,391 - -0.2 3,173,475 - +1.1 3,173,665 - RPSI07_RS22330 0.31 -2.2 3,173,673 + RPSI07_RS22330 0.32 +1.1 3,173,674 - RPSI07_RS22330 0.32 -0.8 3,173,674 - RPSI07_RS22330 0.32 -0.4 3,173,719 - RPSI07_RS22330 0.38 -0.9 3,173,783 + RPSI07_RS22330 0.46 +0.1 3,173,784 - RPSI07_RS22330 0.47 -0.9 3,173,919 + RPSI07_RS22330 0.64 -0.4 3,173,920 - RPSI07_RS22330 0.64 -0.3 3,173,945 + RPSI07_RS22330 0.67 -0.3 3,173,946 + RPSI07_RS22330 0.68 -1.0 3,173,988 - RPSI07_RS22330 0.73 -1.9 3,173,988 - RPSI07_RS22330 0.73 -0.7 3,173,988 - RPSI07_RS22330 0.73 -0.9 3,174,175 + +1.3 3,174,175 + -0.6 3,174,214 + -0.3 3,174,214 + +0.6
Or see this region's nucleotide sequence