Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS10585 and RPSI07_RS10590 overlap by 4 nucleotides RPSI07_RS10590 and RPSI07_RS10595 are separated by 25 nucleotides RPSI07_RS10595 and RPSI07_RS10600 are separated by 170 nucleotides
RPSI07_RS10585: RPSI07_RS10585 - energy-dependent translational throttle protein EttA, at 577,967 to 579,634
_RS10585
RPSI07_RS10590: RPSI07_RS10590 - CDGSH iron-sulfur domain-containing protein, at 579,631 to 579,861
_RS10590
RPSI07_RS10595: RPSI07_RS10595 - aminoacyl-tRNA deacylase, at 579,887 to 580,375
_RS10595
RPSI07_RS10600: RPSI07_RS10600 - hypothetical protein, at 580,546 to 580,860
_RS10600
Position (kb) 579
580 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 578.696 kb on + strand, within RPSI07_RS10585 at 578.696 kb on + strand, within RPSI07_RS10585 at 578.696 kb on + strand, within RPSI07_RS10585 at 578.696 kb on + strand, within RPSI07_RS10585 at 579.134 kb on + strand, within RPSI07_RS10585 at 579.245 kb on + strand, within RPSI07_RS10585 at 579.246 kb on - strand, within RPSI07_RS10585 at 579.248 kb on - strand, within RPSI07_RS10585 at 579.767 kb on + strand, within RPSI07_RS10590 at 580.006 kb on - strand, within RPSI07_RS10595 at 580.006 kb on - strand, within RPSI07_RS10595 at 580.041 kb on + strand, within RPSI07_RS10595 at 580.042 kb on - strand, within RPSI07_RS10595 at 580.042 kb on - strand, within RPSI07_RS10595 at 580.045 kb on + strand, within RPSI07_RS10595 at 580.045 kb on + strand, within RPSI07_RS10595 at 580.045 kb on + strand, within RPSI07_RS10595 at 580.057 kb on - strand, within RPSI07_RS10595 at 580.057 kb on - strand, within RPSI07_RS10595 at 580.143 kb on + strand, within RPSI07_RS10595 at 580.212 kb on + strand, within RPSI07_RS10595 at 580.213 kb on - strand, within RPSI07_RS10595 at 580.218 kb on + strand, within RPSI07_RS10595 at 580.218 kb on + strand, within RPSI07_RS10595 at 580.218 kb on + strand, within RPSI07_RS10595 at 580.218 kb on + strand, within RPSI07_RS10595 at 580.218 kb on + strand, within RPSI07_RS10595 at 580.218 kb on + strand, within RPSI07_RS10595 at 580.218 kb on + strand, within RPSI07_RS10595 at 580.219 kb on - strand, within RPSI07_RS10595 at 580.219 kb on - strand, within RPSI07_RS10595 at 580.219 kb on - strand, within RPSI07_RS10595 at 580.219 kb on - strand, within RPSI07_RS10595 at 580.219 kb on - strand, within RPSI07_RS10595 at 580.219 kb on - strand, within RPSI07_RS10595 at 580.323 kb on + strand, within RPSI07_RS10595 at 580.435 kb on + strand at 580.706 kb on + strand, within RPSI07_RS10600 at 580.706 kb on + strand, within RPSI07_RS10600 at 580.707 kb on - strand, within RPSI07_RS10600 at 580.749 kb on - strand, within RPSI07_RS10600 at 580.749 kb on - strand, within RPSI07_RS10600
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 578,696 + RPSI07_RS10585 0.44 -0.2 578,696 + RPSI07_RS10585 0.44 -0.7 578,696 + RPSI07_RS10585 0.44 +2.2 578,696 + RPSI07_RS10585 0.44 -1.1 579,134 + RPSI07_RS10585 0.70 -0.2 579,245 + RPSI07_RS10585 0.77 -0.6 579,246 - RPSI07_RS10585 0.77 -0.4 579,248 - RPSI07_RS10585 0.77 +0.6 579,767 + RPSI07_RS10590 0.59 -0.3 580,006 - RPSI07_RS10595 0.24 +1.2 580,006 - RPSI07_RS10595 0.24 -0.1 580,041 + RPSI07_RS10595 0.31 +1.2 580,042 - RPSI07_RS10595 0.32 +1.7 580,042 - RPSI07_RS10595 0.32 -0.8 580,045 + RPSI07_RS10595 0.32 -0.5 580,045 + RPSI07_RS10595 0.32 +1.3 580,045 + RPSI07_RS10595 0.32 +1.0 580,057 - RPSI07_RS10595 0.35 +1.2 580,057 - RPSI07_RS10595 0.35 +2.4 580,143 + RPSI07_RS10595 0.52 -1.7 580,212 + RPSI07_RS10595 0.66 -2.8 580,213 - RPSI07_RS10595 0.67 +2.2 580,218 + RPSI07_RS10595 0.68 -0.8 580,218 + RPSI07_RS10595 0.68 -1.7 580,218 + RPSI07_RS10595 0.68 -0.7 580,218 + RPSI07_RS10595 0.68 +0.2 580,218 + RPSI07_RS10595 0.68 -1.7 580,218 + RPSI07_RS10595 0.68 +1.0 580,218 + RPSI07_RS10595 0.68 +0.0 580,219 - RPSI07_RS10595 0.68 +0.6 580,219 - RPSI07_RS10595 0.68 -1.4 580,219 - RPSI07_RS10595 0.68 -0.0 580,219 - RPSI07_RS10595 0.68 +0.2 580,219 - RPSI07_RS10595 0.68 -0.4 580,219 - RPSI07_RS10595 0.68 +1.2 580,323 + RPSI07_RS10595 0.89 +1.2 580,435 + -1.1 580,706 + RPSI07_RS10600 0.51 +0.2 580,706 + RPSI07_RS10600 0.51 +1.2 580,707 - RPSI07_RS10600 0.51 +1.2 580,749 - RPSI07_RS10600 0.64 -0.2 580,749 - RPSI07_RS10600 0.64 +1.6
Or see this region's nucleotide sequence