Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS22425 and RPSI07_RS22430 are separated by 139 nucleotides RPSI07_RS22430 and RPSI07_RS22435 are separated by 210 nucleotides RPSI07_RS22435 and RPSI07_RS22440 are separated by 40 nucleotides RPSI07_RS22440 and RPSI07_RS22445 overlap by 32 nucleotides RPSI07_RS22445 and RPSI07_RS22450 are separated by 108 nucleotides
RPSI07_RS22425: RPSI07_RS22425 - bacterioferritin, at 3,188,589 to 3,189,068
_RS22425
RPSI07_RS22430: RPSI07_RS22430 - (2Fe-2S)-binding protein, at 3,189,208 to 3,189,483
_RS22430
RPSI07_RS22435: RPSI07_RS22435 - phosphoenolpyruvate--protein phosphotransferase, at 3,189,694 to 3,191,454
_RS22435
RPSI07_RS22440: RPSI07_RS22440 - HPr family phosphocarrier protein, at 3,191,495 to 3,191,764
_RS22440
RPSI07_RS22445: RPSI07_RS22445 - PTS fructose transporter subunit IIA, at 3,191,733 to 3,192,188
_RS22445
RPSI07_RS22450: RPSI07_RS22450 - glutathione synthase, at 3,192,297 to 3,193,271
_RS22450
Position (kb)
3189
3190
3191
3192 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3188.728 kb on + strand, within RPSI07_RS22425 at 3188.728 kb on + strand, within RPSI07_RS22425 at 3188.728 kb on + strand, within RPSI07_RS22425 at 3188.750 kb on - strand, within RPSI07_RS22425 at 3188.750 kb on - strand, within RPSI07_RS22425 at 3188.935 kb on + strand, within RPSI07_RS22425 at 3188.935 kb on + strand, within RPSI07_RS22425 at 3188.935 kb on + strand, within RPSI07_RS22425 at 3188.935 kb on + strand, within RPSI07_RS22425 at 3188.936 kb on - strand, within RPSI07_RS22425 at 3188.936 kb on - strand, within RPSI07_RS22425 at 3188.974 kb on + strand, within RPSI07_RS22425 at 3188.974 kb on + strand, within RPSI07_RS22425 at 3188.974 kb on + strand, within RPSI07_RS22425 at 3188.975 kb on - strand, within RPSI07_RS22425 at 3188.975 kb on - strand, within RPSI07_RS22425 at 3188.975 kb on - strand, within RPSI07_RS22425 at 3188.975 kb on - strand, within RPSI07_RS22425 at 3188.975 kb on - strand, within RPSI07_RS22425 at 3188.995 kb on + strand, within RPSI07_RS22425 at 3188.995 kb on + strand, within RPSI07_RS22425 at 3188.996 kb on - strand, within RPSI07_RS22425 at 3188.996 kb on - strand, within RPSI07_RS22425 at 3189.040 kb on + strand at 3189.056 kb on + strand at 3189.057 kb on - strand at 3189.274 kb on + strand, within RPSI07_RS22430 at 3189.274 kb on + strand, within RPSI07_RS22430 at 3189.275 kb on - strand, within RPSI07_RS22430 at 3189.479 kb on + strand at 3189.479 kb on + strand at 3189.479 kb on + strand at 3189.479 kb on + strand at 3189.480 kb on - strand at 3189.480 kb on - strand at 3189.480 kb on - strand at 3189.480 kb on - strand at 3189.528 kb on - strand at 3189.990 kb on - strand, within RPSI07_RS22435 at 3190.034 kb on + strand, within RPSI07_RS22435 at 3190.152 kb on - strand, within RPSI07_RS22435 at 3190.152 kb on - strand, within RPSI07_RS22435 at 3190.152 kb on - strand, within RPSI07_RS22435 at 3190.712 kb on + strand, within RPSI07_RS22435 at 3191.081 kb on + strand, within RPSI07_RS22435 at 3191.153 kb on + strand, within RPSI07_RS22435 at 3191.361 kb on - strand at 3191.361 kb on - strand at 3191.361 kb on - strand at 3191.394 kb on + strand at 3191.395 kb on - strand at 3191.395 kb on - strand at 3191.445 kb on + strand at 3191.446 kb on - strand at 3191.446 kb on - strand at 3191.465 kb on + strand at 3191.466 kb on - strand at 3191.496 kb on + strand at 3191.734 kb on + strand at 3191.734 kb on + strand at 