Experiment: SMSA with Ralstonia_antibiotic_mix
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS08035 and RPSI07_RS08040 overlap by 1 nucleotides RPSI07_RS08040 and RPSI07_RS08045 are separated by 244 nucleotides RPSI07_RS08045 and RPSI07_RS08050 are separated by 34 nucleotides RPSI07_RS08050 and RPSI07_RS08055 overlap by 25 nucleotides
RPSI07_RS08035: RPSI07_RS08035 - alpha/beta hydrolase, at 41,640 to 42,419
_RS08035
RPSI07_RS08040: RPSI07_RS08040 - hypothetical protein, at 42,419 to 43,033
_RS08040
RPSI07_RS08045: RPSI07_RS08045 - membrane protein, at 43,278 to 44,063
_RS08045
RPSI07_RS08050: RPSI07_RS08050 - DNA-binding response regulator, at 44,098 to 44,763
_RS08050
RPSI07_RS08055: RPSI07_RS08055 - two-component sensor histidine kinase, at 44,739 to 46,079
_RS08055
Position (kb) 43
44
45 Strain fitness (log2 ratio) -1
0
1 at 42.386 kb on + strand at 42.386 kb on + strand at 42.386 kb on + strand at 42.387 kb on - strand at 42.387 kb on - strand at 42.474 kb on + strand at 42.474 kb on + strand at 43.100 kb on + strand at 43.100 kb on + strand at 43.100 kb on + strand at 43.100 kb on + strand at 43.100 kb on + strand at 43.100 kb on + strand at 43.100 kb on + strand at 43.101 kb on - strand at 43.101 kb on - strand at 43.101 kb on - strand at 43.101 kb on - strand at 43.101 kb on - strand at 43.101 kb on - strand at 43.101 kb on - strand at 43.101 kb on - strand at 43.221 kb on + strand at 43.222 kb on - strand at 43.222 kb on - strand at 43.500 kb on + strand, within RPSI07_RS08045 at 43.500 kb on + strand, within RPSI07_RS08045 at 43.500 kb on + strand, within RPSI07_RS08045 at 43.500 kb on + strand, within RPSI07_RS08045 at 43.515 kb on + strand, within RPSI07_RS08045 at 43.515 kb on + strand, within RPSI07_RS08045 at 43.516 kb on - strand, within RPSI07_RS08045 at 43.644 kb on + strand, within RPSI07_RS08045 at 43.710 kb on + strand, within RPSI07_RS08045 at 43.842 kb on + strand, within RPSI07_RS08045 at 43.843 kb on - strand, within RPSI07_RS08045 at 43.845 kb on + strand, within RPSI07_RS08045 at 43.845 kb on + strand, within RPSI07_RS08045 at 43.845 kb on + strand, within RPSI07_RS08045 at 43.845 kb on + strand, within RPSI07_RS08045 at 43.846 kb on - strand, within RPSI07_RS08045 at 43.846 kb on - strand, within RPSI07_RS08045 at 43.846 kb on - strand, within RPSI07_RS08045 at 43.846 kb on - strand, within RPSI07_RS08045 at 43.846 kb on - strand, within RPSI07_RS08045 at 44.019 kb on + strand at 44.019 kb on + strand at 44.020 kb on - strand at 44.021 kb on + strand at 44.021 kb on + strand at 44.021 kb on + strand at 44.021 kb on + strand at 44.170 kb on + strand, within RPSI07_RS08050 at 44.170 kb on + strand, within RPSI07_RS08050 at 44.230 kb on + strand, within RPSI07_RS08050 at 44.230 kb on + strand, within