Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21620 and RPSI07_RS21625 overlap by 4 nucleotides RPSI07_RS21625 and RPSI07_RS21630 are separated by 36 nucleotides
RPSI07_RS21620: RPSI07_RS21620 - DUF3025 domain-containing protein, at 3,009,493 to 3,010,314
_RS21620
RPSI07_RS21625: RPSI07_RS21625 - dihydroorotase, at 3,010,311 to 3,011,345
_RS21625
RPSI07_RS21630: RPSI07_RS21630 - glycerate kinase, at 3,011,382 to 3,012,680
_RS21630
Position (kb)
3010
3011
3012 Strain fitness (log2 ratio)
-2
-1
0
1 at 3009.494 kb on + strand at 3009.495 kb on - strand at 3009.495 kb on - strand at 3009.557 kb on + strand at 3009.557 kb on + strand at 3009.558 kb on - strand at 3009.558 kb on - strand at 3009.558 kb on - strand at 3009.558 kb on - strand at 3009.746 kb on + strand, within RPSI07_RS21620 at 3009.746 kb on + strand, within RPSI07_RS21620 at 3010.017 kb on - strand at 3010.097 kb on + strand, within RPSI07_RS21620 at 3010.097 kb on + strand, within RPSI07_RS21620 at 3010.098 kb on - strand, within RPSI07_RS21620 at 3010.098 kb on - strand, within RPSI07_RS21620 at 3011.471 kb on - strand at 3011.471 kb on - strand at 3011.605 kb on + strand, within RPSI07_RS21630 at 3011.605 kb on + strand, within RPSI07_RS21630 at 3011.785 kb on + strand, within RPSI07_RS21630 at 3011.785 kb on + strand, within RPSI07_RS21630 at 3011.785 kb on + strand, within RPSI07_RS21630 at 3011.786 kb on - strand, within RPSI07_RS21630 at 3011.786 kb on - strand, within RPSI07_RS21630 at 3011.786 kb on - strand, within RPSI07_RS21630 at 3011.786 kb on - strand, within RPSI07_RS21630 at 3011.822 kb on + strand, within RPSI07_RS21630 at 3011.822 kb on + strand, within RPSI07_RS21630 at 3011.823 kb on - strand, within RPSI07_RS21630 at 3011.823 kb on - strand, within RPSI07_RS21630 at 3012.050 kb on - strand, within RPSI07_RS21630
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol remove 3,009,494 + -0.9 3,009,495 - -0.5 3,009,495 - -0.5 3,009,557 + -0.2 3,009,557 + -0.1 3,009,558 - -0.2 3,009,558 - -0.7 3,009,558 - +0.3 3,009,558 - -0.1 3,009,746 + RPSI07_RS21620 0.31 -0.4 3,009,746 + RPSI07_RS21620 0.31 -1.4 3,010,017 - -0.3 3,010,097 + RPSI07_RS21620 0.73 -0.6 3,010,097 + RPSI07_RS21620 0.73 -0.6 3,010,098 - RPSI07_RS21620 0.74 +0.2 3,010,098 - RPSI07_RS21620 0.74 +1.4 3,011,471 - -1.0 3,011,471 - +0.0 3,011,605 + RPSI07_RS21630 0.17 -1.8 3,011,605 + RPSI07_RS21630 0.17 -0.6 3,011,785 + RPSI07_RS21630 0.31 -0.1 3,011,785 + RPSI07_RS21630 0.31 -0.7 3,011,785 + RPSI07_RS21630 0.31 +0.0 3,011,786 - RPSI07_RS21630 0.31 -0.6 3,011,786 - RPSI07_RS21630 0.31 +1.0 3,011,786 - RPSI07_RS21630 0.31 +0.1 3,011,786 - RPSI07_RS21630 0.31 -0.2 3,011,822 + RPSI07_RS21630 0.34 +0.2 3,011,822 + RPSI07_RS21630 0.34 +0.7 3,011,823 - RPSI07_RS21630 0.34 +0.0 3,011,823 - RPSI07_RS21630 0.34 +0.0 3,012,050 - RPSI07_RS21630 0.51 -0.1
Or see this region's nucleotide sequence