Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS16660 and RPSI07_RS16665 are separated by 235 nucleotides RPSI07_RS16665 and RPSI07_RS16670 overlap by 4 nucleotides
RPSI07_RS16660: RPSI07_RS16660 - hybrid non-ribosomal peptide synthetase/type I polyketide synthase, at 1,929,654 to 1,942,466
_RS16660
RPSI07_RS16665: RPSI07_RS16665 - thiazolinyl imide reductase, at 1,942,702 to 1,943,865
_RS16665
RPSI07_RS16670: RPSI07_RS16670 - peptide biosynthesis thioesterase, at 1,943,862 to 1,945,589
_RS16670
Position (kb)
1942
1943
1944 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1941.773 kb on - strand at 1941.840 kb on + strand at 1941.910 kb on - strand at 1941.910 kb on - strand at 1942.464 kb on + strand at 1942.464 kb on + strand at 1942.465 kb on - strand at 1942.465 kb on - strand at 1942.465 kb on - strand at 1942.732 kb on + strand at 1942.732 kb on + strand at 1942.732 kb on + strand at 1942.732 kb on + strand at 1942.733 kb on - strand at 1942.733 kb on - strand at 1942.733 kb on - strand at 1942.733 kb on - strand at 1942.733 kb on - strand at 1942.733 kb on - strand at 1942.733 kb on - strand at 1942.733 kb on - strand at 1942.771 kb on + strand at 1942.771 kb on + strand at 1942.772 kb on - strand at 1942.772 kb on - strand at 1942.772 kb on - strand at 1943.030 kb on + strand, within RPSI07_RS16665 at 1943.030 kb on + strand, within RPSI07_RS16665 at 1943.030 kb on + strand, within RPSI07_RS16665 at 1943.030 kb on + strand, within RPSI07_RS16665 at 1943.030 kb on + strand, within RPSI07_RS16665 at 1943.031 kb on - strand, within RPSI07_RS16665 at 1943.031 kb on - strand, within RPSI07_RS16665 at 1943.031 kb on - strand, within RPSI07_RS16665 at 1943.031 kb on - strand, within RPSI07_RS16665 at 1943.031 kb on - strand, within RPSI07_RS16665 at 1943.122 kb on + strand, within RPSI07_RS16665 at 1943.158 kb on + strand, within RPSI07_RS16665 at 1943.158 kb on + strand, within RPSI07_RS16665 at 1943.227 kb on + strand, within RPSI07_RS16665 at 1943.369 kb on + strand, within RPSI07_RS16665 at 1943.369 kb on + strand, within RPSI07_RS16665 at 1943.370 kb on - strand, within RPSI07_RS16665 at 1943.370 kb on - strand, within RPSI07_RS16665 at 1943.396 kb on - strand, within RPSI07_RS16665 at 1943.488 kb on + strand, within RPSI07_RS16665 at 1943.518 kb on + strand, within RPSI07_RS16665 at 1943.518 kb on + strand, within RPSI07_RS16665 at 1943.558 kb on - strand, within RPSI07_RS16665 at 1943.558 kb on - strand, within RPSI07_RS16665 at 1943.846 kb on - strand at 1943.846 kb on - strand at 1943.882 kb on + strand at 1943.883 kb on - strand at 1943.887 kb on + strand at 1943.887 kb on + strand at 1943.887 kb on + strand at 1943.887 kb on + strand at 1943.888 kb on - strand at 1943.888 kb on - strand at 1943.888 kb on - strand at 1943.953 kb on - strand at 1944.007 kb on - strand at 1944.007 kb on - strand at 1944.007 kb on - strand at 1944.633 kb on + strand, within RPSI07_RS16670 at 1944.634 kb on - strand, within RPSI07_RS16670 at 1944.634 kb on - strand, within RPSI07_RS16670
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 1,941,773 - -1.4 1,941,840 + +0.0 1,941,910 - -1.4 1,941,910 - -0.8 1,942,464 + +0.2 1,942,464 + -1.0 1,942,465 - -0.4 1,942,465 - +0.5 1,942,465 - +0.8 1,942,732 + +1.6 1,942,732 + +0.4 1,942,732 + -1.2 1,942,732 + +2.5 1,942,733 - +1.5 1,942,733 - -0.2 1,942,733 - +1.2 1,942,733 - -1.0 1,942,733 - -0.0 1,942,733 - -0.4 1,942,733 - -0.1 1,942,733 - +0.0 1,942,771 + +0.1 1,942,771 + -0.6 1,942,772 - -1.4 1,942,772 - -1.3 1,942,772 - +1.0 1,943,030 + RPSI07_RS16665 0.28 -1.2 1,943,030 + RPSI07_RS16665 0.28 -1.0 1,943,030 + RPSI07_RS16665 0.28 -0.9 1,943,030 + RPSI07_RS16665 0.28 -2.6 1,943,030 + RPSI07_RS16665 0.28 +1.4 1,943,031 - RPSI07_RS16665 0.28 +3.8 1,943,031 - RPSI07_RS16665 0.28 +1.4 1,943,031 - RPSI07_RS16665 0.28 -0.0 1,943,031 - RPSI07_RS16665 0.28 +4.7 1,943,031 - RPSI07_RS16665 0.28 -0.6 1,943,122 + RPSI07_RS16665 0.36 +2.0 1,943,158 + RPSI07_RS16665 0.39 -1.2 1,943,158 + RPSI07_RS16665 0.39 +1.7 1,943,227 + RPSI07_RS16665 0.45 +0.2 1,943,369 + RPSI07_RS16665 0.57 -0.4 1,943,369 + RPSI07_RS16665 0.57 +0.9 1,943,370 - RPSI07_RS16665 0.57 +0.4 1,943,370 - RPSI07_RS16665 0.57 +1.2 1,943,396 - RPSI07_RS16665 0.60 -0.3 1,943,488 + RPSI07_RS16665 0.68 +0.1 1,943,518 + RPSI07_RS16665 0.70 -2.0 1,943,518 + RPSI07_RS16665 0.70 +0.9 1,943,558 - RPSI07_RS16665 0.74 +2.5 1,943,558 - RPSI07_RS16665 0.74 -1.0 1,943,846 - -1.6 1,943,846 - +1.2 1,943,882 + -1.8 1,943,883 - +0.6 1,943,887 + -1.3 1,943,887 + +0.6 1,943,887 + +0.4 1,943,887 + -0.7 1,943,888 - -2.1 1,943,888 - +2.0 1,943,888 - -0.3 1,943,953 - -0.4 1,944,007 - +0.1 1,944,007 - +0.5 1,944,007 - -0.0 1,944,633 + RPSI07_RS16670 0.45 +1.5 1,944,634 - RPSI07_RS16670 0.45 +0.9 1,944,634 - RPSI07_RS16670 0.45 -1.6
Or see this region's nucleotide sequence