RPSI07_RS08050 at 44.283 kb on - strand, within RPSI07_RS08050 at 44.283 kb on - strand, within RPSI07_RS08050 at 44.283 kb on - strand, within RPSI07_RS08050 at 44.389 kb on + strand, within RPSI07_RS08050 at 44.389 kb on + strand, within RPSI07_RS08050 at 44.389 kb on + strand, within RPSI07_RS08050 at 44.389 kb on + strand, within RPSI07_RS08050 at 44.389 kb on + strand, within RPSI07_RS08050 at 44.390 kb on - strand, within RPSI07_RS08050 at 44.635 kb on + strand, within RPSI07_RS08050 at 44.635 kb on + strand, within RPSI07_RS08050 at 44.635 kb on + strand, within RPSI07_RS08050 at 44.635 kb on + strand, within RPSI07_RS08050 at 44.635 kb on + strand, within RPSI07_RS08050 at 44.635 kb on + strand, within RPSI07_RS08050 at 44.636 kb on - strand, within RPSI07_RS08050 at 44.636 kb on - strand, within RPSI07_RS08050
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix remove 42,386 + -0.3 42,386 + +0.3 42,386 + +1.0 42,387 - -0.1 42,387 - +1.0 42,474 + -0.2 42,474 + -0.9 43,100 + -0.9 43,100 + -1.2 43,100 + -0.3 43,100 + +0.1 43,100 + -1.0 43,100 + +1.0 43,100 + -1.3 43,101 - -0.7 43,101 - +1.0 43,101 - -0.6 43,101 - +0.7 43,101 - +0.2 43,101 - +0.2 43,101 - -1.7 43,101 - +0.3 43,221 + +1.0 43,222 - +0.3 43,222 - -0.5 43,500 + RPSI07_RS08045 0.28 -0.1 43,500 + RPSI07_RS08045 0.28 -0.6 43,500 + RPSI07_RS08045 0.28 +0.5 43,500 + RPSI07_RS08045 0.28 -0.6 43,515 + RPSI07_RS08045 0.30 +0.4 43,515 + RPSI07_RS08045 0.30 -0.6 43,516 - RPSI07_RS08045 0.30 -0.1 43,644 + RPSI07_RS08045 0.47 +0.7 43,710 + RPSI07_RS08045 0.55 -1.6 43,842 + RPSI07_RS08045 0.72 +0.3 43,843 - RPSI07_RS08045 0.72 -0.7 43,845 + RPSI07_RS08045 0.72 +0.6 43,845 + RPSI07_RS08045 0.72 -0.2 43,845 + RPSI07_RS08045 0.72 +0.1 43,845 + RPSI07_RS08045 0.72 -0.4 43,846 - RPSI07_RS08045 0.72 +1.0 43,846 - RPSI07_RS08045 0.72 -0.3 43,846 - RPSI07_RS08045 0.72 -0.5 43,846 - RPSI07_RS08045 0.72 +0.4 43,846 - RPSI07_RS08045 0.72 -1.0 44,019 + -0.3 44,019 + -0.9 44,020 - +0.5 44,021 + +0.3 44,021 + -0.2 44,021 + +0.7 44,021 + +0.5 44,170 + RPSI07_RS08050 0.11 +0.0 44,170 + RPSI07_RS08050 0.11 +0.7 44,230 + RPSI07_RS08050 0.20 -1.0 44,230 + RPSI07_RS08050 0.20 -0.6 44,283 - RPSI07_RS08050 0.28 -0.2 44,283 - RPSI07_RS08050 0.28 -0.3 44,283 - RPSI07_RS08050 0.28 +0.7 44,389 + RPSI07_RS08050 0.44 +0.0 44,389 + RPSI07_RS08050 0.44 -0.6 44,389 + RPSI07_RS08050 0.44 +0.8 44,389 + RPSI07_RS08050 0.44 -0.6 44,389 + RPSI07_RS08050 0.44 +0.3 44,390 - RPSI07_RS08050 0.44 -0.2 44,635 + RPSI07_RS08050 0.81 -0.3 44,635 + RPSI07_RS08050 0.81 -0.8 44,635 + RPSI07_RS08050 0.81 +1.4 44,635 + RPSI07_RS08050 0.81 +1.0 44,635 + RPSI07_RS08050 0.81 +1.4 44,635 + RPSI07_RS08050 0.81 +0.7 44,636 - RPSI07_RS08050 0.81 -0.1 44,636 - RPSI07_RS08050 0.81 +0.0
Or see this region's nucleotide sequence