Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS17680 and RPSI07_RS17685 overlap by 4 nucleotides RPSI07_RS17685 and RPSI07_RS17690 are separated by 207 nucleotides RPSI07_RS17690 and RPSI07_RS17695 are separated by 89 nucleotides
RPSI07_RS17680: RPSI07_RS17680 - transcription termination/antitermination protein NusA, at 2,170,736 to 2,172,211
_RS17680
RPSI07_RS17685: RPSI07_RS17685 - ribosome maturation factor RimP, at 2,172,208 to 2,172,696
_RS17685
RPSI07_RS17690: RPSI07_RS17690 - 23S rRNA pseudouridylate synthase B, at 2,172,904 to 2,174,688
_RS17690
RPSI07_RS17695: RPSI07_RS17695 - SMC-Scp complex subunit ScpB, at 2,174,778 to 2,175,671
_RS17695
Position (kb)
2172
2173
2174
2175 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 2173.091 kb on + strand, within RPSI07_RS17690 at 2173.512 kb on - strand, within RPSI07_RS17690 at 2173.847 kb on + strand, within RPSI07_RS17690 at 2174.120 kb on + strand, within RPSI07_RS17690 at 2174.121 kb on - strand, within RPSI07_RS17690 at 2174.139 kb on - strand, within RPSI07_RS17690 at 2174.467 kb on - strand, within RPSI07_RS17690 at 2174.688 kb on + strand at 2174.688 kb on + strand at 2174.688 kb on + strand at 2174.689 kb on - strand at 2174.689 kb on - strand at 2174.689 kb on - strand at 2174.689 kb on - strand at 2174.695 kb on - strand at 2174.695 kb on - strand at 2174.695 kb on - strand at 2174.779 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 2,173,091 + RPSI07_RS17690 0.10 +0.6 2,173,512 - RPSI07_RS17690 0.34 +0.5 2,173,847 + RPSI07_RS17690 0.53 +0.9 2,174,120 + RPSI07_RS17690 0.68 +1.0 2,174,121 - RPSI07_RS17690 0.68 +3.7 2,174,139 - RPSI07_RS17690 0.69 +4.8 2,174,467 - RPSI07_RS17690 0.88 +3.6 2,174,688 + +1.0 2,174,688 + +3.6 2,174,688 + +2.8 2,174,689 - +4.8 2,174,689 - +2.9 2,174,689 - +3.2 2,174,689 - +4.0 2,174,695 - +3.5 2,174,695 - +3.0 2,174,695 - +3.9 2,174,779 + +3.9
Or see this region's nucleotide sequence