Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS22415 and RPSI07_RS22420 are separated by 72 nucleotides RPSI07_RS22420 and RPSI07_RS22425 are separated by 67 nucleotides RPSI07_RS22425 and RPSI07_RS22430 are separated by 139 nucleotides
RPSI07_RS22415: RPSI07_RS22415 - S41 family peptidase, at 3,186,083 to 3,187,699
_RS22415
RPSI07_RS22420: RPSI07_RS22420 - molybdopterin-synthase adenylyltransferase MoeB, at 3,187,772 to 3,188,521
_RS22420
RPSI07_RS22425: RPSI07_RS22425 - bacterioferritin, at 3,188,589 to 3,189,068
_RS22425
RPSI07_RS22430: RPSI07_RS22430 - (2Fe-2S)-binding protein, at 3,189,208 to 3,189,483
_RS22430
Position (kb)
3187
3188
3189 Strain fitness (log2 ratio)
-1
0
1
2 at 3186.908 kb on + strand, within RPSI07_RS22415 at 3186.908 kb on + strand, within RPSI07_RS22415 at 3186.930 kb on - strand, within RPSI07_RS22415 at 3187.017 kb on - strand, within RPSI07_RS22415 at 3187.017 kb on - strand, within RPSI07_RS22415 at 3187.166 kb on + strand, within RPSI07_RS22415 at 3187.169 kb on + strand, within RPSI07_RS22415 at 3188.623 kb on + strand at 3188.624 kb on - strand at 3188.624 kb on - strand at 3188.728 kb on + strand, within RPSI07_RS22425 at 3188.728 kb on + strand, within RPSI07_RS22425 at 3188.728 kb on + strand, within RPSI07_RS22425 at 3188.750 kb on - strand, within RPSI07_RS22425 at 3188.750 kb on - strand, within RPSI07_RS22425 at 3188.935 kb on + strand, within RPSI07_RS22425 at 3188.935 kb on + strand, within RPSI07_RS22425 at 3188.935 kb on + strand, within RPSI07_RS22425 at 3188.935 kb on + strand, within RPSI07_RS22425 at 3188.936 kb on - strand, within RPSI07_RS22425 at 3188.936 kb on - strand, within RPSI07_RS22425 at 3188.974 kb on + strand, within RPSI07_RS22425 at 3188.974 kb on + strand, within RPSI07_RS22425 at 3188.974 kb on + strand, within RPSI07_RS22425 at 3188.975 kb on - strand, within RPSI07_RS22425 at 3188.975 kb on - strand, within RPSI07_RS22425 at 3188.975 kb on - strand, within RPSI07_RS22425 at 3188.975 kb on - strand, within RPSI07_RS22425 at 3188.975 kb on - strand, within RPSI07_RS22425 at 3188.995 kb on + strand, within RPSI07_RS22425 at 3188.995 kb on + strand, within RPSI07_RS22425 at 3188.996 kb on - strand, within RPSI07_RS22425 at 3188.996 kb on - strand, within RPSI07_RS22425 at 3189.040 kb on + strand at 3189.056 kb on + strand at 3189.057 kb on - strand at 3189.274 kb on + strand, within RPSI07_RS22430 at 3189.274 kb on + strand, within RPSI07_RS22430 at 3189.275 kb on - strand, within RPSI07_RS22430 at 3189.479 kb on + strand at 3189.479 kb on + strand at 3189.479 kb on + strand at 3189.479 kb on + strand at 3189.480 kb on - strand at 3189.480 kb on - strand at 3189.480 kb on - strand at 3189.480 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin remove 3,186,908 + RPSI07_RS22415 0.51 +0.9 3,186,908 + RPSI07_RS22415 0.51 -0.9 3,186,930 - RPSI07_RS22415 0.52 -0.7 3,187,017 - RPSI07_RS22415 0.58 -1.4 3,187,017 - RPSI07_RS22415 0.58 +0.9 3,187,166 + RPSI07_RS22415 0.67 -0.1 3,187,169 + RPSI07_RS22415 0.67 -1.4 3,188,623 + -1.0 3,188,624 - -0.1 3,188,624 - +0.9 3,188,728 + RPSI07_RS22425 0.29 -0.5 3,188,728 + RPSI07_RS22425 0.29 -0.5 3,188,728 + RPSI07_RS22425 0.29 +0.7 3,188,750 - RPSI07_RS22425 0.34 -0.2 3,188,750 - RPSI07_RS22425 0.34 -0.5 3,188,935 + RPSI07_RS22425 0.72 -0.1 3,188,935 + RPSI07_RS22425 0.72 -0.4 3,188,935 + RPSI07_RS22425 0.72 -0.3 3,188,935 + RPSI07_RS22425 0.72 +0.9 3,188,936 - RPSI07_RS22425 0.72 -0.1 3,188,936 - RPSI07_RS22425 0.72 +0.9 3,188,974 + RPSI07_RS22425 0.80 +0.2 3,188,974 + RPSI07_RS22425 0.80 -0.2 3,188,974 + RPSI07_RS22425 0.80 -0.1 3,188,975 - RPSI07_RS22425 0.80 +0.9 3,188,975 - RPSI07_RS22425 0.80 -1.4 3,188,975 - RPSI07_RS22425 0.80 -0.7 3,188,975 - RPSI07_RS22425 0.80 -0.7 3,188,975 - RPSI07_RS22425 0.80 +0.0 3,188,995 + RPSI07_RS22425 0.85 -0.4 3,188,995 + RPSI07_RS22425 0.85 -0.6 3,188,996 - RPSI07_RS22425 0.85 +0.2 3,188,996 - RPSI07_RS22425 0.85 +0.9 3,189,040 + +0.8 3,189,056 + +0.4 3,189,057 - +0.9 3,189,274 + RPSI07_RS22430 0.24 -0.5 3,189,274 + RPSI07_RS22430 0.24 +0.5 3,189,275 - RPSI07_RS22430 0.24 +1.7 3,189,479 + +0.3 3,189,479 + -0.1 3,189,479 + +0.1 3,189,479 + +1.9 3,189,480 - -0.2 3,189,480 - -1.4 3,189,480 - -0.5 3,189,480 - -0.1
Or see this region's nucleotide sequence