Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10510 and RPSI07_RS10515 are separated by 26 nucleotides RPSI07_RS10515 and RPSI07_RS10520 are separated by 145 nucleotides RPSI07_RS10520 and RPSI07_RS10525 are separated by 58 nucleotides
RPSI07_RS10510: RPSI07_RS10510 - cytochrome b, at 559,554 to 560,957
_RS10510
RPSI07_RS10515: RPSI07_RS10515 - cytochrome c, at 560,984 to 561,730
_RS10515
RPSI07_RS10520: RPSI07_RS10520 - transcription modulator protein, at 561,876 to 562,487
_RS10520
RPSI07_RS10525: RPSI07_RS10525 - ClpXP protease specificity-enhancing factor, at 562,546 to 563,043
_RS10525
Position (kb) 560
561
562 Strain fitness (log2 ratio) -1
0
1
2 at 560.034 kb on + strand, within RPSI07_RS10510 at 560.313 kb on + strand, within RPSI07_RS10510 at 560.317 kb on - strand, within RPSI07_RS10510 at 560.456 kb on + strand, within RPSI07_RS10510 at 560.487 kb on + strand, within RPSI07_RS10510 at 560.868 kb on + strand at 560.868 kb on + strand at 560.868 kb on + strand at 561.134 kb on + strand, within RPSI07_RS10515 at 561.134 kb on + strand, within RPSI07_RS10515 at 561.134 kb on + strand, within RPSI07_RS10515 at 561.134 kb on + strand, within RPSI07_RS10515 at 561.134 kb on + strand, within RPSI07_RS10515 at 561.134 kb on + strand, within RPSI07_RS10515 at 561.170 kb on + strand, within RPSI07_RS10515 at 561.354 kb on - strand, within RPSI07_RS10515 at 561.374 kb on + strand, within RPSI07_RS10515 at 561.477 kb on - strand, within RPSI07_RS10515 at 561.599 kb on + strand, within RPSI07_RS10515 at 561.599 kb on + strand, within RPSI07_RS10515 at 561.599 kb on + strand, within RPSI07_RS10515 at 561.815 kb on + strand at 561.829 kb on + strand at 562.570 kb on + strand at 562.570 kb on + strand at 562.571 kb on - strand at 562.599 kb on + strand, within RPSI07_RS10525 at 562.621 kb on + strand, within RPSI07_RS10525 at 562.621 kb on + strand, within RPSI07_RS10525 at 562.647 kb on + strand, within RPSI07_RS10525
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Bacitracin remove 560,034 + RPSI07_RS10510 0.34 -0.3 560,313 + RPSI07_RS10510 0.54 -0.4 560,317 - RPSI07_RS10510 0.54 -0.7 560,456 + RPSI07_RS10510 0.64 -0.4 560,487 + RPSI07_RS10510 0.66 -1.0 560,868 + -0.6 560,868 + -0.4 560,868 + -0.1 561,134 + RPSI07_RS10515 0.20 +0.1 561,134 + RPSI07_RS10515 0.20 -0.1 561,134 + RPSI07_RS10515 0.20 +0.0 561,134 + RPSI07_RS10515 0.20 -0.1 561,134 + RPSI07_RS10515 0.20 +0.1 561,134 + RPSI07_RS10515 0.20 -0.6 561,170 + RPSI07_RS10515 0.25 -0.2 561,354 - RPSI07_RS10515 0.50 -0.1 561,374 + RPSI07_RS10515 0.52 +0.2 561,477 - RPSI07_RS10515 0.66 -0.1 561,599 + RPSI07_RS10515 0.82 +0.1 561,599 + RPSI07_RS10515 0.82 -0.1 561,599 + RPSI07_RS10515 0.82 +0.6 561,815 + -1.4 561,829 + -0.0 562,570 + -0.4 562,570 + +0.9 562,571 - -0.1 562,599 + RPSI07_RS10525 0.11 +1.1 562,621 + RPSI07_RS10525 0.15 +1.9 562,621 + RPSI07_RS10525 0.15 -1.7 562,647 + RPSI07_RS10525 0.20 +1.9
Or see this region's nucleotide sequence