3191.734 kb on + strand at 3191.735 kb on - strand at 3191.749 kb on + strand at 3191.750 kb on - strand at 3191.750 kb on - strand at 3191.750 kb on - strand at 3191.764 kb on + strand at 3191.764 kb on + strand at 3191.764 kb on + strand at 3191.764 kb on + strand at 3191.764 kb on + strand at 3191.765 kb on - strand at 3191.765 kb on - strand at 3191.765 kb on - strand at 3191.765 kb on - strand at 3191.765 kb on - strand at 3191.820 kb on + strand, within RPSI07_RS22445 at 3192.118 kb on + strand, within RPSI07_RS22445 at 3192.118 kb on + strand, within RPSI07_RS22445 at 3192.118 kb on + strand, within RPSI07_RS22445 at 3192.221 kb on + strand at 3192.221 kb on + strand at 3192.221 kb on + strand at 3192.221 kb on + strand at 3192.222 kb on - strand at 3192.222 kb on - strand at 3192.222 kb on - strand at 3192.273 kb on + strand at 3192.299 kb on - strand at 3192.299 kb on - strand at 3192.442 kb on + strand, within RPSI07_RS22450 at 3192.442 kb on + strand, within RPSI07_RS22450
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 3,188,728 + RPSI07_RS22425 0.29 -0.5 3,188,728 + RPSI07_RS22425 0.29 -0.5 3,188,728 + RPSI07_RS22425 0.29 +0.7 3,188,750 - RPSI07_RS22425 0.34 -0.2 3,188,750 - RPSI07_RS22425 0.34 -0.5 3,188,935 + RPSI07_RS22425 0.72 -0.1 3,188,935 + RPSI07_RS22425 0.72 -0.4 3,188,935 + RPSI07_RS22425 0.72 -0.3 3,188,935 + RPSI07_RS22425 0.72 +0.9 3,188,936 - RPSI07_RS22425 0.72 -0.1 3,188,936 - RPSI07_RS22425 0.72 +0.9 3,188,974 + RPSI07_RS22425 0.80 +0.2 3,188,974 + RPSI07_RS22425 0.80 -0.2 3,188,974 + RPSI07_RS22425 0.80 -0.1 3,188,975 - RPSI07_RS22425 0.80 +0.9 3,188,975 - RPSI07_RS22425 0.80 -1.4 3,188,975 - RPSI07_RS22425 0.80 -0.7 3,188,975 - RPSI07_RS22425 0.80 -0.7 3,188,975 - RPSI07_RS22425 0.80 +0.0 3,188,995 + RPSI07_RS22425 0.85 -0.4 3,188,995 + RPSI07_RS22425 0.85 -0.6 3,188,996 - RPSI07_RS22425 0.85 +0.2 3,188,996 - RPSI07_RS22425 0.85 +0.9 3,189,040 + +0.8 3,189,056 + +0.4 3,189,057 - +0.9 3,189,274 + RPSI07_RS22430 0.24 -0.5 3,189,274 + RPSI07_RS22430 0.24 +0.5 3,189,275 - RPSI07_RS22430 0.24 +1.7 3,189,479 + +0.3 3,189,479 + -0.1 3,189,479 + +0.1 3,189,479 + +1.9 3,189,480 - -0.2 3,189,480 - -1.4 3,189,480 - -0.5 3,189,480 - -0.1 3,189,528 - -2.3 3,189,990 - RPSI07_RS22435 0.17 +1.3 3,190,034 + RPSI07_RS22435 0.19 +0.2 3,190,152 - RPSI07_RS22435 0.26 -0.5 3,190,152 - RPSI07_RS22435 0.26 -0.7 3,190,152 - RPSI07_RS22435 0.26 +0.8 3,190,712 + RPSI07_RS22435 0.58 +0.9 3,191,081 + RPSI07_RS22435 0.79 +1.1 3,191,153 + RPSI07_RS22435 0.83 +0.7 3,191,361 - +0.9 3,191,361 - +0.4 3,191,361 - -0.1 3,191,394 + +0.2 3,191,395 - +1.2 3,191,395 - +0.9 3,191,445 + -0.0 3,191,446 - +0.6 3,191,446 - +0.1 3,191,465 + +0.4 3,191,466 - +0.6 3,191,496 + -0.0 3,191,734 + +0.6 3,191,734 + +0.1 3,191,734 + +0.1 3,191,735 - +1.3 3,191,749 + -0.8 3,191,750 - +0.8 3,191,750 - +0.2 3,191,750 - +0.3 3,191,764 + +0.7 3,191,764 + +0.9 3,191,764 + -0.3 3,191,764 + +0.8 3,191,764 + -0.4 3,191,765 - -0.5 3,191,765 - -0.8 3,191,765 - -0.2 3,191,765 - -0.7 3,191,765 - -0.2 3,191,820 + RPSI07_RS22445 0.19 -0.7 3,192,118 + RPSI07_RS22445 0.84 +0.2 3,192,118 + RPSI07_RS22445 0.84 -0.1 3,192,118 + RPSI07_RS22445 0.84 -0.7 3,192,221 + -1.5 3,192,221 + +1.2 3,192,221 + +0.0 3,192,221 + +0.9 3,192,222 - -0.1 3,192,222 - -0.9 3,192,222 - -0.3 3,192,273 + +0.7 3,192,299 - -0.6 3,192,299 - -0.2 3,192,442 + RPSI07_RS22450 0.15 -0.5 3,192,442 + RPSI07_RS22450 0.15 -0.5
Or see this region's nucleotide sequence